Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH)

Packard, Michelle M.; Shusteff, Maxim; Alocilja, Evangelyn C.

doi:10.1117/12.892425

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…Over the last 2 decades, much effort has focused on resolving this issue by development of sample pretreatment methods that can be integrated with biosensors. These approaches include microfluidic separation (Zuo and others ; Packard and others ; Clime and others ; Kim and others ; Lee and others ; Luka and others ; Gashti and others ), nanoparticle separation (Varshney and Li ; Kwon and others ; Wang and others ), magnetic relaxation switching (Chen and others ), dielectrophoresis (Cheng and others ; Packard and others ; Wang and others ; Yang ; Hamada and others ; Couniot and others ; Kim and others ; Fernandez and others ), immunochromatography (Vyas and others ), acoustofluidic sorting (Li and others ), ferrofluidic manipulation (Kose and others ), or hydrodynamic focusing (Clime and others ). Although not reviewed in detail here, sample pretreatment is a major focus of biosensor research labs and the primary challenges are minimizing destructive sampling, need for pumps, energy, and use of exogenous chemicals.…”

Section: Introductionmentioning

confidence: 99%

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

Vanegas

Gomes

Cavallaro

et al. 2017

Comp Rev Food Sci Food Safe

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The presence of unsafe levels of microorganisms in food constitutes a growing economic and public health problem that necessitates new technology for their rapid detection along the food continuum from production to consumption. While traditional techniques are reliable, there is a need for more sensitive, selective, rapid, and costeffective approaches for food safety evaluation. Methods such as microbiological counts are sufficiently accurate and inexpensive, and are capable of determining presence and viability for most pathogens. However, these techniques are time consuming, involve destructive sampling, and require trained personnel and biosafety-certified facilities for analysis. Molecular techniques such as the polymerase chain reaction have greatly improved analytical capability over the last decade, achieving shorter analysis time with quantitative data and strain specificity, and in some cases the ability to discriminate cell viability. The emerging field of nanosensors/biosensors has demonstrated a variety of devices that hold promise to bridge the gap between traditional plate counting and molecular techniques. Many nanosensors/biosensors are rapid, portable, accurate devices that can be used as an additional screening tool for identifying unsafe levels of microorganisms in food products with no need for pre-enrichment. In this review, we provide a brief overview of available biorecognitiontransduction techniques for detecting bacteria in food. We then discuss the advantages and disadvantages of each technique, and describe some recent biosensor or nanosensor technologies that are under development. We conclude by summarizing the opportunities and challenges in the field of pathogen monitoring in food systems and we focus the discussion on the strengths/weaknesses of the most popular biorecognition agents and transducer nanomaterials for biosensing.

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Section: Introductionmentioning

confidence: 99%

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

Vanegas

Gomes

Cavallaro

et al. 2017

Comp Rev Food Sci Food Safe

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Micro fluorescence in situ hybridization (μFISH) for spatially multiplexed analysis of a cell monolayer

Huber

Autebert

Kaigala

2016

Biomed Microdevices

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We here present a micrometer-scale implementation of fluorescence in situ hybridization that we term μFISH. This μFISH implementation makes use of a non-contact scanning probe technology, namely, a microfluidic probe (MFP) that hydrodynamically shapes nanoliter volumes of liquid on a surface with micrometer resolution. By confining FISH probes at the tip of this microfabricated scanning probe, we locally exposed approximately 1000 selected MCF-7 cells of a monolayer to perform incubation of probes — the rate-limiting step in conventional FISH. This method is compatible with the standard workflow of conventional FISH, allows re-budgeting of the sample for various tests, and results in a ~ 15-fold reduction in probe consumption. The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. We further demonstrated spatially multiplexed μFISH, enabling the use of spectrally equivalent probes for detailed and real-time analysis of a cell monolayer, which paves the way towards rapid and automated multiplexed FISH on standard cytological supports.Electronic supplementary materialThe online version of this article (doi:10.1007/s10544-016-0064-0) contains supplementary material, which is available to authorized users.

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Front Matter: Volume 9930

Mohseni¹,

Agahi²,

Razeghi³

2016

SPIE Proceedings

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Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH)

Cited by 3 publications

References 6 publications

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

Emerging Biorecognition and Transduction Schemes for Rapid Detection of Pathogenic Bacteria in Food

Micro fluorescence in situ hybridization (μFISH) for spatially multiplexed analysis of a cell monolayer

Front Matter: Volume 9930

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