Novel pH-sensitive charge-reversal cell penetrating peptide conjugated PEG-PLA micelles for docetaxel delivery: In vitro study

Ouahab, Ammar; Cheraga, Nihad; Onoja, Vitus; Shen, Yan; Tu, Jiasheng

doi:10.1016/j.ijpharm.2014.03.009

Cited by 57 publications

(32 citation statements)

References 43 publications

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“…44 Aside from low polymer-drug compatibility and interactions, low efficiency may also be due to the high degree of interdigitation of the butyl chains in the core, which logically reduced the available space for MH and hampered its encapsulation. 29 The addition of TPGS to HA-PBCA led to a slight decrease in the nanoparticles size (134.73±1.85) ( Figure 4A), and increase in negative zeta potential (-31.72±0.17), thereby confirming the stabilizing ability of TPGS. Interestingly, the addition of TPGS resulted also in a significant increase in DL, proportional with the elevation in the TPGS feed ratio.…”

mentioning

confidence: 64%

“…The medium was replaced by 0.25 mg/mL of C6-PNs or C6-MNs diluted in serum-free cell culture medium (C6 content: 500 ng/mL). 29 After 2, 4, and 6 hours of incubation, the culture media were removed, and the cells were rinsed with PBS thrice. Subsequently, the cells were fixed with 4% formaldehyde for 10 minutes and observed under a fluorescence microscope (IX71; Olympus Corp, Tokyo, Japan) 30 and a Leica confocal laser scanning microscope (CLSM) (TCS SP5; Leica, Heidelberg, Germany).…”

Section: In Vitro Cellular Uptake Fluorescence Microscopymentioning

confidence: 99%

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Preparation, in vitro and in vivo evaluation of polymeric nanoparticles based on hyaluronic acid-poly(butyl cyanoacrylate) and D-alpha-tocopheryl polyethylene glycol 1000 succinate for tumor-targeted delivery of morin hydrate

Abbad¹,

Wang²,

Waddad³

et al. 2015

IJN

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Herein, we describe the preparation of a targeted cellular delivery system for morin hydrate (MH), based on a low-molecular-weight hyaluronic acid-poly(butyl cyanoacrylate) (HA-PBCA) block copolymer. In order to enhance the therapeutic effect of MH, D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) was mixed with HA-PBCA during the preparation process. The MH-loaded HA-PBCA “plain” nanoparticle (MH-PNs) and HA-PBCA/TPGS “mixed” nanoparticles (MH-MNs) were concomitantly characterized in terms of loading efficiency, particle size, zeta potential, critical aggregation concentration, and morphology. The obtained MH-PNs and MH-MNs exhibited a spherical morphology with a negative zeta potential and a particle size less than 200 nm, favorable for drug targeting. Remarkably, the addition of TPGS resulted in about 1.6-fold increase in drug-loading. The in vitro cell viability experiment revealed that MH-MNs enhanced the cytotoxicity of MH in A549 cells compared with MH solution and MH-PNs. Furthermore, blank MNs containing TPGS exhibited selective cytotoxic effects against cancer cells without diminishing the viability of normal cells. In addition, the cellular uptake study indicated that MNs resulted in 2.28-fold higher cellular uptake than that of PNs, in A549 cells. The CD44 receptor competitive inhibition and the internalization pathway studies suggested that the internalization mechanism of the nanoparticles was mediated mainly by the CD44 receptors through a clathrin-dependent endocytic pathway. More importantly, MH-MNs exhibited a higher in vivo antitumor potency and induced more tumor cell apoptosis than did MH-PNs, following intravenous administration to S180 tumor-bearing mice. Overall, the results imply that the developed nanoparticles are promising vehicles for the targeted delivery of lipophilic anticancer drugs.

