Novel microparticulate systems for the vaginal delivery of nystatin: Development and characterization

Martín-Villena, María J.; Fernández-Campos, Francisco; Calpena, Ana Cristina; Febrer, Nuria Bozal-de; Ruiz-Martínez, María Adolfina; Clares, Beatriz

doi:10.1016/j.carbpol.2013.01.005

Cited by 59 publications

(26 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Microencapsulation was proposed as a method to protect and ensure drug's delivery in specific tracts [19]. Nowadays, microencapsulation and freeze drying represent appropriate strategies to maintain the viability and stability of probiotics for use in both food and pharmaceutical technologies [24,25].…”

Section: Resultsmentioning

confidence: 99%

“…The microcapsules help fragile and sensitive materials to survive processing and packaging conditions and stabilize the shelf life of the active ingredient [26]. Also, the mucoadhesive properties of selected polymers promote intimate contact between the pharmaceutical form and the vaginal tissue and prolong the residence-time at the administration site [19]. Therefore, in this work the microencapsulation of bovine vaginal probiotics in alginate-MCs was studied.…”

Section: Resultsmentioning

confidence: 99%

“…Extrusion or emulsification techniques are usually used to obtain alginate beads [18]. This polymer possesses mucoadhesive properties and therefore was used for drugs release in human vagina [19].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microencapsulation of Bovine Vaginal Lactobacilli in Alginate Using Emulsion-gelation: Freeze-drying, Storage and Antimicrobial Activity

CE¹,

A²,

C³

2015

J Bioprocess Biotech

View full text Add to dashboard Cite

Probiotics containing autochthonous Lactic Acid Bacteria (LAB) as local treatment in the Bovine Reproductive Tract (BRT) was proposed as a sustainable alternative to prevent outcome of pathogens colonization in the postpartum uterus in cows. Microencapsulation of these LABs could improve their survival during stressing conditions and promote the intimate contact between the veterinarian form and the vaginal mucosa. In this work, emulsion-ionic gelation technique was applied to encapsulate bovine LAB strains in an alginate (3%) matrix. Optical and scanning electron microscopic evaluations showed spheroidal particles (12-48 µm) with a fully charge of LAB; the average load ranged 8.98 ± 0.15 to 8.06 ± 0.21 log CFU/g. The microencapsulated lactic acid bacteria (LAB-MCs) stability was evaluated during lyophilization [in Skim Milk (SM), or Neutral Distilled Water (NDW)] and storage (at 4°C up to 90 days). SM represented a significant high protection to the lyophilization. Also, the alginate microencapsulation improved the LAB strain resistance when freeze-dried in water, comparing to known sensibility of LAB free cells. L. gasseri CRL 1412 showed similar resistance in both, NDW (0.70 ± 0.05) and SM (0.72 ± 0.05); and their microcapsules (MCs) exhibited antagonistic activity against E. coli 99/14 (pathogen from Bovine metritis) when cultured together; contrary, in co-culture with empty-MCs no inhibition was observed. To evaluate the microencapsulation process, different parameters were estimated: Encapsulation Factor (EF) (ranged between 0.76 ± 0.03 and 0.85 ± 0.08) and Encapsulating Efficiency (EE) (average EE%=75%) none significant differences (LSD-Fisher test, P<0.05) were observed between LAB strains. Taking account the weight of the materials, the calculated average yield was 50.5%. The standardized encapsulation conditions allowed selected L. gasseri CRL 1412-MCs as potential systems to be included in formulations to restore vaginal microbiota to prevent metritis in cows.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Microencapsulation of Bovine Vaginal Lactobacilli in Alginate Using Emulsion-gelation: Freeze-drying, Storage and Antimicrobial Activity

CE¹,

A²,

C³

2015

J Bioprocess Biotech

View full text Add to dashboard Cite

show abstract

“…imizadole and nystatin) microspheres [163][164][165] and nanospheres [166]. This strategy aims at improving drug retention in the vagina, due to their mucoadhesive properties while allowing for controlled drug release with no loss or eventually enhancement of anti-Candida activity [164].…”

Section: Several Authors Used Mucoadhesive Polymers Such As Eudragit®mentioning

confidence: 99%

New strategies for local treatment of vaginal infections

Palmeira‐de‐Oliveira

Martinez-de-Oliveira

2015

Advanced Drug Delivery Reviews

148

View full text Add to dashboard Cite

“…Microparticulate drug delivery systems—multiunit carriers composed of spherical particles with average diameter 1–500 μm—have gained particular interest in the pharmaceutical field, due to their ability to assure prolonged drug release profile, providing stability of labile substances or reducing toxicity of active agents [1,2]. In addition, a large surface area of the microparticles enables assuring more uniform drug absorption, which might be favorable, especially with respect to topical delivery vehicles [3].…”

Section: Introductionmentioning

confidence: 99%

Novel Spray Dried Glycerol 2-Phosphate Cross-Linked Chitosan Microparticulate Vaginal Delivery System—Development, Characterization and Cytotoxicity Studies

et al. 2016

View full text Add to dashboard Cite

Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using glycerol 2-phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of glycerol 2-phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the glycerol 2-phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.

show abstract

Novel microparticulate systems for the vaginal delivery of nystatin: Development and characterization

Cited by 59 publications

References 33 publications

Microencapsulation of Bovine Vaginal Lactobacilli in Alginate Using Emulsion-gelation: Freeze-drying, Storage and Antimicrobial Activity

Microencapsulation of Bovine Vaginal Lactobacilli in Alginate Using Emulsion-gelation: Freeze-drying, Storage and Antimicrobial Activity

New strategies for local treatment of vaginal infections

Novel Spray Dried Glycerol 2-Phosphate Cross-Linked Chitosan Microparticulate Vaginal Delivery System—Development, Characterization and Cytotoxicity Studies

Contact Info

Product

Resources

About