Novel nanoparticle/enzyme biosilicified nanohybrids for advanced heterogeneously catalyzed protocols

García, Carolina S.; Itabaiana, Ivaldo; Souza, Rodrigo O. M. A. de; Luque, Rafael

doi:10.1039/c4cy01313a

Cited by 15 publications

(37 citation statements)

References 14 publications

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“…These were found to be highly pH ands olventd ependent.T he use of DMSO led to low yields of material (0.88 ga t6 0% v/v)t hat showeda n almostt wo-fold higher activity as compared with that obtained from~40 %s olventc onditions (Table 1). ACN, employed previously as as olvent in lipase bio-silicification studies, [20,24] provided similary ields and immobilised laccase activity as DMSO or MeOH under the same conditions (60 %f inal concentration; Table 1). ACN was henceforth selected as the solvent used in the bio-silicification step in line with its more environmentally friendly nature than that of DMSO or MeOH.O ther greener solvents (i.e.,w ater,e thanol, ethyl acetate) investigated in the bio-silicification process provided almostn egligible activities and low yields of final materials (data not shown).…”

Section: Selection Of Optimum Solvent and Ph For The Immobilisation Ymentioning

confidence: 99%

“…The obtained valuesf or these materials are in good agreement with highly active bio-silicified materials reported previously. [20,24] Selection of the appropriateenzyme for immobilisation/ encapsulation…”

Section: Selection Of Optimum Solvent and Ph For The Immobilisation Ymentioning

confidence: 99%

“…The differences observed suggest am ore drastic effect of the silicification process on different laccases than that shown in previouswork with lipases. [20,24]…”

Section: Selection Of Optimum Solvent and Ph For The Immobilisation Ymentioning

confidence: 99%

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Encapsulated Laccases for the Room‐Temperature Oxidation of Aromatics: Towards Synthetic Low‐Molecular‐Weight Lignins

Pistone

Ottolina

et al. 2016

ChemSusChem

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A new approach for the encapsulation of laccases with enhanced activity and stability by biomimetic silica mineralisation is reported. A range of lignin model compounds, which includes syringol, syringyl acid, 4-vinylphenol, gallic acid, vanillic acid and guaiacol, was oxidised to lignin-type polymers by the silica-immobilised laccase systems at room temperature. The oxidation rate of the immobilised systems was lower than that of the free enzyme counterparts, but interesting products were observed with the new bio-catalytic materials, which showed reusability and good stability.

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Section: Selection Of Optimum Solvent and Ph For The Immobilisation Ymentioning

confidence: 99%

Section: Selection Of Optimum Solvent and Ph For The Immobilisation Ymentioning

confidence: 99%

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Encapsulated Laccases for the Room‐Temperature Oxidation of Aromatics: Towards Synthetic Low‐Molecular‐Weight Lignins

Pistone

Ottolina

et al. 2016

ChemSusChem

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“…3,6,9,18 Hence, in current study, the MCNT immobilized lipase was subjected for acetate synthesis at 5 temperature ranging from 25-55 o C (Table 6, entries [28][29][30][31][32][33][34]. The increase of temperature from 25-50 o C led to increase in % conversion of the desired product and thus 50 o C was observed to be optimum temperature for immobilized lipase, since 55 o C displayed similar % conversion at given reaction condition.…”

Section: 9mentioning

confidence: 99%

Synthesis of lipase nano-bio-conjugates as an efficient biocatalyst: characterization and activity–stability studies with potential biocatalytic applications

