Novel RNA viruses from the native range of Hymenoscyphus fraxineus, the causal fungal agent of ash dieback

Shamsi, Wajeeha; Kondō, Hideki; Ulrich, Sven; Rigling, Daniel; Prospero, Simone

doi:10.1016/j.virusres.2022.198901

Cited by 11 publications

(9 citation statements)

References 89 publications

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“…In parallel, the native range of H. fraxineus in eastern Asia was identified (Zhao et al, 2013;Drenkhan et al, 2017), and its pathogenicity in its natural environment was investigated (Cleary et al, 2016). These early research directions have yielded fundamental information on the pathogen, hosts, and disease cycle that have stimulated further research to cope with ash dieback, such as the search for mycoviruses as biological control of H. fraxineus (e.g., Schoebel et al, 2014;Shamsi et al, 2022). At present, whether the use of mycoviruses can be successfully used as biocontrol of H. fraxineus remains unclear because this method relies strongly on the spread and hypovirulence of mycoviruses within H. fraxineus populations, which the fungal host population may hinder (Gross et al, 2014b;Burokiene et al, 2015).…”

Section: Ash Dieback In Europe: Impacts and Research Directionsmentioning

confidence: 99%

“…At present, whether the use of mycoviruses can be successfully used as biocontrol of H. fraxineus remains unclear because this method relies strongly on the spread and hypovirulence of mycoviruses within H. fraxineus populations, which the fungal host population may hinder (Gross et al, 2014b;Burokiene et al, 2015). Further work investigating virus-mediated controls of ash dieback in Europe is thus necessary (Shamsi et al, 2022) before using mycoviruses for biocontrol of ash dieback. More recently, studies have investigated the potential of fungal endophytes for biocontrol of ash dieback (e.g., Schlegel et al, 2016;Kosawang et al, 2018;Halecker et al, 2020).…”

Section: Ash Dieback In Europe: Impacts and Research Directionsmentioning

confidence: 99%

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Potential alternative tree species to Fraxinus excelsior in European forests

Lévesque

Eduardo

Queloz

2023

Front. For. Glob. Change

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Common ash (Fraxinus excelsior L.) is a keystone tree species in Europe. However, since the 1990s, this species has been experiencing widespread decline and mortality due to ash dieback [Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz and Hosoya]. Besides H. fraxineus, emerald ash borer (Agrilus planipennis Fairmaire), an invasive alien pest already devastating ash trees in western Russia, is spreading westward and becoming an emerging threat to the remaining European ash populations. While efforts to control ash dieback continue to be a priority, it is becoming crucial to compensate for the loss of ash and its ecosystem services by elaborating restoration strategies, including the search for alternative native and non-native tree species. This review summarizes available knowledge on potential alternative tree species to common ash to help forest managers to cope with ash dieback. Although using natural regeneration and promoting tree species diversity can reduce the impacts of ash dieback in European forests, our review indicates that no native species alone or in combination can fully replace the ecological niche of common ash and its associated ecosystem services (e.g., biodiversity and timber). To fill this gap, forest managers have considered using non-native ash species that are tolerant to both H. fraxineus and A. planipennis and have similar ecological and forestry values as common ash. Of the 43 ash species reviewed, few non-native ash species (e.g., Fraxinus mandshurica Rupr. and Fraxinus platypoda Oliv.) have similar ecological characteristics to common ash and are tolerant to H. fraxineus and A. planipennis. However, the performance of non-native ash species in European forests, their invasiveness potential, and the risk of hybridization with native ash species are still unknown. With the current state of knowledge, it is thus too early to recommend the use of non-native ash species as a suitable option to deal with ash dieback. The priority should be the conservation, regeneration, and breeding of tolerant common ash populations to H. fraxineus, as well as the use of the natural regeneration of other native tree species. Our review highlights the need for controlled experimental plantations to better understand the regeneration ecology and invasiveness potential of non-native ash species prior to their utilization in natural forests.

