Novel porous carbon packings in reversed-phase high-performance liquid chromatography

Unger, Klaus K.; Roumeliotis, P.; Mueller, H.; Goetz, Hermann

doi:10.1016/s0021-9673(00)80071-0

Cited by 45 publications

(22 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One potential drawback in using core shell particles might be relatively lower sample loading capacity compared to totally porous materials due to their reduced surface area. In addition, several reports indicated that carbon packings have rather low loading capacity [45,46]. Thus, we estimated the loading capacity of a column packed with the PSH C/Al/SiO 2 and compared it with other packing materials.…”

Section: Resultsmentioning

confidence: 99%

Development of a carbon clad core-shell silica for high speed two-dimensional liquid chromatography

Paek

Huang

Filgueira

et al. 2012

Journal of Chromatography A

View full text Add to dashboard Cite

We recently introduced a new method ([1]) to deposit carbon on fully porous silicas (5 μm) to address some of the shortcomings of carbon clad zirconia (C/ZrO2), which has rather low retention due to its low surface area (20–30 m2/g). The method enables the introduction of a thin, homogeneous layer of Al (III) on silica to serve as catalytic sites for carbon deposition without damaging the silica’s native pore structure. Subsequent carbon deposition by chemical vapor deposition resulted in chromatographically useful carbon phases as shown by good efficiencies and higher retentivity relative to C/ZrO2. Herein, we use the above method to develop a novel carbon phase on superficially porous silica (2.7 μm). This small, new form of silica offers better mass transfer properties and higher efficiency with lower column back pressures as compared to sub 2 μm silica packings, which should make it attractive for use as the second dimension in fast two-dimensional LC (LC x LC). After carbon deposition, several studies were conducted to compare the new packing with C/ZrO2. Consistent with work on 5 μm fully porous silica, the metal cladding did not cause pore blockage. Subsequent carbon deposition maintained the good mass transfer properties as shown by the effect of velocity on HETP. The new packing exhibits efficiencies up to ~ 5.6 fold higher than C/ZrO2 for polar compounds. We observed similar chromatographic selectivity for all carbon phases tested. Consequently, the use of the new packing as the second dimension in fast LC x LC improved the peak capacity of fast LC x LC. The new material gave loading capacities similar to C/ZrO2, which is rather expected based on the surface areas of the two phases.

show abstract

Section: Resultsmentioning

confidence: 99%

Development of a carbon clad core-shell silica for high speed two-dimensional liquid chromatography

Paek

Huang

Filgueira

et al. 2012

Journal of Chromatography A

View full text Add to dashboard Cite

show abstract

“…Unger et al (23,24) employed suffi ciently hard cokes and active carbons as precursors. The material was milled, sized into narrow fractions, and then subjected to various extrac tions and heat treatments to achieve carbonization and removal of noncarbonaceous constituents.…”

Section: Preparation Of Carbons For Hplcmentioning

confidence: 99%

“…Microparticulate carbons narrowly distributed in the 5-20-μηι range are packed by the usual slurry technique; the slurry liquid composition is adapt ed to the wetting behavior of carbon (7,24,27). Packing stability studies on carbon columns indicate that pres sures above 800 bar at corresponding ly high flow rates may be applied without bringing about any change of column bed and particle geometry (28).…”

Section: Chromatography On Carbon Columnsmentioning

confidence: 99%

“…Column loadability has been the subject of thorough investigation (5,24). When normalized to unit surface area of packing, and assuming the col umn porosity to be equal, the linear sample capacity 0n.i [following Sny der's definition (30)] is found to be on the same order for carbon and re versed-phase silicas (9).…”

Section: Chromatography On Carbon Columnsmentioning

confidence: 99%

“…When normalized to unit surface area of packing, and assuming the col umn porosity to be equal, the linear sample capacity 0n.i [following Sny der's definition (30)] is found to be on the same order for carbon and re versed-phase silicas (9). Values com parable to 00.1 are obtained (5,24) when 00.5 is employed, where #o.5 is the mass of solute per gram of packing that produces a 50% reduction in col umn efficiency. When deviations be tween 0o.i and 0o.s arise, they are de pendent on the type of eluent and sol utes.…”

Section: Chromatography On Carbon Columnsmentioning

confidence: 99%

See 2 more Smart Citations