Novel natural spider silk embedded electrospun nanofiber mats for wound healing

Öksüz, Keri̇m Emre; Özkaya, Neşe Kurt; İnan, Zeynep Deniz Şahin; Özer, Ali

doi:10.1016/j.mtcomm.2020.101942

Cited by 17 publications

(9 citation statements)

References 29 publications

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“…For example, alginate NFs were combined with natural spider silk and assessed as wound dressing materials by comparing their physical-chemical and biological properties with commercially available products. [246] In this sense, these composite NFs accelerated the rate of wound healing by improving the collagen formation rate and proliferative cell activity, as well as by decreasing the inflammatory cell amount as reported in Figure 12e. The unique complexation capability of alginate and chitosan has also been exploited to fabricate in situ crosslinked stable electrospun NFs.…”

Section: Tissue Engineering and Wound Healingmentioning

confidence: 76%

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An Up‐to‐Date Review on Alginate Nanoparticles and Nanofibers for Biomedical and Pharmaceutical Applications

Dodero

Alberti

Gaggero

et al. 2021

Adv Materials Inter

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along with other polysaccharides (e.g., celluloses, chitosan, etc.), is indeed considered an extremely promising material in the viewpoint of circular economy and in substituting petroleum-derived polymers. [3] Among others, one of the reasons for alginate success is its unique capability to bind different cations leading to stable and tailor-made hydrogels. [4] Owing to its biocompatibility, biodegradability, and nontoxicity, in the past decades alginate has been vastly explored for drug and gene delivery, tissue engineering, and wound healing applications. [5] In this sense, the recent nanotechnological advances have allowed the fabrication and exploitation of alginate-based nanostructured materials with completely new capabilities. Thereby, it is not surprising that nowadays a broad variety of alginate-based nanomaterials with various shapes, sizes, and compositions are prepared via different approaches, including controlled gelation, electrospinning and electrospraying, self-assembly, phase-separation, and micro fluidics., All these nanostructures hold the potential to interact with living organisms much more efficiently with respect to bulk systems, hence leading to specific functionalities at the same time avoiding the occurrence of toxicity issues. [6][7][8] Despite the use of alginate-based nanomaterials has been reviewed in the past, the focus has been commonly pointed to their generic properties and applications. Conversely, this review attempts to provide a more specific and comprehensive summary limited to the most recent advances in the fabrication and use of two of the most common and promising alginatebased nanomaterials, namely nanoparticles (NPs) and electrospun nanofibers (NFs), with a focus on biomedical and pharmaceutical applications.

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Section: Tissue Engineering and Wound Healingmentioning

confidence: 76%

mentioning

confidence: 99%

An Up‐to‐Date Review on Alginate Nanoparticles and Nanofibers for Biomedical and Pharmaceutical Applications

Dodero

Alberti

Gaggero

et al. 2021

Adv Materials Inter

View full text Add to dashboard Cite

show abstract

“…In a recent investigation, natural spider silk was embedded into SA/PVA nanofibrous scaffolds. In vivo tests on rabbit models and in vitro biocompatibility with cellular behavior assays on murine fibroblast cells (L929) indicated that the nanofibrous mat loaded with the natural spider silk induced rapid healing of skin scars by decreasing the numbers of inflammatory cells and improving the collagen formation rate and proliferative cell activity ( Figure 3 A) [ 56 ]. In a recent work, ZnO nanoparticles, acting as an antimicrobial agent, were encapsulated in alginate/PVA electrospun nanofibers, providing remarkable antimicrobial activity against E. coli , S. aureus , and Candida albicans [ 57 ].…”

Section: Seaweed-derived Biopolymersmentioning

confidence: 99%

“…SEM images of collagen deposition under the wound healing site at 7 days post-treatment with ( e ) PVA/SA and ( f ) PVA/SA/spider silk nanofibers. Reprinted/adapted with permission from [ 56 ], Copyright 2021, Elsevier. ( B ) ( a ) SEM image of gentamicin-loaded alginate/chitosan (CS/Alg/3-Gn) nanofibers.…”

Section: Seaweed-derived Biopolymersmentioning

confidence: 99%

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

et al. 2022

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Marine biopolymers, abundantly present in seaweeds and marine animals, feature diverse structures and functionalities, and possess a wide range of beneficial biological activities. Characterized by high biocompatibility and biodegradability, as well as unique physicochemical properties, marine biopolymers are attracting a constantly increasing interest for the development of advanced systems for applications in the biomedical field. The development of electrospinning offers an innovative technological platform for the production of nonwoven nanofibrous scaffolds with increased surface area, high encapsulation efficacy, intrinsic interconnectivity, and structural analogy to the natural extracellular matrix. Marine biopolymer-based electrospun nanofibrous scaffolds with multifunctional characteristics and tunable mechanical properties now attract significant attention for biomedical applications, such as tissue engineering, drug delivery, and wound healing. The present review, covering the literature up to the end of 2021, highlights the advancements in the development of marine biopolymer-based electrospun nanofibers for their utilization as cell proliferation scaffolds, bioadhesives, release modifiers, and wound dressings.

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“…They have been used as sutures for wound healing for centuries ( Altman et al, 2003 ). Because of these properties, spider silks are regarded as a promising material for medical applications such as selective microbial-resistant coatings ( Kumari et al, 2020 ), organic and degradable biosensors for biomonitoring of analytes in the body ( Xu et al, 2019 ), wound healing ( Öksüz et al, 2021 ), creating lenses useful for biological imaging ( Tian et al, 2020 ), tissue engineering ( Salehi et al, 2020 ) such as artificial blood vessels ( Dastagir et al, 2020 ), nerve regeneration ( Kornfeld et al, 2021 ; Millesi et al, 2021 ) and scaffolds creation ( Gellynck et al, 2008 ). In addition, these silks have potential for use as smart materials.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Spider Silk: Promises and Bottlenecks

Ramezaniaghdam

Nahdi

Reski

2022

Front. Bioeng. Biotechnol.

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Spider silk threads have exceptional mechanical properties such as toughness, elasticity and low density, which reach maximum values compared to other fibre materials. They are superior even compared to Kevlar and steel. These extraordinary properties stem from long length and specific protein structures. Spider silk proteins can consist of more than 20,000 amino acids. Polypeptide stretches account for more than 90% of the whole protein, and these domains can be repeated more than a hundred times. Each repeat unit has a specific function resulting in the final properties of the silk. These properties make them attractive for innovative material development for medical or technical products as well as cosmetics. However, with livestock breeding of spiders it is not possible to reach high volumes of silk due to the cannibalistic behaviour of these animals. In order to obtain spider silk proteins (spidroins) on a large scale, recombinant production is attempted in various expression systems such as plants, bacteria, yeasts, insects, silkworms, mammalian cells and animals. For viable large-scale production, cost-effective and efficient production systems are needed. This review describes the different types of spider silk, their proteins and structures and discusses the production of these difficult-to-express proteins in different host organisms with an emphasis on plant systems.

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Novel natural spider silk embedded electrospun nanofiber mats for wound healing

Cited by 17 publications

References 29 publications

An Up‐to‐Date Review on Alginate Nanoparticles and Nanofibers for Biomedical and Pharmaceutical Applications

An Up‐to‐Date Review on Alginate Nanoparticles and Nanofibers for Biomedical and Pharmaceutical Applications

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

Recombinant Spider Silk: Promises and Bottlenecks

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