Encapsulated Laccases for the Room‐Temperature Oxidation of Aromatics: Towards Synthetic Low‐Molecular‐Weight Lignins

Pistone, L.; Ottolina, Gianluca; De, Sudipta; Romero, Antonio A.; Martins, Lı́gia O.; Luque, Rafael

doi:10.1002/cssc.201501427

Cited by 15 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As it can be observed in Figure 3, the patterns exhibit two maxima peaks, assigned to (2 1 1) and 2 2 0reflections. In addition, the ratio value d220/d2110 approximated to 0.87 is according with a cubic structure of MCM-48 [8,[20][21][22]. This indicates a certain structural ordering of the biosilicified enzymes.…”

Section: Biocatalysts Characterizationmentioning

confidence: 99%

“…Based on previous reports [20][21][22]25], the proposed one-pot encapsulation technique was evaluated regarding enzyme content to obtain an optimum transesterification activity. [13,16,26].…”

Section: Synthesis and Evaluation Of Biosilicified Pseudomonas Fluorementioning

confidence: 99%

“…By this methodology, recently reported by Luque et al, enzymes are immobilized on the support during the synthesis process. Thus a silicic acid organic precursor is mineralized over the enzyme and this process, denominated biosilicification, can provides an exceptional stability to the biocatalyst under drastic conditions [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Ferrero

Faba

Eimer

2020

Preprint

View full text Add to dashboard Cite

Background: Nowadays, as an alternative to the production of fuels and chemicals from the fossil platform, renewable feedstocks are widely investigated. For biomass conversion, a new generation of catalysts with specific characteristics such as high activity and selectivity, easy recovery and reusability is necessary. The design of highly efficient and stable heterogeneous catalysts represents a challenge in this field, mainly to overcome current energy and environmental issues. The combination of enzymatic and heterogeneous inorganic catalysis generates an unprecedented platform that combines the advantages of both. Among the techniques for producing solid catalysts, enzymatic mineralization with an organic silicic precursor to obtain hybrid biocatalysts (biosilicification) is highlighted. This technique can provide exceptional stability to the biocatalyst in drastic conditions of use.Results: Then, under these criteria, this work presents the one-step synthesis of a solid enzymatic catalyst, denominated Low Ordered Biosilicified Enzyme (LOBE) due to their structural properties. Pseudomonas Fluorescens lipase forms aggregates that are contained in the heart of a silicon-covered micelle, providing active sites with the ability to process different raw materials (commercial sunflower and soybean oil, Jatropha excisa oil, waste frying oil, residual soybeans, and pork fat) to produce first and second generation biodiesel. Obtaining yields between 81 and 93% by weight depending on the used raw material.Conclusions: Therefore, refined, non-edible and residual oils (with high water and free fatty acid contents) can be transformed into biodiesel through LOBE catalysts with commercial ethanol as co-substrate.

show abstract

Section: Biocatalysts Characterizationmentioning

confidence: 99%

Section: Synthesis and Evaluation Of Biosilicified Pseudomonas Fluorementioning

confidence: 99%

See 1 more Smart Citation

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Ferrero

Faba

Eimer

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, Pistone et al reported that they succeeded in immobilizing different laccases by silica mineralization in one-pot process, modifying the immobilization conditions (pH, organic solvent, enzyme content, etc.) [32]. Although the oxidation rates of the biocatalysts were lower than those of the free enzymes, the biosilici ed enzymes showed to be highly stable and reusable [32].…”

Section: Introductionmentioning

confidence: 97%

“…Co-precipitation is one of the commonly used methods to immobilize enzymes, in which proteins are immobilized on a support during its synthesis: a silicon organic precursor (tetraethyl orthosilicate, TEOS) is added to a mixture of dodecylamine (surfactant), acetonitrile (organic solvent) and a buffer solution [31]. This process of enzymatic mineralization is denominated biosilici cation and provides an exceptional biocatalyst stability under drastic conditions [31][32][33]. According to Cebrián-García et al, the conversion of valeric acid and ethanol to ethyl valerate is maintained over 93% e ciency in the presence of biosilici ed Candida antarctica lipase B (Cal-B) [34].…”

