Novel paclitaxel nanoparticles: Development,  in vitro  anti-tumor activity in BT-549 cells and i n vivo  evaluation

Shavi, Gopal Venkatesh; Kumar, Avinash; Karthik, A.; Naseer, Md. Arif; Aravind, G.; Praful, B.D.; Reddy, M. Sreenivasa; Udupa, N.

doi:10.1016/j.jconrel.2010.07.091

Cited by 3 publications

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“…Particle size and size distribution are important physicochemical properties that determine both uptake and biological fate of the particulate systems [31]. In the results, important effects of D/P and W/O ratios on size of NPs were confirmed.…”

Section: Discussionmentioning

confidence: 76%

Preparation, characterization and optimization of sildenafil citrate loaded PLGA nanoparticles by statistical factorial design

et al. 2013

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Background and the aim of the studyThe objective of the present study was to formulate and optimize nanoparticles (NPs) of sildenafil-loaded poly (lactic-co-glycolic acid) (PLGA) by double emulsion solvent evaporation (DESE) method. The relationship between design factors and experimental data was evaluated using response surface methodology.MethodA Box-Behnken design was made considering the mass ratio of drug to polymer (D/P), the volumetric proportion of the water to oil phase (W/O) and the concentration of polyvinyl alcohol (PVA) as the independent agents. PLGA-NPs were successfully prepared and the size (nm), entrapment efficiency (EE), drug loading (DL) and cumulative release of drug from NPs post 1 and 8 hrs were assessed as the responses.ResultsThe NPs were prepared in a spherical shape and the sizes range of 240 to 316 nm. The polydispersity index of size was lower than 0.5 and the EE (%) and DL (%) varied between 14-62% and 2-6%, respectively. The optimized formulation with a desirability factor of 0.9 was selected and characterized. This formulation demonstrated the particle size of 270 nm, EE of 55%, DL of 3.9% and cumulative drug release of 79% after 12 hrs. In vitro release studies showed a burst release at the initial stage followed by a sustained release of sildenafil from NPs up to 12 hrs. The release kinetic of the optimized formulation was fitted to Higuchi model.ConclusionsSildenafil citrate NPs with small particle size, lipophilic feature, high entrapment efficiency and good loading capacity is produced by this method. Characterization of optimum formulation, provided by an evaluation of experimental data, showed no significant difference between calculated and measured data.

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Section: Discussionmentioning

confidence: 76%