Novel phenotypes related to the breeding of purple-fruited tomatoes and effect of peel extracts on human cancer cell proliferation

Mazzucato, Andrea; Willems, Daniela; Bernini, Roberta; Picarella, Maurizio Enea; Santangelo, Enrico; Ruiu, Fabrizio; Tilesi, Francesca; Soressi, G. P.

doi:10.1016/j.plaphy.2013.05.012

Cited by 50 publications

(36 citation statements)

References 33 publications

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“…An excellent antioxidant capacity has been observed in the plasma of rats (p < 0.01) [63] and in the serum of humans (p < 0.05) [64] with increased intakes of anthocyanins. Furthermore, treatment with anthocyanins extracted from purple-tomato peel at 52.5 µg/mL showed reduction in ovarian cancer cell lines (p < 0.05) [65]. Provided that the total anthocyanins contents in the extracts of Laird's Large peel and Mulligan peel were 87 and 144 µg/mL, respectively ( Table 1; unit converted to µg/mL), tamarillo peels are likely to show similar extents of antioxidant activity.…”

Section: Discussionmentioning

confidence: 93%

Phenolic and Anthocyanin Compounds and Antioxidant Activity of Tamarillo (Solanum betaceum Cav.)

2020

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This study examined phenolics and anthocyanins present in Amber, Laird’s Large and Mulligan cultivars of tamarillo that were cultivated in Whangarei, Northland of New Zealand. Samples were further separated by their tissue types, peel and pulp. Using LC-MS/MS, twelve polyphenols were quantified and six (ellagic acid, rutin, catechin, epicatechin, kaempferol-3-rutinoside and isorhamnetin-3-rutinoside) were detected for the first time in tamarillo. Mulligan cultivar showed the highest amounts of phenolic and anthocyanin compounds and the highest antioxidant activity. Phenolic compounds were mostly synthesized from shikimic acid route, and chlorogenic acid dominated the profile regardless of cultivar and tissue types. Anthocyanin profile was dominated by delphinidin-3-rutinoside in pulp. Higher amounts of anthocyanins were detected in this study, which may be explained by favourable growth conditions (high light intensity and low temperature) for anthocyanin biosynthesis in New Zealand. Higher antioxidant activity and total phenolic content in peels than in pulps were found when assessed by Cupric Ion-Reducing Antioxidant Capacity (CUPRAC), Ferric Reducing Ability of Plasma (FRAP) and Folin–Ciocalteu assays, and a positive correlation (r > 0.9, p ≤ 0.01) between the three assays was observed. Current findings endorse that tamarillo has a great bioactive potential to be developed further as a functional ingredient with considerable levels of antioxidant compounds and antioxidant activity.

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Section: Discussionmentioning

confidence: 93%

Phenolic and Anthocyanin Compounds and Antioxidant Activity of Tamarillo (Solanum betaceum Cav.)

2020

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“…Mazzucato et al . [67] provided evidence that higher anthocyanin content is associated with increased early fruit growth in non-pollinated flowers. Furthermore, there is an evidence that the alteration of the flavonoid pathway via the regulation of biosynthesis genes induces seedless fruit development in both a pollination-dependent and pollination-independent manner [68,69].…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification of pistil-specific genes expressed during fruit set initiation in tomato (Solanum lycopersicum)

et al. 2017

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Fruit set involves the developmental transition of an unfertilized quiescent ovary in the pistil into a fruit. While fruit set is known to involve the activation of signals (including various plant hormones) in the ovary, many biological aspects of this process remain elusive. To further expand our understanding of this process, we identified genes that are specifically expressed in tomato (Solanum lycopersicum L.) pistils during fruit set through comprehensive RNA-seq-based transcriptome analysis using 17 different tissues including pistils at six different developmental stages. First, we identified 532 candidate genes that are preferentially expressed in the pistil based on their tissue-specific expression profiles. Next, we compared our RNA-seq data with publically available transcriptome data, further refining the candidate genes that are specifically expressed within the pistil. As a result, 108 pistil-specific genes were identified, including several transcription factor genes that function in reproductive development. We also identified genes encoding hormone-like peptides with a secretion signal and cysteine-rich residues that are conserved among some Solanaceae species, suggesting that peptide hormones may function as signaling molecules during fruit set initiation. This study provides important information about pistil-specific genes, which may play specific roles in regulating pistil development in relation to fruit set.

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“…Using Trp53 -/ -mice, highly susceptible to tumour formation, it was found that inclusion in the diet of transgenic tomatoes which had a high content of anthocyanins (giving them a strong purple colour), by overexpression of two appropriate transcription factors, caused a significant extension of the lifespan of the mice, with respect to controls with their diet supplemented with normal tomatoes (Butelli et al, 2008). Skin extracts of a different anthocyanin-rich, purple tomato variety -in this case, obtained by conventional breedingalso inhibited cell proliferation of two human tumour cell lines in culture (Mazzucato et al, 2013). The effect of lifespan increase is also not specific for resveratrol, as it has been shown as well for other antioxidant polyphenols.…”

Section: Beyond Resveratrol: Flavonoids and Other Phenolic Compoundsmentioning

confidence: 89%

Flavonoids: Antioxidant Compounds for Plant Defence... and for a Healthy Human Diet

Vicente

Boscaiu

2018

Not Bot Horti Agrobo

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Flavonoids are a large group of plant phenolics, including almost 10,000 different compounds with a common chemical structure consisting of two aromatic rings, joined by a three-carbon chain generally forming a heterocyclic ring (C6-C3-C6). Interest on these secondary metabolites has increased exponentially in the last years, for its alleged beneficial effects on human health. It has been reported that flavonoids -and other phenolics -show antibacterial, antiviral, anti-inflammatory, antilipidemic, or antidiabetic activities, and also possess neuroprotective, hepatoprotective or cardioprotective properties. Anthocyanins, particularly, seem to be effective antitumoural compounds, at least in human tumour cell lines and in mouse models. These properties appear to be due to the strong antioxidant character of flavonoids and their capacity to scavenge 'reactive oxygen species' (ROS) which, if in excess, cause oxidative cellular damage. Therefore, flavonoid-rich fruits and vegetables should contribute to a healthy diet. Yet flavonoids are not present in plants for human benefit. They fulfil many disparate biological functions, mostly mediating interactions between plants and the environment: animal attractants for pollination and seed dispersal, signalling molecules in plant-microorganisms interactions, or participating in plant defence against pathogens. They are also involved in the mechanisms of tolerance to practically all types of abiotic stress, including UV radiation, extreme temperatures, ozone exposure, drought or salinity. Since abiotic stresses cause an increase in cellular ROS levels, these latter functions appear to be based on flavonoids' antioxidant activity, similarly to their assumed positive effects for human health if used as dietary components, nutraceuticals or even pharmacological drugs.

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Novel phenotypes related to the breeding of purple-fruited tomatoes and effect of peel extracts on human cancer cell proliferation

Cited by 50 publications

References 33 publications

Phenolic and Anthocyanin Compounds and Antioxidant Activity of Tamarillo (Solanum betaceum Cav.)

Phenolic and Anthocyanin Compounds and Antioxidant Activity of Tamarillo (Solanum betaceum Cav.)

Genome-wide identification of pistil-specific genes expressed during fruit set initiation in tomato (Solanum lycopersicum)

Flavonoids: Antioxidant Compounds for Plant Defence... and for a Healthy Human Diet

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