Novel Senecavirus A in Swine with Vesicular Disease, United States, July 2015

Guo, Baoqing; Piñeyro, Pablo; Rademacher, Christopher; Zheng, Ying; Li, Ganwu; Yuan, Jian qin; Hoang, Hai; Gauger, Phillip C.; Madson, Darin M.; Schwartz, Kent; Canning, Paisley; Arruda, Bailey; Cooper, Vickie L.; Baum, David H.; Linhares, Daniel; Main, Rodger; Yoon, Kyoung‐Jin

doi:10.3201/eid2207.151758

Cited by 72 publications

(47 citation statements)

References 6 publications

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“…Foreign animal disease investigations indicated that other vesicular viral pathogens, such as foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), vesicular stomatitis virus (VSV), and vesicular exanthema of swine virus (VESV), were negative in these cases [10]. Subsequently, SVV detection was reported by other laboratories in the U.S. [11][12][13][14][15][16], China [17][18][19][20][21], Canada [22], Thailand [23], and Colombia [24]. Vesicular lesions were induced in pigs following experimental inoculation with the contemporary U.S. isolates of SVV [25,26], confirming that SVV is a vesicular viral pathogen.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

et al. 2019

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Background Seneca Valley virus (SVV) has emerged in multiple countries in recent years. SVV infection can cause vesicular lesions clinically indistinguishable from those caused by other vesicular disease viruses, such as foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), vesicular stomatitis virus (VSV), and vesicular exanthema of swine virus (VESV). Sensitive and specific RT-PCR assays for the SVV detection is necessary for differential diagnosis. Real-time RT-PCR (rRT-PCR) has been used for the detection of many RNA viruses. The insulated isothermal PCR (iiPCR) on a portable POCKIT™ device is user friendly for on-site pathogen detection. In the present study, SVV rRT-PCR and RT-iiPCR were developed and validated. Results Neither the SVV rRT-PCR nor the RT-iiPCR cross-reacted with any of the vesicular disease viruses (20 FMDV, two SVDV, six VSV, and two VESV strains), classical swine fever virus (four strains), and 15 other common swine viruses. Analytical sensitivities of the SVV rRT-PCR and RT-iiPCR were determined using serial dilutions of in vitro transcribed RNA as well as viral RNA extracted from a historical SVV isolate and a contemporary SVV isolate. Diagnostic performances were further evaluated using 125 swine samples by two approaches. First, nucleic acids were extracted from the 125 samples using the MagMAX™ kit and then tested by both RT-PCR methods. One sample was negative by the rRT-PCR but positive by the RT-iiPCR, resulting in a 99.20% agreement (124/125; 95% CI: 96.59–100%, κ = 0.98). Second, the 125 samples were tested by the taco™ mini extraction/RT-iiPCR and by the MagMAX™ extraction/rRT-PCR system in parallel. Two samples were positive by the MagMAX™/rRT-PCR system but negative by the taco™ mini/RT-iiPCR system, resulting in a 98.40% agreement (123/125; 95% CI: 95.39–100%, κ = 0.97). The two samples with discrepant results had relatively high C T values. Conclusions The SVV rRT-PCR and RT-iiPCR developed in this study are very sensitive and specific and have comparable diagnostic performances for SVV RNA detection. The SVV rRT-PCR can be adopted for SVV detection in laboratories. The SVV RT-iiPCR in a simple field-deployable system could serve as a tool to help diagnose vesicular diseases in swine at points of need.

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Section: Introductionmentioning

confidence: 99%

Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

et al. 2019

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“…Furthermore, SVA was recently isolated from neonatal piglets and it is the first report linking SVA with severe neonatal death in America (Canning et al., ). Three reports described detection and genomic characterization of SVA in the United State in 2015 (Guo et al., ; Hause et al., ; Wang et al., ). The Brazilian study evaluated the SVA infection in neonatal piglets and suggested that urine might be a possible route for the dissemination of this virus (Leme et al., ).…”

Section: Discussionmentioning

confidence: 99%

The First Identification and Complete Genome of Senecavirus A Affecting Pig with Idiopathic Vesicular Disease in China

Zhao

Bai

et al. 2016

Transbound Emerg Dis

128

104

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Senecavirus A (SVA) infection was recently confirmed in pigs in Brazil. In March, 2015, an outbreak of vesicular disease occurred in Guangdong, China, characterized by vesicular lesions in sows and acute death of neonatal piglets. Cumulative incidence of porcine idiopathic vesicular disease in farm A was 258, which had a total number of 5500 sows. Sows in farm B displayed typical vesicular symptoms by May, 2015, which also had 5500 sows. A total of 278 and 142 of 5500 sows in farm B demonstrated lame and presented vesicles, respectively, associated with a total of 186 mortality in piglets. Routine differential diagnoses for swine vesicular disease were carried out to exclude infection with foot-and-mouth disease virus, swine vesicular disease virus, vesicular exanthema of swine virus and vesicular stomatitis virus. In this study, seven pairs of primer were designed to amplify the complete genome of SVA in RT-PCR assays. Sequence alignment showed that this Chinese strain shares 94.4-97.1% sequence identity to other eight strains of SVA. This is the first report of SVA in China and provides information about the association between SVA infection and vesicular disease.

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“…Since its first isolation as a contaminant of the PER.C6 cell line in 2001, SVA has been detected in pigs and is associated with idiopathic vesicular disease (IVD). At present, the virus has been reported in Canada, Brazil, the United States and China (Guo et al., ; Hause, Myers, Duff, & Hesse, ; Leme et al., ; Pasma, Davidson, & Shaw, ; Wu et al., ). We herein reported the first detection of SVA in a pig farm in Thailand in 2016.…”

Section: The Studymentioning

confidence: 99%