Novel Reassortant Influenza A(H5N8) Viruses in Domestic Ducks, Eastern China

Wu, Haibo; Peng, Xiaorong; Xu, Lixin; Jin, Changzhong; Cheng, Linfang; Lu, Xiangyun; Xie, Tiansheng; Yao, Hang‐Ping; Wu, Nanping

doi:10.3201/eid2008.140339

Cited by 136 publications

(124 citation statements)

References 15 publications

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“…Zhejiang province is located in the south-east coastal area of China and has been an epicentre for the emergence of AIVs of public health importance in recent years, including the highly pathogenic H7N9 viruses (Wu et al, 2014(Wu et al, , 2015Lam et al, 2015;Yu et al, 2015a). In contrast, Wuhan is the capital of Hubei province located in central China.…”

Section: Introductionmentioning

confidence: 99%

Diversity and evolution of avian influenza viruses in live poultry markets, free-range poultry and wild wetland birds in China

Chen

Lin

Guo

et al. 2016

Journal of General Virology

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The wide circulation of novel avian influenza viruses (AIVs) highlights the risk of pandemic influenza emergence in China. To investigate the prevalence and genetic diversity of AIVs in different ecological contexts, we surveyed AIVs in live poultry markets (LPMs), free-range poultry and the wetland habitats of wild birds in Zhejiang and Hubei provinces. Notably, LPMs contained the highest frequency of AIV infection, and the greatest number of subtypes (n59) and subtype co-infections (n514), as well as frequent reassortment, suggesting that they play an active role in fuelling AIV transmission. AIV-positive samples were also identified in wild birds in both provinces and free-range poultry in one sampling site close to a wetland region in Hubei. H9N2, H7N9 and H5N1 were the most commonly sampled subtypes in the LPMs from Zhejiang, whilst H5N6 and H9N2 were the dominant subtypes in the LPMs from Hubei. Phylogenetic analyses of the whole-genome sequences of 43 AIVs revealed that three reassortant H5 subtypes were circulating in LMPs in both geographical regions. Notably, the viruses sampled from the wetland regions and free-range poultry contained complex reassortants, for which the origins of some segments were unclear. Overall, our study highlights the extent of AIV genetic diversity in two highly populated parts of central and south-eastern China, particularly in LPMs, and emphasizes the need for continual surveillance.

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Section: Introductionmentioning

confidence: 99%

Diversity and evolution of avian influenza viruses in live poultry markets, free-range poultry and wild wetland birds in China

Chen

Lin

Guo

et al. 2016

Journal of General Virology

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“…In particular, H5N5, H5N8 and H5N2 subtypes bearing the genetic backbone of clade 2.3.4 H5N1 viruses have been identified in poultry populations (Gu et al, 2011;Zhao et al, 2012;Wu et al, 2014).…”

mentioning

confidence: 99%

Two novel reassortants of avian influenza A (H5N6) virus in China

Shi

et al. 2015

Journal of General Virology

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“…H5N8 HPAI virus, which has hemagglutinin protein derived from H5N1 HPAI virus, is also currently epidemic and produces severe clinical signs to ducks. 21,50 Because hemagglutinin protein of avian influenza virus is a critical factor for its virulence, 32 clinical signs-such as neurologic signs and corneal opacity, which are similar to those caused by H5N1 HPAI virus-may be found in domestic ducks infected with H5N8 HPAI virus.…”

Section: Discussionmentioning

confidence: 99%

“…were established for inoculation with Ws/Akita/1/08. The domestic ducks in each group were intranasally inoculated with 10 1 to 10 6 50% egg infectious doses (EID 50 ) of the corresponding virus. Clinical findings, including mortality, depression, neurologic signs, and ocular changes, were recorded daily for 14 days.…”

Section: Experimental Infectionmentioning

confidence: 99%

Corneal Opacity in Domestic Ducks Experimentally Infected With H5N1 Highly Pathogenic Avian Influenza Virus

et al. 2015

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Domestic ducks can be a key factor in the regional spread of H5N1 highly pathogenic avian influenza (HPAI) virus in Asia. The authors performed experimental infections to examine the relationship between corneal opacity and H5N1 HPAI virus infection in domestic ducks (Anas platyrhyncha var domestica). A total of 99 domestic ducks, including 3 control birds, were used in the study. In experiment 1, when domestic ducks were inoculated intranasally with 2 H5N1 HPAI viruses, corneal opacity appeared more frequently than neurologic signs and mortality. Corneal ulceration and exophthalmos were rare findings. Histopathologic examinations of the eyes of domestic ducks in experiment 2 revealed that corneal opacity was due to the loss of corneal endothelial cells and subsequent keratitis with edema. Influenza viral antigen was detected in corneal endothelial cells and some other ocular cells by immunohistochemistry. Results suggest that corneal opacity is a characteristic and frequent finding in domestic ducks infected with the H5N1 HPAI virus. Confirming this ocular change may improve the detection rate of infected domestic ducks in the field.

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Novel Reassortant Influenza A(H5N8) Viruses in Domestic Ducks, Eastern China

Cited by 136 publications

References 15 publications

Diversity and evolution of avian influenza viruses in live poultry markets, free-range poultry and wild wetland birds in China

Diversity and evolution of avian influenza viruses in live poultry markets, free-range poultry and wild wetland birds in China

Two novel reassortants of avian influenza A (H5N6) virus in China

Corneal Opacity in Domestic Ducks Experimentally Infected With H5N1 Highly Pathogenic Avian Influenza Virus

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