Novel prokaryotic diversity in sediments of Tunisian multipond solar saltern

Baati, Houda; Guermazi, Sonda; Gharsallah, Néji; Sghir, Abdelghani; Ammar, Emna

doi:10.1016/j.resmic.2010.05.009

Cited by 46 publications

(27 citation statements)

References 38 publications

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“…Deltaproteobacterium OTU (PB-86) showed only modest similarity (96%) with an environmental clone found in the Guerrero Negro solar salterns [22] and was not closely related to cultured members of this subphylum (>15% similar to cultured Deltaproteobacterial species). Recovering of sulphate-reducing lineages from Deltaproteobacterium has been already reported for hypersaline environments [22,33].…”

Section: Salt Composition Of Watermentioning

confidence: 79%

Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

Kambourova

Tomova

Boyadzhieva

et al. 2016

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the bacterial community habituating P18, the biggest crystallizer pond in Pomorie salterns (34% salinity). The obtained results showed that the bacterial community differs from many previous reports of low bacterial diversity in hypersaline environments and demonstrates unusually high diversity of presented taxa, some unusual domination of diverse genera not reported before as dominant and identification of previously unknown 16S rRNA sequences. The retrieved 23 bacterial operational taxonomic units (OTUs) affiliated with 15 bacterial genera from four phyla -Firmicutes, 47.5%; Proteobacteria, 23.1%; Bacteroidetes, 22%; Deinococcus-Thermus, 2.4%; and one-candidate division SR1, 4.8%. Representatives of the phylum Firmicutes predominated in the bacterial community with almost half of the retrieved sequences. Almost all clones branched together with cultured halophiles or uncultured clones retrieved from saline niches. Despite of the high salt concentration, some of the closest phylogenetic neighbours were moderate halophiles. New sequences represented 42.3% of bacterial OTUs. Some of them formed separate branches with similarity less than 85%.

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Section: Salt Composition Of Watermentioning

confidence: 79%

Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

Kambourova

Tomova

Boyadzhieva

et al. 2016

Biotechnology & Biotechnological Equipment

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“…Aside from a clear separation from the monimolimnion assemblages, the communities also showed a vertical variation. As the samples from 0.5 and 2 m depths were mainly dominated by Halobacteriaceae reads, related to an environmentally derived DNA sequence from a solar saltern (Baati et al, 2010), the 3 m depth sample contained a high abundance of reads belonging to the MSP41 group (~47.7%). Moreover, a V4 SSU rDNA phylogenetic tree suggested that the 3 m community consists of OTUs related to candidate genera Nanosalinarum, Nanosalina and Haloredivivus (Supplementary Figure S3) (Ghai et al, 2011;Narasingarao et al, 2012).…”

Section: Methanogenesismentioning

confidence: 99%

Contrasting taxonomic stratification of microbial communities in two hypersaline meromictic lakes

et al. 2015

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Hypersaline meromictic lakes are extreme environments in which water stratification is associated with powerful physicochemical gradients and high salt concentrations. Furthermore, their physical stability coupled with vertical water column partitioning makes them important research model systems in microbial niche differentiation and biogeochemical cycling. Here, we compare the prokaryotic assemblages from Ursu and Fara Fund hypersaline meromictic lakes (Transylvanian Basin, Romania) in relation to their limnological factors and infer their role in elemental cycling by matching taxa to known taxon-specific biogeochemical functions. To assess the composition and structure of prokaryotic communities and the environmental factors that structure them, deepcoverage small subunit (SSU) ribosomal RNA (rDNA) amplicon sequencing, community domainspecific quantitative PCR and physicochemical analyses were performed on samples collected along depth profiles. The analyses showed that the lakes harbored multiple and diverse prokaryotic communities whose distribution mirrored the water stratification patterns. Ursu Lake was found to be dominated by Bacteria and to have a greater prokaryotic diversity than Fara Fund Lake that harbored an increased cell density and was populated mostly by Archaea within oxic strata. In spite of their contrasting diversity, the microbial populations indigenous to each lake pointed to similar physiological functions within carbon degradation and sulfate reduction. Furthermore, the taxonomy results coupled with methane detection and its stable C isotope composition indicated the presence of a yet-undescribed methanogenic group in the lakes' hypersaline monimolimnion. In addition, ultrasmall uncultivated archaeal lineages were detected in the chemocline of Fara Fund Lake, where the recently proposed Nanohaloarchaeota phylum was found to thrive.

