Novel peptides with anti-proliferation activity from the Porphyra haitanesis hydrolysate

Fan, Xiaodan; Bai, Lu; Mao, Xinliang; Zhang, Xuewu

doi:10.1016/j.procbio.2017.05.018

Cited by 37 publications

(21 citation statements)

References 18 publications

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“…Our results revealed that the <3 kDa UF and >3 kDa UF fractions exhibited similar levels of cytotoxicity against the HeLa cells. Similar levels of cytotoxicity were also observed when <3 kDa UF and >3 kDa UF fractions of a Porphyra haitanesis hydrolysate were tested on the A549 lung cancer and SGC-7901 gastric cancer cell lines (de Lumen, 2005;Fan et al, 2017). Nevertheless, the <3 kDa UF frac-tion of X. testudinaria papain hydrolysate exhibited about seven times stronger cytotoxicity than the 3-10 kDa UF fraction (Quah et al, 2017).…”

Section: Discussionsupporting

confidence: 62%

“…When compared with fractions of larger molecular weight (MW) ranges, the <3 kDa UF fraction prepared from loach papain hydrolysate also exhibited stronger antiproliferative activity against the HepG2, MCF-7, and Caco-2 colon cancer cell lines (You et al, 2011). Furthermore, according to Fan et al (2017), short peptides can be identified easily by MS and are easier to synthesize. Small peptides are also less costly to synthesize (Chai et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…This result is interesting in the light of some other studies in which GF fractions were found to have poorer cytotoxicity than 5-FU. For example, the GF fractions derived from Spirulina (Arthrospira) platensis (Wang and Zhang, 2016) and P. haitanesis (Fan et al, 2017) exhibited weaker cytotoxicity on the MCF-7, HepG2, SGC-7901, A549, and HT-29 cell lines than 5-FU.…”

Section: Discussionmentioning

confidence: 99%

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Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Quah

Ismail

Ooi

et al. 2019

J. Zhejiang Univ. Sci. B

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Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC 50) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC 50 , AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Quah

Ismail

Ooi

et al. 2019

J. Zhejiang Univ. Sci. B

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“…One of the disadvantages of IEX‐based LC separations is that it requires the elimination of ionic components (salts) prior to peptide identification. In some instances only one mode of chromatographic separation is applied (for instance, SEC [92,93], RP [70,90,94,95], or IEX [85]) and the most bioactive fraction is then selected for subsequent peptide identification. However, in the majority of the cases, combinations of LC modes are used in order to maximize the purity of the peptides prior to peptide identification.…”

Section: Separation and Fractionation Of Bapsmentioning

confidence: 99%

“…MALDI is usually coupled with a TOF mass analyzer. A number of examples of the use of MALDI‐TOF for the identification of seaweed BAPs have been reported [81,92,93]. Peptide sequencing can then be achieved by the use of specific software systems that process the data from the MS detector into MS or MS/MS spectra, e.g., PEAKS™ and Mascot™.…”

Section: Identification Of Bapsmentioning

confidence: 99%

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

et al. 2020

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Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed Seaweed (macroalgae) is considered as a sustainable bioresource rich in high-quality nutrients such as protein. Seaweed protein can be used as an alternative to other protein sources. Furthermore, these proteins are natural reservoirs of bioactive peptides (BAPs) associated with various health benefits such as antioxidant, antihypertensive, and antidiabetic activities. However, seaweed-derived BAPs remain underexploited due to challenges that arise during protein extraction from algal biomass. Coupled with this, limited proteomic information exists regarding certain seaweed species. This review highlights the current state of the art of seaweed protein extraction techniques, e.g., liquid, ultrasound, microwave, pulsed electric field, and high hydrostatic pressure assisted extraction. The review also focuses on the enzymatic hydrolysis of seaweed proteins and characterization of the resultant hydrolysates/peptides using electrophoretic and chromatographic techniques. This includes reference to methods employed for separation, fractionation, and purification of seaweed BAPs, as well as the methodologies used for identification, e.g., analysis by mass spectrometry. Furthermore, a bioinformatics or in silico approach to aid discovery of seaweed BAPs is discussed herein. Based on the information available to date, it is suggested that further research is required in this area for the development of seaweed BAPs for nutraceutical applications.

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Marine‐Derived Sources of Nutritional Vitamins

Dashputre¹,

Sable²,

Sawant

et al. 2023

Vitamins as Nutraceuticals

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Novel peptides with anti-proliferation activity from the Porphyra haitanesis hydrolysate

Cited by 37 publications

References 18 publications

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

Marine‐Derived Sources of Nutritional Vitamins

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