Diagnosing Human Anisakiasis: Recombinant Ani s 1 and Ani s 7 Allergens versus the UniCAP 100 Fluorescence Enzyme Immunoassay

Anadón, A. M.; Rodríguez, Esperanza; Gárate, María T.; Cuéllar, C.; Romarís, F.; Chivato, T.; Rodero, M.; González-Dı́az, Humberto; Ubeira, Florencio M.

doi:10.1128/cvi.00443-09

Cited by 35 publications

(64 citation statements)

References 31 publications

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“…Ani s 7 has repeatedly been recognized as the most important Anisakis allergen as it is the only one recognized by more than 90% of patients [12,17,18]. Moreover, unlike for Ani s 1, the percentage of patients with IgE antibodies to Ani s 7 was consistently high in patients with acute Anisakis infections (gastroallergic anisakiosis) or with Anisakis sensitization associated with chronic urticaria [19].…”

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confidence: 99%

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Travelling with Anisakis Allergens

Ubeira

2014

Int Arch Allergy Immunol

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confidence: 99%

“…ImmunoCap and Western blot) [10], defined antigens recognized by monoclonal antibodies [11], and, more recently, recombinant allergens [12]. To date, 12 Anisakis allergens (Ani s 1 to Ani s 12) have been officially recognized by the WHO/IUIS Allergen Nomenclature Subcommittee (http://www.allergen.org/).…”

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confidence: 99%

Travelling with Anisakis Allergens

Ubeira

2014

Int Arch Allergy Immunol

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“…Excretory/secretory antigens are secreted by the parasite to help penetrate the gut mucosa and are probably the antigens involved in stimulation of the host immune system during active infection (Valls et al 2005). In an experimental study, Anadón et al demonstrated that anti-Ani s 7 IgE antibodies induced by live Anisakis larvae peaked on day 30 postinfection, decreasing over the course of two months (Anadon et al 2009), and that their presence could constitute a possible recent infection (Anadon et al 2010). In the present study, 7.46% (5/67) of volunteers were seroreactive to excretory/secretory antigens.…”

Section: Discussionmentioning

confidence: 50%

“…Although several Anisakis simplex allergens (Park et al 2012) have been identified, many unknown allergens may still exist (Kobayashi et al 2011). As there is no serological diagnostic technique for all forms of anisakiasis, excretory/secretory antibodies are currently recognized as the most specific and sensitive method for the diagnosis of human infection (Anadon et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niterói, Brazil

Figueiredo

Vericimo

Cardoso

et al. 2013

Acta Parasitologica

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Although the incidence of anisakiasis is rising worldwide, its frequency is still unknown in Brazil. The aim of this study was to verify immunoreactivity to Anisakis simplex antigens in healthy adults and determine its possible relationship with frequency of fish consumption and allergy symptoms. A prospective cross-sectional study was carried out with 67 volunteers recruited from a military facility in Niterói, Brazil. The subjects completed a structured questionnaire and serum titers of specific anti-Anisakis IgE and IgG antibodies were measured. The association between frequency of fish intake and IgE reactivity was evaluated by Fisher's exact test. Almost all subjects (97.0%, 65/67) that consumed seafood; 64.6% (42/65) ate fish at least once weekly. Of all seafood consumers, 56.9% (37/65) reported allergy symptoms, being gut allergies most often cited (35.5%). IgE seroreactivity to Anisakis simplex was found in 20.9% of subjects (14/67), with 13.4% (9/67) reacting exclusively to somatic antigen, 3.0% (2/67) exclusively to excretory/secretory antigens and 4.5% (3/67) to both antigens. There was a significant association between frequency of fish consumption and positive serology (p = 0.019). An immunoblot assay for Anisakis antigens showed different positive bands for IgG. The direct relationship between ELISA reactivity and frequency of fish intake and absence of association with allergy symptoms suggests previous contact with Anisakis simplex antigens.

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“…Serological studies have been used to detect Anisakis-induced IgE. 4,6,16,17 This antibody, however, lacks specificity as a result of cross-reactivity with other nematode antigens. The serological test is not generally available and it is of limited benefit in early diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Anisakiasis Causing Acute Dysentery in Malaysia

Amir

Ngui

Ismail

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Human anisakiasis is a zoonosis acquired by eating raw or undercooked infected seafood. Herein, we report a case of acute dysentery caused by anisakiasis in a 64-year-old man in Malaysia. A colonoscopy was performed and a nematode larva was found penetrating the mucosa of the ascending colon. Bleeding was observed at the site of penetration. Y-shaped lateral epidermal cords were seen from the cross section of the worm, which is a prominent feature of Anisakis larva. Molecular analysis using polymerase chain reaction of cytochrome oxidase 2 (cox2) gene confirmed the specimen to be larva of Anisakis simplex.

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Diagnosing Human Anisakiasis: Recombinant Ani s 1 and Ani s 7 Allergens versus the UniCAP 100 Fluorescence Enzyme Immunoassay

Cited by 35 publications

References 31 publications

Travelling with <b><i>Anisakis</i></b> Allergens

Travelling with <b><i>Anisakis</i></b> Allergens

Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niterói, Brazil

Anisakiasis Causing Acute Dysentery in Malaysia

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