Novel monitoring strategies for xenoestrogens

Oosterkamp, A. J.; Hock, Bertold; Seifert, Martin; Irth, H.

doi:10.1016/s0165-9936(97)00099-x

Cited by 46 publications

(29 citation statements)

References 43 publications

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“…In addition, cross reactivity of a similarly derived mouse antibody toward hemolymph samples was verified using a competitive Ž . enzyme-linked immunoassay Oosterkamp et al, 1997 . Trout serum Vg was used as the competitive antigen Ž .…”

Section: žmentioning

confidence: 99%

Determination of vitellogenin-like properties inMya arenaria hemolymph (Saguenay Fjord, Canada): A potential biomarker for endocrine disruption

et al. 1999

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The level of vitellogenin‐like (Vg‐like) proteins in the hemolymph of the soft‐shell clam (Mya arenaria) was indirectly determined using an alkali‐labile phosphate (ALP) measurement assay. Biochemical characterization of hemolymph from actively reproducing clams showed the presence of Zn, Ca and of substantial amounts of ALP, all of which are indicative of fish serum vitellogenin, reported to be a Zn and Ca containing glycolipophosphoprotein. ALP assays undertaken on different phosphate‐containing compounds (phosphate‐buffer saline, diphospholipids and 12,000×g supernatants of Vg‐rich trout ovary homogenates) showed little interference by the first two substrates and confirmed the specificity of the ALP assay as an indirect method of determining Vg‐like proteins in clam hemolymph. Moreover, clams injected with 17β‐estradiol and two known xenoestrogens (nonylphenol and pentachlorophenol) significantly induced hemolymph ALP levels. Field work revealed significant differences in hemolymph ALP levels from clams collected at specific intertidal stations of the Saguenay Fjord during temporal (May to October) and spatial (June) surveys in 1997, which appear to indicate that (anti‐)estrogenic chemicals may be present in the Saguenay Fjord. Our preliminary results thus show that this simple and rapid ALP assay holds promise as a biomarker of estrogen disruption with molluscan shellfish as bioindicators. ©1999 John Wiley & Sons, Inc. Environ Toxicol 14: 455–465, 1999

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Section: žmentioning

confidence: 99%

Determination of vitellogenin-like properties inMya arenaria hemolymph (Saguenay Fjord, Canada): A potential biomarker for endocrine disruption

et al. 1999

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“…Based on estrogen receptors, several biosensors have been developed which provide significant and useful information about estrogenic potency of the sample. The advantage of receptor assays is that they are quite simple to perform and allow the identification of all endocrine disruptors that act through the corresponding receptor [16]. These biosensors constitute a similar approach as that described above for toxicity biosensors, where effects as a general parameter and not a specific substance are monitored.…”

Section: Chlamydia Trachomatismentioning

confidence: 99%

Biosensors for environmental applications: Future development trends

Rodríguez-Mozaz

Marco

Alda

et al. 2004

Pure and Applied Chemistry

197

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Biosensors can be excellent analytical tools for monitoring programs working to implement legislation. In this article, biosensors for environmental analysis and monitoring are extensively reviewed. Examples of biosensors for the most important families of environmental pollutants, including some commercial devices, are presented. Finally, future trends in biosensor development are discussed. In this context, bioelectronics, nanotechnology, miniaturization, and especially biotechnology seem to be growing areas that will have a marked influence on the development of new biosensing strategies in the next future.

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“…1. A number of different techniques have been previously employed in the separation of steroids; these include immunoassays [1], chromatographic [2,3] and CE [4] techniques from a variety of sample matrices. An LC technique does not require compounds to be volatile or to have high molecular weight like those requiring GS-MS determination.…”

Section: Introductionmentioning

confidence: 99%

Determination of association constants of inclusion complexes of steroid hormones and cyclodextrins from their electrophoretic mobility

Shakalisava

Regan

2006

Electrophoresis

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CE estimation of the association constants of several steroid hormones with beta-CD and gamma-CD and their hydroxypropyl derivative is presented. Estriol, 17beta-estradiol, ethynylestradiol, estrone, progesterone, mestranol and norethindrone are among the target analytes. The calculation of the cyclodextrin:analyte association constants were performed from the electrophoretic mobility values of steroids at different concentration of CDs in the run buffer. The reliability of the final data was ensured by employing three different linearisation plots (double reciprocal fit, Y-reciprocal fit and X-reciprocal fit). The highest inclusion affinity of target analytes was observed towards gamma-CD and its hydroxypropyl derivative, which is demonstrated by high association constant values and corresponding good linearity of the plots. The affinity of steroids towards a particular CD type based on physical and structural characteristics is explored.

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Novel monitoring strategies for xenoestrogens

Cited by 46 publications

References 43 publications

Determination of vitellogenin-like properties inMya arenaria hemolymph (Saguenay Fjord, Canada): A potential biomarker for endocrine disruption

Determination of vitellogenin-like properties inMya arenaria hemolymph (Saguenay Fjord, Canada): A potential biomarker for endocrine disruption

Biosensors for environmental applications: Future development trends

Determination of association constants of inclusion complexes of steroid hormones and cyclodextrins from their electrophoretic mobility

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