Novel role of TRPV2 in promoting the cytotoxicity of H2O2-mediated oxidative stress in Human hepatoma cells

Ma, Wenbo; Li, Caiyue; Yin, Shikui; Liu, Jingxin; Gao, Chao; Lin, Zuoxian; Huang, Rongqi; Huang, Jufang; Li, Zhiyuan

doi:10.1016/j.freeradbiomed.2015.09.020

Cited by 26 publications

(23 citation statements)

References 49 publications

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“…[6] Study showed that high level of H 2 O 2 could lead to an abundant phosphorylation of p38 in HepG2 and Huh-7 cell lines. [7] Study also highlighted that phosphorylation of p38 MAPK was significantly enhanced in cells under diabetic conditions. [8] The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) plays a protective role against oxidative stress and inflammation.…”

Section: Introductionmentioning

confidence: 88%

“…The phosphorylation and activation of p38 MAPK not only involve in oxidative stress but also play an important role in glucose utilization . Study showed that high level of H 2 O 2 could lead to an abundant phosphorylation of p38 in HepG2 and Huh‐7 cell lines . Study also highlighted that phosphorylation of p38 MAPK was significantly enhanced in cells under diabetic conditions …”

Section: Introductionmentioning

confidence: 99%

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Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Liu

Tian

et al. 2020

Chemistry & Biodiversity

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Anthocyanins (AC) from Coreopsis tinctoria possesses strong antioxidant properties, while the effects of AC on cells damage induced by reactive oxygen species (ROS) in diabetes mellitus diseases progression have not been reported. The present study was carried out to evaluate the protective property of AC against cellular oxidative stress with an experimental model, H2O2‐exposed MIN6 cells. AC could reverse the decrease of cell viability induced by H2O2 and efficiently suppressed cellular ROS production and cell apoptosis. In addition, Real‐time PCR and Western blot analyses indicated that AC could protect MIN6 cells against oxidative injury through increasing the translocation of Nrf2 into nuclear, decreasing the phosphorylation level of p38 and up‐regulating the protein expression of antioxidant enzyme (SOD1, SOD2 and CAT). Thus, this study provides evidence to support the beneficial effect of AC in inhibiting MIN6 cells from H2O2‐induced oxidative injury.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Liu

Tian

et al. 2020

Chemistry & Biodiversity

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“…Also, TRPV1 and TRPV4 were reported to be involved in modulating cell migration . Our previous study suggested that TRPV2 acts as an important enhancer for H 2 O 2 ‐induced cytotoxicity in HepG2 cells . The fact that upregulation of thermo‐TRPVs in ESCC cells prompted us to test their potential role in the development of ESCC.…”

Section: Discussionmentioning

confidence: 97%

Recurrent activations of transient receptor potential vanilloid‐1 and vanilloid‐4 promote cellular proliferation and migration in esophageal squamous cell carcinoma cells

et al. 2019

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Some members of the transient receptor potential vanilloid (TRPV) subfamily of cation channels are thermosensitive. Earlier studies have revealed the distribution and functions of these thermo‐TRPVs (TRPV1–4) in various organs, but their expression and function in the human esophagus are not fully understood. Here, we probed for the expression of the thermo‐TRPVs in one nontumor human esophageal squamous cell line and two esophageal squamous cell carcinoma (ESCC) cell lines. TRPV1, TRPV2, and TRPV4 proteins were found to be upregulated in ESCC cells, while TRPV3 was not detectable in any of these cell lines. Subsequently, channel function was evaluated via monitoring of Ca2+ transients by Ca2+ imaging and nonselective cation channel currents were recorded by whole‐cell patch clamp. We found that TRPV4 was activated by heat at 28 °C–35 °C, whereas TRPV1 and TRPV2 were activated by higher, noxious temperatures (44 °C and 53 °C, respectively). Furthermore, TRPV1 was activated by capsaicin (EC50 = 20.32 μm), and this effect was antagonized by AMG9810; TRPV2 was activated by a newly developed cannabinoid compound, O1821, and inhibited by tranilast. In addition, TRPV4 was activated by hypotonic solutions (220 m Osm), and this effect was abolished by ruthenium red. The effects of TRPV1 and TRPV4 on ESCC were also explored. Our data, for the first time, showed that the overactivation of TRPV1 and TRPV4 promoted the proliferation and/or migration of ESCC cells. In summary, TRPV1, TRPV2, and TRPV4 were functionally expressed in human esophageal squamous cells, and thermo‐TRPVs might play an important role in the development of ESCC.

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“…Interestingly, the expression of TRPV2 mRNA and protein levels in HepG2 and Huh-7 cells was reported to increase upon exposure to ROS, specifically H 2 O 2 , resulting in an activation of cell death. The elevation of TRPV2 expression led to an inhibition of pro-survival signals (Akt, Nrf2) and, in the early stage of apoptosis, promoted the activation of pro-death signals (p38, JNK1) [85]. Another study showed that shRNA-based TRPV2 knockdown in HepG2 cells enhanced spheroid and colony formation, which was restored by the overexpression of TRPV2.…”

Section: Liver Cancermentioning

confidence: 98%

TRP Channels in Digestive Tract Cancers

Stokłosa

Borgström

Kappel

et al. 2020

IJMS

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Cancers of the digestive tract are among the most prevalent types of cancer. These types of cancers are often diagnosed at a late stage, which results in a poor prognosis. Currently, many biomedical studies focus on the role of ion channels, in particular transient receptor potential (TRP) channels, in cancer pathophysiology. TRP channels show mostly non-selective permeability to monovalent and divalent cations. TRP channels are often dysregulated in digestive tract cancers, which can result in alterations of cancer hallmark functions, such as enhanced proliferation, migration, invasion and the inability to induce apoptosis. Therefore, TRP channels could serve as potential diagnostic biomarkers. Moreover, TRP channels are mostly expressed on the cell surface and ion channel targeting drugs do not need to enter the cell, making them attractive candidate drug targets. In this review, we summarize the current knowledge about TRP channels in connection to digestive tract cancers (oral cancer, esophageal cancer, liver cancer, pancreatic cancer, gastric cancer and colorectal cancer) and give an outlook on the potential of TRP channels as cancer biomarkers or therapeutic targets.

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Novel role of TRPV2 in promoting the cytotoxicity of H2O2-mediated oxidative stress in Human hepatoma cells

Cited by 26 publications

References 49 publications

Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Protective Effects of Anthocyanins from Coreopsis tinctoria against Oxidative Stress Induced by Hydrogen Peroxide in MIN6 Cells

Recurrent activations of transient receptor potential vanilloid‐1 and vanilloid‐4 promote cellular proliferation and migration in esophageal squamous cell carcinoma cells

TRP Channels in Digestive Tract Cancers

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