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“…The phylogenetic diversity and community structure of members of the Halobacteriales have been investigated in various thalassohaline (e.g., crystallizer ponds in solar salterns [3,42,45]) and athalasohaline (e.g., the Dead Sea and soda lakes [46]) water bodies. In such hypersaline habitats (Ͼ25% NaCl), members of the Halobacteriales often represent the dominant microbial fraction observed, and hence their diversity could readily be examined using general archaeal 16S rRNA gene primers.…”

mentioning

confidence: 99%

“…In such hypersaline habitats (Ͼ25% NaCl), members of the Halobacteriales often represent the dominant microbial fraction observed, and hence their diversity could readily be examined using general archaeal 16S rRNA gene primers. Collectively, these studies have indicated that while low overlap between communities in seemingly similar habitats (e.g., solar salterns) is often observed, Halobacteriales diversity in such habitats is often limited (3,8,45), with a single or few phylotypes often representing the majority of the Halobacteriales community within such ecosystems. Further, while 16S rRNA gene sequences belonging to putatively novel Halobacteriales genera have been identified in some of these studies (see, e.g., reference 45), the majority of halophilic Archaea identified in such habitats belong to various wellcharacterized Halobacteriales genera, e.g., Halorubrum, Haloferax, and Haloquadratum (3,8,45).…”

mentioning

confidence: 99%

Phylogenetic Diversities and Community Structure of Members of the Extremely Halophilic Archaea (Order Halobacteriales) in Multiple Saline Sediment Habitats

Youssef

Ashlock-Savage

2012

Appl Environ Microbiol

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We investigated the phylogenetic diversity and community structure of members of the halophilic Archaea (order Halobacteriales) in five distinct sediment habitats that experience various levels of salinity and salinity fluctuations (sediments from Great Salt Plains and Zodletone Spring in Oklahoma, mangrove tree sediments in Puerto Rico, sediment underneath salt heaps in a salt-processing plant, and sediments from the Great Salt Lake northern arm) using Halobacteriales-specific 16S rRNA gene primers. Extremely diverse Halobacteriales communities were encountered in all habitats, with 27 (Zodletone) to 37 (mangrove) different genera identified per sample, out of the currently described 38 Halobacteriales genera. With the exception of Zodletone Spring, where the prevalent geochemical conditions are extremely inhospitable to Halobacteriales survival, habitats with fluctuating salinity levels were more diverse than permanently saline habitats. Sequences affiliated with the recently described genera Halogranum, Halolamina, Haloplanus, Halosarcina, and Halorientalis, in addition to the genera Halorubrum, Haloferax, and Halobacterium, were among the most abundant and ubiquitous genera, suggesting a wide distribution of these poorly studied genera in saline sediments. The Halobacteriales sediment communities analyzed in this study were more diverse than and completely distinct from communities from typical hypersaline water bodies. Finally, sequences unaffiliated with currently described genera represented a small fraction of the total Halobacteriales communities, ranging between 2.5% (Zodletone) to 7.0% (mangrove and Great Salt Lake). However, these novel sequences were characterized by remarkably high levels of alpha and beta diversities, suggesting the presence of an enormous, yet-untapped supply of novel Halobacteriales genera within the rare biosphere of various saline ecosystems. Members of the halophilic Archaea (order Halobacteriales) are a physiologically and phylogenetically distinct group of microorganisms, characterized by their obligate halophilic lifestyle, aerobic heterotrophic metabolism, and characteristic red coloration (48). The development of red coloration in hypersaline brines is a phenomenon that was observed thousands of years ago (50). Studies in the early 20th century identified the cause of this coloration and described multiple halophilic archaeal isolates (50). The realization that these organisms are members of the Archaea came in 1978 (41). Since then, various Halobacteriales species have been used as model microorganisms for understanding physiological and structural adaptations to living in hypersaline conditions and for understanding basic cellular processes in Archaea (33).The phylogenetic diversity and community structure of members of the Halobacteriales have been investigated in various thalassohaline (e.g., crystallizer ponds in solar salterns [3,42,45]) and athalasohaline (e.g., the Dead Sea and soda lakes [46]) water bodies. In such hypersaline habitats (Ͼ25% NaCl), members of th...

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Novel prokaryotic diversity in sediments of Tunisian multipond solar saltern

Cited by 46 publications

References 38 publications

Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

Phylogenetic analysis of the bacterial community in a crystallizer pond, Pomorie salterns, Bulgaria

Contrasting taxonomic stratification of microbial communities in two hypersaline meromictic lakes

Phylogenetic Diversities and Community Structure of Members of the Extremely Halophilic Archaea (Order Halobacteriales) in Multiple Saline Sediment Habitats

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