Novel pectin isolated from Spirulina maxima enhances the disease resistance and immune responses in zebrafish against Edwardsiella piscicida and Aeromonas hydrophila

Edirisinghe, Shan Lakmal; Dananjaya, S.H.S.; Nikapitiya, Chamilani; Liyanage, T.D.; Lee, Kyoung-Ah; Oh, Chulhong; Kang, Do‐Hyung; Zoysa, Mahanama De

doi:10.1016/j.fsi.2019.09.054

Cited by 31 publications

(11 citation statements)

References 30 publications

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“…Furthermore, Spirulina-derived C-phycocyanin, increased the secretion of inflammatory cytokines TNF-α, IL-1β, and IL-6 in murine macrophages [10]. In our previous study, we isolated the pectin from S. maxima (SmP) and described the immune modulation in zebrafish [11]. Moreover, preliminary experimental results indicate the potential prebiotic and wound healing effects of SmP in zebrafish model (unpublished data).…”

Section: Introductionmentioning

confidence: 91%

Marine Microalgae, Spirulina maxima-Derived Modified Pectin and Modified Pectin Nanoparticles Modulate the Gut Microbiota and Trigger Immune Responses in Mice

et al. 2020

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This study evaluated the modulation of gut microbiota, immune responses, and gut morphometry in C57BL/6 mice, upon oral administration of S. maxima-derived modified pectin (SmP, 7.5 mg/mL) and pectin nanoparticles (SmPNPs; 7.5 mg/mL). Metagenomics analysis was conducted using fecal samples, and mice duodenum and jejunum were used for analyzing the immune response and gut morphometry, respectively. The results of metagenomics analysis revealed that the abundance of Bacteroidetes in the gut increased in response to both modified SmP and SmPNPs (75%) as compared with that in the control group (66%), while that of Firmicutes decreased in (20%) as compared with that in the control group (30%). The mRNA levels of mucin, antimicrobial peptide, and antiviral and gut permeability-related genes in the duodenum were significantly (p < 0.05) upregulated (> 2-fold) upon modified SmP and SmPNPs feeding. Protein level of intestinal alkaline phosphatase was increased (1.9-fold) in the duodenum of modified SmPNPs feeding, evidenced by significantly increased goblet cell density (0.5 ± 0.03 cells/1000 µm2) and villi height (352 ± 10 µm). Our results suggest that both modified SmP and SmPNPs have the potential to modulate gut microbial community, enhance the expression of immune related genes, and improve gut morphology.

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Section: Introductionmentioning

confidence: 91%

Marine Microalgae, Spirulina maxima-Derived Modified Pectin and Modified Pectin Nanoparticles Modulate the Gut Microbiota and Trigger Immune Responses in Mice

et al. 2020

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“…Zebrafish mating, embryo collection, and maintenance were conducted according to the method described by Edirisighe et al [38]. In brief, 60 h post-fertilized larvae (n = 10) were exposed to different concentrations (0, 10, 25, 50, and 100 µg/mL) of either Octominin-CNPs, Octominin, or CNPs for 96 h. Larval mortality was observed at 12 h intervals of post-treatment, and growth abnormalities and behavioral changes were examined for 96 h. Four larvae from each treatment group (at 96 hpt) were subjected to measure the ROS levels by staining with H 2 DCFDA (Sigma-Aldrich, St. Louis, MO, USA) according to the method described by Edirisighe et al [38]. Briefly, larvae were exposed to H 2 DCFDA (5 µg/mL) for 30 min, and the excess stain was removed by washing thrice with embryonic media.…”

Section: Determination Of In Vivo Toxicity Of Octominin-cnpsmentioning

confidence: 99%

Antimicrobial Peptide Octominin-Encapsulated Chitosan Nanoparticles Enhanced Antifungal and Antibacterial Activities

