Novel Peptide from the Hydrolysate of Hybrid Sturgeon (Acipenseridae) Spinal Cord: Isolation, Identification, and Anti-proliferative Effects in Human Cervix Cancer Cells

Abstract

Anti-proliferative peptides have recently attracted attention for their excellent bioactivity and biocompatibility. In this paper, five novel anti-proliferative peptides were identified from the hydrolysate of hybrid sturgeon spinal cord (HSSC). In addition, the structure−activity relationship of the novel anti-proliferative peptides was explored. In vitro experiments indicated that the peptide "VDSVLDVVRK" presented the highest inhibition of HeLa cell growth in all samples (IC 50 = 2.5 μM). VDSVLDVVRK showed a random coil secondary structure and nanomicelles in the tumor microenvironment. Transmission electron microscopy results confirmed that nanomicelles disassemble as the concentration of VDSVLDVVRK decreases. Furthermore, VDSVLDVVRK could induce HeLa cell apoptosis by increasing the expression of Cyt-c (98.65 ± 1.85%, p < 0.01) and caspase-9 (39.85 ± 1.81%, p < 0.01). In this study, the anti-proliferative mechanism of the HSSC peptide was discussed, which provided a theoretical basis for the research and development of anti-proliferative functional food.

Identification and Verification of Novel Umami Peptides Isolated from Hybrid Sturgeon Meat (Acipenser baerii × Acipenser schrenckii)

“…14 In the past decade, China's sturgeon production has increased dramatically. 15 Raposo et al studies indicated that sturgeon meat as a byproduct of caviar was not effectively utilized. 16 Our previous studies have focused on the exploration of the flavor of steamed sturgeon meat, and these results showed that umami and saltiness were the characteristic flavors of steamed sturgeon meat.…”

“…Liu et al showed that seven novel umami peptides (HLQLAIR, DPLRGGYY, LLLPGELAK, AGLQFPVGR, GYSFTTTAER, AGFAGDDAPR, and DAGVIAGLNVLR) were isolated from Takifugu rubripes, which possessed excellent umami characteristics . In the past decade, China’s sturgeon production has increased dramatically . Raposo et al studies indicated that sturgeon meat as a byproduct of caviar was not effectively utilized .…”

Tuna trimmings (Thunnas albacares) hydrolysate alleviates immune stress and intestinal mucosal injury during chemotherapy on mice and identification of potentially active peptides

“…It was reported that the trypsin hydrolyzed fraction of MW < 1 kDa, produced from the spinal cord of hybrid sturgeon ( Acipenseridae ), showed 34.84 ± 2.86% antiproliferative activity against HeLa cells at a concentration of 200 μg/mL. Additionally, this peptides fraction displayed a proliferative activity of 72.70 ± 2.46% on L929 cells, indicating no significant cytotoxicity ( Han et al, 2022 ).…”