Novel multiplex fluorescent immunoassays based on quantum dot nanolabels for mycotoxins determination

Beloglazova, Natalia V.; Speranskaya, E. S.; Wu, Aibo; Wang, Z.; Sanders, Melanie; Goftman, Valentina V.; Zhang, D.; Goryacheva, Irina Yu.; Saeger, Sarah De

doi:10.1016/j.bios.2014.06.021

Cited by 119 publications

(56 citation statements)

References 43 publications

(44 reference statements)

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“…Immunoassay and aptamer principles have been developed to detect multiple mycotoxins. Wu et al established multiplexed fluorescence resonance energy transfer aptasensor between upconversion nanoparticles and graphene oxide for the simultaneous determination of OTA and FB1 with the LOD of 0.02 ng/mL for OTA and 0.1 ng/mL for FB 1 [101]. Chen et al applied antigen-modified magnetic nanoparticles as biosensor probes and antibody-functionalized improved upconversion nanoparticles as signal probes to develop an ultrasensitive fluorescence biosensor, which showed the LOD of 0.001 ng/mL and detection linear range of 0.001-0.1 ng/mL for AFB 1 and DON in peanut oils [102].…”

Section: Other Biosensor Techniques For Multiplex Mycotoxin Analysismentioning

confidence: 99%

High Throughput Detection Methods for Multiplex Mycotoxins

Li¹,

Deng²,

Liu³

et al. 2017

Toxicol Open Access

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Mycotoxins are toxic low-molecular-weight compounds which produced by the metabolism of certain fungi species. Due to their toxicity, chemistry stability, diversity and co-occurrence in agriculture products, it is urgently needed to develop the rapid, simple and effective detection technique methods to monitor and prevent mycotoxin contamination in whole food chain. This article gives a review about high throughput detection methods for multiplex mycotoxins which mainly includes chromatographic instrument techniques, microarray chip, suspension array, lateral flow biosensors, surface plasmon resonance (SPR) and nanoparticle-based biosensors. The advantages and disadvantages and the key steps of them have been discussed. The insight and evaluation of the technique progress were given, which would be helpful to further develop this filed.

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Section: Other Biosensor Techniques For Multiplex Mycotoxin Analysismentioning

confidence: 99%

High Throughput Detection Methods for Multiplex Mycotoxins

Li¹,

Deng²,

Liu³

et al. 2017

Toxicol Open Access

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“…The simultaneous determination of two analytes in a single well by QDs-based FLISA was already described in our previous publication [35]. This approach is exclusively possible if conjugates are labeled with particles that luminescent in different part of spectrum.…”

Section: Development Of the Slqds-based Flisamentioning

confidence: 99%

“…Fig. 3 presents the comparison of the calibration curves for determination of ZEN and AfB1 by QDs- [35] and SLQDs-labeled multiplex FLISA. Due to the higher photoluminescence of SLQDs compared with QDs the lower concentration of specific antibodies was used in the same kind of multiplex based on QDs-detection, and it resulted in the significant increase of the assay sensitivity (Table 1) …”

Section: Development Of the Slqds-based Flisamentioning

confidence: 99%

“…The multiplex procedure was based on simultaneous determination of two analytes (mycotoxins zearalenone (ZEN) and aflatoxin B1 (AfB1)) in the single well of microtiter plate by co-immobilization of two specific antibodies there. This technique was the first time described by us using QDs-labeled conjugates [35]. Replacement of the label for the SLQDs resulted in the significant increase of assay sensitivity.…”

Section: Introductionmentioning

confidence: 99%

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Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay

Beloglazova¹,

Goryacheva²,

Speranskaya³

et al. 2015

Talanta

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In: TALANTA, 134, 120-125, 2015. To refer to or to cite this work, please use the citation to the published version:Beloglazova N., Goryacheva O., Speranskaya E., Aubert T., Shmelin P. , Kurbangaleev V., Goryacheva I.Y., De Saeger S. (2015). Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay. TALANTA, 134,[120][121][122][123][124][125] AbstractThis manuscript describes synthesis and followed application of silica-coated liposomes loaded with quantum dots as a perspective label for immunoaasay. The hollow spherical structure of liposomes makes them an attractive package material for encapsulation of multiple water-insoluble quantum dots and amplifying the analytical signal. Silica coverage ensures the stability of the loaded liposomes against fusion and internal leakage during storage, transporting, application and also provides groups for bioconugation. For the first time these nanostructures were employed for the sensitive multiplex immunochemical determination of two analytes. As a model system mycotoxins zearalenone and aflatoxin B1 were detected in cereals. For simplification of multiassay results' evaluation the silanized liposomed loaded with QDs of different colors were used. The IC 50 values for the simultaneous determination of zearalenone and aflatoxin B1 were 16.2 and 18 µg kg -1 for zearalenone and 2.2 and 2.6 µg kg -1 for aflatoxin B1 in wheat and maize, respectively. As confirmatory method, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used.

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“…These advantages have been widely applied for the multiplexed simultaneous detection of different targets [2][3][4][5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Multicolored silica coated CdSe core/shell quantum dots

Goftman

Маркин

Saeger

et al. 2016

Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and L

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Silanization is a convenient route to provide water-solubility to the quantum dots (QDs) with different structure. Green, orange and red emitting CdSe-based QDs were synthesized by varying of number and material of wider-band gap shells and fluorescent properties of QDs were characterized before and after silanization. It was shown that structure of the QD influences on the quantum yield of the silanized QDs: the better CdSe core is protected with wider-band gap semiconductor shells, the more fluorescence properties remain after silica coated QD possess. Hence silica coated QDs have a great perspectives for the multiplex analysis

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Novel multiplex fluorescent immunoassays based on quantum dot nanolabels for mycotoxins determination

Cited by 119 publications

References 43 publications

High Throughput Detection Methods for Multiplex Mycotoxins

High Throughput Detection Methods for Multiplex Mycotoxins

Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay

Multicolored silica coated CdSe core/shell quantum dots

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