Jayathilaka

Nikapitiya

Zoysa

et al. 2022

IJMS

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Antimicrobial peptides (AMPs) have become a key solution for controlling multi-drug-resistant (MDR) pathogens, and the nanoencapsulation of AMPs has been used as a strategy to overcome challenges, such as poor stability, adverse interactions, and toxicity. In previous studies, we have shown the potent antimicrobial activity of Octominin against Candida albicans and Acinetobacter baumannii. This study is focused on the nanoencapsulation of Octominin with chitosan (CS) and carboxymethyl chitosan (CMC) as a drug delivery system using the ionotropic gelation technique. Octominin-encapsulated CS nanoparticles (Octominin-CNPs) had an average diameter and zeta potential of 372.80 ± 2.31 nm and +51.23 ± 0.38 mV, respectively, while encapsulation efficiency and loading capacity were 96.49 and 40.20%, respectively. Furthermore, Octominin-CNPs showed an initial rapid and later sustained biphasic release profile, and up to 88.26 ± 3.26% of the total Octominin release until 96 h. Transmission electron microscopy data showed the irregular shape of the Octominin-CNPs with aggregations. In vitro and in vivo toxicity of Octominin-CNPs was significantly lower than the Octominin at higher concentrations. The antifungal and antibacterial activities of Octominin-CNPs were slightly higher than those of Octominin in both the time-kill kinetic and microbial viability assays against C. albicans and A. baumannii, respectively. Mode of action assessments of Octominin-CNPs revealed that morphological alterations, cell membrane permeability alterations, and reactive oxygen species generation were slightly higher than those of Octominin at the tested concentrations against both C. albicans and A. baumannii. In antibiofilm activity assays, Octominin-CNPs showed slightly higher biofilm inhibition and biofilm eradication activities compared to that of Octominin. In conclusion, Octominin was successfully encapsulated into CS, and Octominin-CNPs showed lower toxicity and greater antimicrobial activity against C. albicans and A. baumannii compared to Octominin.

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“…Several herbal compounds have been shown to modulate immune responses and treat infections. Plants from the Lamiaceae family (mints) have been studied in several recent experiments [ 60 , 66 , 67 , 68 , 73 , 74 ]. One study by Abdellatif et al reported a significant effect of combined sage ( Salvia officinalis ) and Spirulina platensis ( Arthrospira platensis ) on levels of immune markers, including lysozyme, nitric oxide, and immunoglobulin M (IgM), as well as increased resistance against Pseudomonas aeruginosa in Nile tilapia [ 68 ].…”

Section: The Effect Of Herbal Extracts and Plant Components On Immune-related Genes In Fish Speciesmentioning

confidence: 99%

“…In addition to the abovementioned components, blue-green algae or spirulina can be a potential feed supplement for the health and welfare of marine species. Pectin, the main ingredient of the cell wall in spirulina, showed an immunomodulatory effect in zebrafish via up-regulation of pro-inflammatory cytokines, chemokines, lysozyme, and mucin as a medication against E. piscicida and A. hydrophila [ 67 ]. The combination of spirulina with selenium nanoparticles can trigger immune responses via activation of liver SOD and TNF-α gene expressions and suppression of HSP70 in Nile tilapia [ 58 ].…”

Section: The Effect Of Herbal Extracts and Plant Components On Immune-related Genes In Fish Speciesmentioning

confidence: 99%

The Gene Regulatory Roles of Herbal Extracts on the Growth, Immune System, and Reproduction of Fish

Ahmadifar

Fallah

Yousefi

et al. 2021

Animals

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The crucial need for safe and healthy aquatic animals obligates researchers in aquaculture to investigate alternative and beneficial additives. Medicinal herbals and their extracts are compromised with diverse effects on the performances of aquatic animals. These compounds can affect growth performance and stimulate the immune system when used in fish diet. In addition, the use of medicinal herbs and their extracts can reduce oxidative stress induced by several stressors during fish culture. Correspondingly, aquatic animals could gain increased resistance against infectious pathogens and environmental stressors. Nevertheless, the exact mode of action where these additives can affect aquatic animals’ performances is still not well documented. Understanding the mechanistic role of herbal supplements and their derivatives is a vital tool to develop further the strategies and application of these additives for feasible and sustainable aquaculture. Gene-related studies have clarified the detailed information on the herbal supplements’ mode of action when administered orally in aquafeed. Several review articles have presented the potential roles of medicinal herbs on the performances of aquatic animals. However, this review article discusses the outputs of studies conducted on aquatic animals fed dietary, medicinal herbs, focusing on the gene expression related to growth and immune performances. Furthermore, a particular focus is directed to the expected influence of herbal supplements on the reproduction of aquatic animals.

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Novel pectin isolated from Spirulina maxima enhances the disease resistance and immune responses in zebrafish against Edwardsiella piscicida and Aeromonas hydrophila

Cited by 31 publications

References 30 publications

Marine Microalgae, Spirulina maxima-Derived Modified Pectin and Modified Pectin Nanoparticles Modulate the Gut Microbiota and Trigger Immune Responses in Mice

Marine Microalgae, Spirulina maxima-Derived Modified Pectin and Modified Pectin Nanoparticles Modulate the Gut Microbiota and Trigger Immune Responses in Mice

Antimicrobial Peptide Octominin-Encapsulated Chitosan Nanoparticles Enhanced Antifungal and Antibacterial Activities

The Gene Regulatory Roles of Herbal Extracts on the Growth, Immune System, and Reproduction of Fish

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