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confidence: 64%

Section: In Vitro Cellular Uptake Fluorescence Microscopymentioning

confidence: 99%

Preparation, in vitro and in vivo evaluation of polymeric nanoparticles based on hyaluronic acid-poly(butyl cyanoacrylate) and D-alpha-tocopheryl polyethylene glycol 1000 succinate for tumor-targeted delivery of morin hydrate

Abbad¹,

Wang²,

Waddad³

et al. 2015

IJN

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“…Furthermore, with the cargo carrying properties of CPPs, they are finding use in the delivery of cancer drugs such as doxorubicin [248,355], taxol [356], paclitaxel [357], docetaxel [358]. Using peptide conjugates for drug delivery into the cell has been a topic of interest and there are many anticancer [359], antiinflammatory [360] and neurodegenerative [361] peptide conjugate drugs that are in clinical use or under investigation.…”

Section: Future Perspectives: Characterizing Membrane Active Peptidesmentioning

confidence: 99%

Membrane Active Peptides and Their Biophysical Characterization

Avcı

Akbulut

Özkırımlı

2018

Preprint

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In the last 20 years, an increasing number of studies have been reported on membrane active peptides, which exert their biological activity by interacting with the cell membrane either to disrupt it and lead to cell lysis or to translocate through it to deliver cargos into the cell and reach their target. These peptides are attractive alternatives to currently used pharmaceuticals. Antimicrobial peptides (AMPs) and peptides designed for drug and gene delivery currently in the drug pipeline suggest that these membrane active peptides will soon constitute a significant percentage of the drug market. Here, we focus on two most prominent classes of membrane active peptides; AMPs and cell-penetrating peptides (CPPs). AMPs are a group of membrane active peptides that disrupt the membrane integrity or inhibit the cellular functions of bacteria, virus and fungi. CPPs are another group of membrane active peptides that mainly function as cargo-carriers even though they may also show antimicrobial activity to some extent. Biophysical techniques to understand how they interact with the membrane have shed light on the peptide-membrane interaction at various levels of detail. Structural investigation of membrane active peptides in the presence of the membrane provides important clues on the effect of the membrane environment on peptide conformations. Advances in live imaging techniques have allowed examination of peptide action at a single cell or single molecule level. In addition to these experimental biophysical techniques, molecular dynamics simulations provided clues on the peptide-lipid interactions and dynamics of the cell entry process at atomic detail. In this review, we summarize the recent advances in experimental and computational investigation of membrane active peptides with particular emphasis on two amphipathic membrane active peptides, the AMP melittin and the CPP pVEC.

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“…Among those efforts, amphiphilic copolymers are widely used in formulation due to their unique self-assembling property in forming core-shell structured NPs (Jeong, Na, Oh, Choi, Song & Lee, 2006;Ouahab, Cheraga, Onoja, Shen & Tu, 2014). NPs (1-500 nm) are good carriers to ferry theranostic agents to their target sites in a precise, safe and efficient manner (Su et al, 2014;Yaw, Wang, Zhou & Yang, 2016).…”

Section: Introductionmentioning

confidence: 99%

Efficient delivery of paclitaxel into ASGPR over-expressed cancer cells using reversibly stabilized multifunctional pullulan nanoparticles

Huang

Wang

Ling

et al. 2017

Carbohydrate Polymers

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Novel pH-sensitive charge-reversal cell penetrating peptide conjugated PEG-PLA micelles for docetaxel delivery: In vitro study

Cited by 57 publications

References 43 publications

Preparation, in vitro and in vivo evaluation of polymeric nanoparticles based on hyaluronic acid-poly(butyl cyanoacrylate) and D-alpha-tocopheryl polyethylene glycol 1000 succinate for tumor-targeted delivery of morin hydrate

Preparation, in vitro and in vivo evaluation of polymeric nanoparticles based on hyaluronic acid-poly(butyl cyanoacrylate) and D-alpha-tocopheryl polyethylene glycol 1000 succinate for tumor-targeted delivery of morin hydrate

Membrane Active Peptides and Their Biophysical Characterization

Efficient delivery of paclitaxel into ASGPR over-expressed cancer cells using reversibly stabilized multifunctional pullulan nanoparticles

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