Section: Introductionmentioning

confidence: 99%

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Ferrero

Faba

Eimer

2020

Preprint

View full text Add to dashboard Cite

Background: Cumulative reported evidence has indicated that renewable feedstocks are a promising alternative source to fossil platforms for the production of fuels and chemicals. In that regard, the development of new, highly active, selective, and easy to recover and reuse catalysts for biomass conversions is urgently needed. The combination of enzymatic and inorganic heterogeneous catalysis generates an unprecedented platform that combines the advantages of both, the catalytic efficiency and selectivity of enzymes with the ordered structure, high porosity, mechanical, thermal and chemical resistance of mesoporous materials to obtain enzymatic heterogeneous catalysts. Enzymatic mineralization with an organic silicon precursor (biosilicification) is a promising and emerging approach for the generation of solid hybrid biocatalysts with exceptional stability under severe use conditions. Herein, we assessed the putative advantages of the biosilicification technology for developing an improved efficient and stable biocatalyst for sustainable biofuel production. Results: A series of solid enzymatic catalysts denominated LOBE (Low Ordered Biosilicified Enzyme) were synthesized from Pseudomonas fluorescens lipase and tetraethyl orthosilicate. The microscopic structure and physicochemical properties characterization revealed that the enzyme formed aggregates that were contained in the heart of silicon-covered micelles, providing active sites with the ability to process different raw materials (commercial sunflower and soybean oils, Jatropha excisa oil, waste frying oil, acid oil from soybean soapstock, and pork fat) to produce first and second generation biodiesel. Ester content ranged from 81 to 93% wt depending on the raw material used for biodiesel synthesis. Conclusions: A heterogeneous enzymatic biocatalyst, LOBE4, for efficient biodiesel production was successfully developed in a single step synthesis reaction using biosilicification technology. LOBE4 showed to be highly efficient in converting refined, non-edible and residual oils (with high water and free fatty acid contents) and ethanol into biodiesel. Thus, LOBE4 emerges as a promising tool to produce second-generation biofuels, with significant implications for establishing a circular economy and reducing the carbon footprint.

show abstract

Laccase‐Catalyzed Synthesis of Low‐Molecular‐Weight Lignin‐Like Oligomers and their Application as UV‐Blocking Materials

Lim

Sana²,

Krishnan

et al. 2018

Chemistry An Asian Journal

View full text Add to dashboard Cite

The laccase-catalyzed oxidative polymerization of monomeric and dimeric lignin model compounds was carried out with oxygen as the oxidant in aqueous medium. The oligomers were characterized by using gel permeation chromatography (GPC) and matrix-assisted laser desorption ionization time-of-flight mass spectroscopy (MALDI-TOF MS) analysis. Oxidative polymerization led to the formation of oligomeric species with a number-average molecular weight (M ) that ranged from 700 to 2300 Da with a low polydispersity index. Spectroscopic analysis provided insight into the possible modes of linkages present in the oligomers, and the oligomerization is likely to proceed through the formation of C-C linkages between phenolic aromatic rings. The oligomers were found to show good UV light absorption characteristics with high molar extinction coefficient (5000-38 000 m cm ) in the UV spectral region. The oligomers were blended independently with polyvinyl chloride (PVC) by using solution blending to evaluate the compatibility and UV protection ability of the oligomers. The UV/Vis transmittance spectra of the oligomer-embedded PVC films indicated that these lignin-like oligomers possessed a notable ability to block UV light. In particular, oligomers obtained from vanillyl alcohol and the dimeric lignin model were found to show good photostability in accelerated UV weathering experiments. The UV-blocking characteristics and photostability were finally compared with the commercial low-molecular-weight UV stabilizer 2,4-dihydroxybenzophenone.

show abstract

Encapsulated Laccases for the Room‐Temperature Oxidation of Aromatics: Towards Synthetic Low‐Molecular‐Weight Lignins

Cited by 15 publications

References 37 publications

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Biodiesel production from alternative raw materials using a heterogeneous Low Ordered Biosilicified Enzyme as biocatalyst

Laccase‐Catalyzed Synthesis of Low‐Molecular‐Weight Lignin‐Like Oligomers and their Application as UV‐Blocking Materials

Contact Info

Product

Resources

About