Novel methylcellulose-immobilized cation-exchange precolumn for on-line enrichment of cationic drugs in plasma

Yamamoto, Eiichi; Sakaguchi, Takahisa; Kajima, Takashi; Mano, Nariyasu; Asakawa, Naoki

doi:10.1016/j.jchromb.2004.04.028

Cited by 29 publications

(12 citation statements)

References 17 publications

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“…We have also reported the use of MC-strong cation-exchanger (MC-SCX) for the analysis of cationic drugs and metabolites in plasma and in bile [19][20][21]. These columns allow for an automated analysis of small analytes in plasma that is rapid, simple, and accurate.…”

Section: Introductionmentioning

confidence: 99%

Sensitive determination of aspirin and its metabolites in plasma by LC–UV using on-line solid-phase extraction with methylcellulose-immobilized anion-exchange restricted access media

Yamamoto

Takakuwa

Kato

et al. 2007

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Sensitive determination of aspirin and its metabolites in plasma by LC–UV using on-line solid-phase extraction with methylcellulose-immobilized anion-exchange restricted access media

Yamamoto

Takakuwa

Kato

et al. 2007

Journal of Chromatography B

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“…The relationship between the amount of imipramine injected on the heat-treated Fe(III)-silica gel and the peak area was examined. Under both acidic and neutral loading solvent conditions, the response was almost linear (r = 0.995) up to an injection amount of 25 g. The capacity of the heat-treated Fe(III)-silica gel was equivalent to a sorbent currently available [43].…”

Section: Capacity Of Maqc Columnmentioning

confidence: 96%

Selective retention of basic compounds by metal aquo-ion affinity chromatography

Asakawa¹,

Yamamoto

Asakawa

2014

J. Sep. Science

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A novel metal aquo-ion affinity chromatography has been developed for the analysis of basic compounds using heat-treated silica gel containing hydrated metal cations (metal aquo-ions) as the packing material. The packing materials of the metal aquo-ion affinity chromatography were prepared by the immobilization of a single metal component such as Fe(III), Al(III), Ag(I), and Ni(II) on silica gel followed by extensive heat treatment. The immobilized metals form aquo-ions to present cation-exchange ability for basic analytes and the cation-exchange ability for basic analytes depends on pKa of the immobilized metal species. In the present study, to evaluate the retention characteristics of metal aquo-ion affinity chromatography, the on-line solid-phase extraction of drugs was investigated. Obtained data clearly evidence the selective retention capability of metal aquo-ion affinity chromatography for basic analytes with sufficient capacity.

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“…As a result, the target compound in the phosphate buffer-containing organic solvent was diluted ten-fold by aqueous solution prior to delivery to the trap column. Therefore, the efficiency of sample trapping or focusing on the column was significantly increased [10][11][12]. Since aqueous solution is used to trap the target compound, phosphate buffer can easily be removed and shunted to waste.…”

Section: Instrumentation and Conditionsmentioning

confidence: 99%

On-Line Sample Treatment LC System for MS Compatibility

Hashi

Yao

et al. 2008

Chroma

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In the present work, a new liquid chromatography-mass spectrometry (LC-MS) system with on-line pretreatment using column switching and a dilution function was developed. This system can be used under conventional high-performance liquid chromatography (LC) separation conditions, including mobile phases containing phosphate buffer. The built-in autodilution function greatly improves the trapping efficiency for target compounds, followed by desaltation that optimizes the ionization conditions for MS analysis. This fully automated two-dimensional LC system interfaced with mass spectrometry provided a powerful tool for the determination of impurity profiles in pharmaceutical research and the identification of traditional Chinese medicine in natural products.

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Novel methylcellulose-immobilized cation-exchange precolumn for on-line enrichment of cationic drugs in plasma

Cited by 29 publications

References 17 publications

Sensitive determination of aspirin and its metabolites in plasma by LC–UV using on-line solid-phase extraction with methylcellulose-immobilized anion-exchange restricted access media

Sensitive determination of aspirin and its metabolites in plasma by LC–UV using on-line solid-phase extraction with methylcellulose-immobilized anion-exchange restricted access media

Selective retention of basic compounds by metal aquo-ion affinity chromatography

On-Line Sample Treatment LC System for MS Compatibility

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