Novel porous aortic elastin and collagen scaffolds for tissue engineering

Mechanical characterization of a customized decellularized scaffold for vascular tissue engineering

“…Axial perforation of the decellularized tissue with 50µm holes using a laser noted a higher level of cell repopulation in vivo. Pure collagen and pure elastin scaffolds have been studied (Chuang et al, 2009, Simionescu et al, 2006, Lu et al, 2004, Kurane et al, 2007. Completely removing these individual ECM components noted a higher infiltration of cells in the elastin scaffolds in-vivo.…”

“…A major drawback, however, is that the matrix that remains after decellularization is extremely dense and can prove difficult to infiltrate with cells. This is particularly the case with SMCs in the medial layer of the construct (Lu et al, 2004, Neff et al, 2010 . A poorly infiltrated scaffold resulting in a lack of a fully quiescent contractile SMC medial layer has resulted in less than ideal mechanical properties and diminished performance in vivo (Yazdani et al, 2009).…”

Osteogenesis and angiogenesis: The potential for engineering bone

“…Natural polymers such as collagen are of a major interest because they are biodegradable and biocompatable, allowing cells to adhere, proliferate and differentiate without the problems of inflammatory reactions and cytotoxicity which are often associated with synthetic polymers. (Lu et al, 2004;Lubiatowski et al, 2006;Domaschke et al, 2006). However, natural polymers are structurally complex compared to synthetic polymers therefore, technical manipulation is complicated and more elaborate causing Osteogenesis, angiogenesis and bone tissue engineering variation in the processing of consistent scaffold matrices.…”

Assembly and Testing of Stem Cell-Seeded Layered Collagen Constructs for Heart Valve Tissue Engineering

“…To detect Gala we performed lectin histochemistry using biotinylated Griffonia simplicifolia lectin as the primary reactant, followed by ABC-peroxidase complex and diaminobenzidine (DAB) detection with hematoxylin counterstaining. 26 Scanning electron microscopy sample preparation was performed according to standard procedures 35 and imaged on a Hitachi S4800. To test for cytocompatibility, rat dermal fibroblasts were seeded (10 5 cells/ cm 2 ) onto sterile scaffolds, and cell viability and proliferation evaluated weekly for up to 5 weeks using LIVE/DEAD Ò stain (Molecular Probes) and CellTiter 96 Ò AQueous One Solution Cell Proliferation Assay [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) (Promega) as per manufacturers' directions.…”