Selective retention of basic compounds by metal aquo-ion affinity chromatography

Asakawa, Yoshiki; Yamamoto, Eiichi; Asakawa, Naoki

doi:10.1002/jssc.201400296

Cited by 2 publications

(1 citation statement)

References 40 publications

(55 reference statements)

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“…In addition, surfactants tend to stick strongly to the stationary phase, which makes it difficult to recover the column [5]. Cation-exchange chromatography has also been applied for the separation of highly polar basic compounds [6][7][8], while it is not compatible with mass spectrometric detection because of using the mobile phase with high concentration of salt. HILIC is another approach for the analysis of basic compounds [9], but the use of a high concentration of organic solvent is not environmentally friendly, which is a detriment to dissolve the high polar basic compounds.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a stationary phase based on silica modified with branched octadecyl groups by Michael addition and photoinduced thiol-yne click chemistry for the separation of basic compounds

Huang

Wang

et al. 2016

J. Sep. Science

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A novel silica-based stationary phase with branched octadecyl groups was prepared by the sequential employment of the Michael addition reaction and photoinduced thiol-yne click chemistry with 3-aminopropyl-functionalized silica microspheres as the initial material. The resulting stationary phase denoted as SiO2 -N(C18)4 was characterized by elemental analysis, FTIR spectroscopy and Raman spectroscopy, demonstrating the existence of branched octadecyl groups in silica microspheres. The separations of benzene homologous compounds, acid compounds and amine analogues were conducted, demonstrating mixed-mode separation mechanism on SiO2 -N(C18)4 . Baseline separation of basic drugs mixture was acquired with the mobile phase of acetonitrile/H2 O (5%, v/v). SiO2 -N(C18)4 was further applied to separate Corydalis yanhusuo Wang water extracts, and more baseline separation peaks were obtained for SiO2 -N(C18)4 than those on Atlantis dC18 column. It can be expected that this new silica-based stationary phase will exhibit great potential in the analysis of basic compounds.

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Section: Introductionmentioning

confidence: 99%

Synthesis of a stationary phase based on silica modified with branched octadecyl groups by Michael addition and photoinduced thiol-yne click chemistry for the separation of basic compounds

Huang

Wang

et al. 2016

J. Sep. Science

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Evaluation and optimization of the metal‐binding properties of a complex ligand for immobilized metal affinity chromatography

Chen

et al. 2016

J of Separation Science

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The simultaneous determination of two binding parameters for metal ions on an immobilized metal affinity chromatography column was performed by frontal chromatography. In this study, the binding parameters of Cu(2+) to l-glutamic acid were measured, the metal ion-binding characteristics of the complex ligand were evaluated. The linear correlation coefficients were all greater than 99%, and the relative standard deviations of two binding parameters were 0.58 and 0.059%, respectively. The experiments proved that the frontal chromatography method was accurate, reproducible, and could be used to determine the metal-binding parameters of the affinity column. The effects of buffer pH, type, and concentration on binding parameters were explored by uniform design experiment. Regression, matching and residual analyses of the models were performed. Meanwhile, the optimum-binding conditions of Cu(2+) on the l-glutamic acid-silica column were obtained. Under these binding conditions, observations and regression values of two parameters were similar, and the observation values were the best. The results demonstrated that high intensity metal affinity column could be effectively prepared by measuring and evaluating binding parameters using frontal chromatography combined with a uniform design experiment. The present work provided a new mode for evaluating and preparing immobilized metal affinity column with good metal-binding behaviors.

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Selective retention of basic compounds by metal aquo-ion affinity chromatography

Cited by 2 publications

References 40 publications

Synthesis of a stationary phase based on silica modified with branched octadecyl groups by Michael addition and photoinduced thiol-yne click chemistry for the separation of basic compounds

Synthesis of a stationary phase based on silica modified with branched octadecyl groups by Michael addition and photoinduced thiol-yne click chemistry for the separation of basic compounds

Evaluation and optimization of the metal‐binding properties of a complex ligand for immobilized metal affinity chromatography

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