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Section: Ash Dieback In Europe: Impacts and Research Directionsmentioning

confidence: 99%

Section: Ash Dieback In Europe: Impacts and Research Directionsmentioning

confidence: 99%

Potential alternative tree species to Fraxinus excelsior in European forests

Lévesque

Eduardo

Queloz

2023

Front. For. Glob. Change

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“…In total, 105 isolates of H. fraxineus from two Japanese populations were used, which were screened for mycoviruses in a previous study (Shamsi et al, 2022). One isolate (R1153) was found to be infected with the mitovirus (HfMV2), while the rest of the isolates were HfMV2-free.…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…In the present study, we investigated the potential of a second mitovirus (Hymenoscyphus fraxineus mitovirus 2, HfMV2) recently identified in the native population of H. fraxineus (Shamsi et al, 2022), to be used as a biocontrol agent against ash dieback. Indeed, specialized and co-evolved enemies (including hyperparasites) are more likely to occur in the native range of a pathogen than in the introduced range.…”

Section: Introductionmentioning

confidence: 99%

Possible biological control of ash dieback using the parasitic Hymenoscyphus fraxineus mitovirus 2?

Shamsi

Mittelstrass

Kondō

et al. 2023

Preprint

Self Cite

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Invasive fungal diseases represent a major threat to forest ecosystems worldwide. As fungicides are often unfeasible and not a sustainable solution, only a few other control options are available, including biological control. In this context, the use of parasitic mycoviruses as biocontrol agents of fungal pathogens has recently gained particular attention. Since the 1990s, the Asian fungusHymenoscyphus fraxineushas been causing lethal ash dieback across Europe. In the present study, we investigated the biocontrol potential of the mitovirus Hymenoscyphus fraxineus mitovirus 2 (HfMV2) previously identified in Japanese populations of the pathogen. HfMV2 could be successfully introduced via co-culturing into 16 out of 105 virus-free isolates. A virus infection had contrasting effects on fungal growthin vitro, from cryptic to detrimental or beneficial. Virus-infectedH. fraxineusisolates whose growth was reduced by HfMV2 showed a lower virulence on ash (Fraxinus excelsior) saplings compared to their isogenic virus-free isolates. The results suggest that mycoviruses exist in the native populations ofH. fraxineusin Asia that have the potential for biological control of ash dieback in Europe.

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“…Viruses with genomic similarities to bunyaviruses have more recently also been described from fungi and oomycetes. Fugal species hosting these unclassified bunya-like viruses include several potential phytopathogens [38][39][40][41][42] as well as plant endophytes [43]. Further hosts include the shiitake mushroom (Lentinula edodes) [44] and a marine fungus, Penicillium roseopurpureum [45].…”

Section: Introductionmentioning

confidence: 99%

Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

Poimala

Raco

Haikonen

et al. 2022

Viruses

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Phytophthora cactorum is an important oomycetous plant pathogen with numerous host plant species, including garden strawberry (Fragaria × ananassa) and silver birch (Betula pendula). P. cactorum also hosts mycoviruses, but their phenotypic effects on the host oomycete have not been studied earlier. In the present study, we tested polyethylene glycol (PEG)-induced water stress for virus curing and created an isogenic virus-free isolate for testing viral effects in pair with the original isolate. Phytophthora cactorum bunya-like viruses 1 and 2 (PcBV1 & 2) significantly reduced hyphal growth of the P. cactorum host isolate, as well as sporangia production and size. Transcriptomic and proteomic analyses revealed an increase in the production of elicitins due to bunyavirus infection. However, the presence of bunyaviruses did not seem to alter the pathogenicity of P. cactorum. Virus transmission through anastomosis was unsuccessful in vitro.

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Novel RNA viruses from the native range of Hymenoscyphus fraxineus, the causal fungal agent of ash dieback

Cited by 11 publications

References 89 publications

Potential alternative tree species to Fraxinus excelsior in European forests

Potential alternative tree species to Fraxinus excelsior in European forests

Possible biological control of ash dieback using the parasitic Hymenoscyphus fraxineus mitovirus 2?

Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

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