2015

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5In present study, we have synthesized lipase-nano-bio-conjugates via immobilization of various lipases on multiwall carbon nano-tube (MCNT), in order to construct an efficient and recyclable biocatalytic system. In screening study lipase Pseudomonas fluorescens (PFL) acted as an efficient biocatalyst (lipase-nano-bio-conjugates) which showed higher retention of lipase activity and protein loading. Consequently immobilization support:lipase (MCNT:PFL) composition was screened in which MCNT:PFL (2:1) was worked out as robust biocatalyst composition which showed higher activity retention and protein loading. This nano-bio-conjugate was then 10 characterized in details with physical and biochemical techniques using SEM, TEM, FTIR, Km, Vmax, catalytic efficiency and (%) water content analysis. This developed biocatalyst was further used for practical biocatalytic applications such as O-acylation reactions. Various reaction parameters were optimized in details like reactant molar ratio (2:3.5), solvent, MCNT:PFL biocatalyst amount (36 mg), temperature (50 o C) etc. The developed biocatalytic protocol was then extended to synthesize several (twenty-two) industrially important acylated moieties with an excellent yield, these products are well characterized by 1 HNMR, 13 CNMR and GCMS analysis. Moreover in 15 present study, we have reviewed potential industrial applications of various synthesized compounds. Also, we have studied thermodynamic aspect which demonstrated more feasibility of use of immobilized MCNT:PFL lipase over free lipase. Interestingly, immobilized MCNT:PFL lipase showed 2.3 folds higher catalytic activity than free PFL. Besides this, biocatalyst was efficiently recycled upto five cycles. Thus the present protocol demonstrated, (i) synthesis of nano-bio-conjugates as a bio-catalyst, (ii) detail physical-biochemical characterization of nano-bio-conjugates, (iii) optimization of biocatalytic protocol (iv) practical biocatalytic 20 applications along with mechanistic study (v) thermodynamic feasibility study and (vi) recyclability study. 65 conjugates as compared to bulk solid materials. This is because of key properties such as higher surface area, low mass transfer resistance, effective enzyme loading, nano-scale dispersion and ease of surface functionalization/ modification. [10][11][12][13][14][15][16][17][18][19] Thus, discovery of nano-scale materials proved countless 70 applications and scope based on their extraordinary properties

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“…The chitosan nanoparticle is therefore expected to be suitable for the removal of Ni 2+ , to prevent secondary contamination, and to yield a novel and stable complex particle containing high Ni 2+ concentrations that could be suitable for a range of applications. 23,24 A variety of solids have been investigated for use as the support, including silicon particles, 25 magnetic particles, 26 and porous ceramic monoliths. 20 To date, Ni-based carriers have been widely used in protein purification and enzyme immobilization.…”

Section: Introductionmentioning

confidence: 99%

Adsorptive removal of Ni(ii) ions from aqueous solution and the synthesis of a Ni-doped ceramic: an efficient enzyme carrier exhibiting enhanced activity of immobilized lipase

Huang

et al. 2016

RSC Adv.

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We herein report a novel strategy for the removal of heavy metals and the subsequent preparation of a metal ceramic for immobilizing enzymes. To demonstrate this concept, Ni 2+ ions were removed from an aqueous solution via entrapment by chitosan nanoparticles, and the resulting Ni(II)-containing precipitate was mixed with the ceramic matrix to give the Nidoped ceramic (Ni-CP), which was subsequently applied in lipase immobilization. Under optimized conditions, Ni 2+ removal reached 99.4%, and the Ni-CP showed significant chelation towards lipase following immobilization. In addition, a lipase activity yield of 164% was obtained under the optimal conditions. Furthermore, the thermal and storage stabilities of Ni-CP-lipase exhibited a wider applied range, and the Ni-CP reusability was maintained at 97.5% following 20 cycles, suggesting high stability and excellent recyclability. Hence, the entrapped Ni 2+ exhibited improved stability, thus reducing leakage into the environment. Furthermore, the chelation between Ni 2+ and lipase improved enzyme activity and stability, and thus, may be suitable for application in large-scale production. It is therefore expected that this novel approach for enzyme immobilization has potential to serve as an important technique in the field of biocatalysis. ‡ TOCWe report the successful removal of Ni 2+ from aqueous solution via entrapment by chitosan nanoparticles, followed by calcination with a ceramic matrix to construct a novel carrier for lipase immobilization with enhanced activity.

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Novel nanoparticle/enzyme biosilicified nanohybrids for advanced heterogeneously catalyzed protocols

Cited by 15 publications

References 14 publications

Encapsulated Laccases for the Room‐Temperature Oxidation of Aromatics: Towards Synthetic Low‐Molecular‐Weight Lignins

Encapsulated Laccases for the Room‐Temperature Oxidation of Aromatics: Towards Synthetic Low‐Molecular‐Weight Lignins

Synthesis of lipase nano-bio-conjugates as an efficient biocatalyst: characterization and activity–stability studies with potential biocatalytic applications

Adsorptive removal of Ni(ii) ions from aqueous solution and the synthesis of a Ni-doped ceramic: an efficient enzyme carrier exhibiting enhanced activity of immobilized lipase

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