Novel Methodology for Fabrication of Tissue-Engineered Tubular Constructs Using Magnetite Nanoparticles and Magnetic Force

Abstract

Novel technologies for creating three-dimensional constructs with complex shapes would be highly useful in tissue engineering. In the present study, tubular structures were constructed using magnetic force. Magnetite nanoparticles in cationic liposomes were taken up by target cells. The magnetically labeled cells were seeded onto ultralow-attachment plates, and a magnet was placed under the wells. After 24 h of culture, the magnetically labeled cells formed a cell sheet. Subsequently, when a cylindrical magnet was rolled onto the cell sheet, the cell sheet was attracted to the magnet and formed a tube around it. The magnet was then removed, leaving behind a tubular structure. Two types of tissue were used to create tubular structures: urinary tissue, consisting of a monotypic urothelial cell layer; and vascular tissue, consisting of heterotypic layers of endothelial cells, smooth muscle cells, and fibroblasts. The present results suggest that this novel methodology using magnetite nanoparticles and magnetic force, which we have termed "magnetic force-based tissue engineering" (Mag-TE), is a promising approach to constructing tissue-engineered tubular structures.

Nano-Engineered Biomaterials for Tissue Regeneration: What Has Been Achieved So Far?

“…Iron oxide nanoparticles (IONP) occupy a special place in cardiovascular therapies as diagnosis agents; however, they have also recently sparked interest in the field of regenerative therapies for cardiac tissue by allowing the manipulation of cells containing IONP with an external applied magnetic force (Ito et al, 2005;Cheng et al, 2010;Souza et al, 2010). Several superparamagnetic IONP formulations (a.k.a.…”

“…This magnetic targeting technique was able to increase retention of CDCs in the infarct region, which lead to improve cardiac function, reduced ventricular remodeling, and reduced infarct expansion post-MI (Cheng et al, 2010). In tissue engineering applications, magnetic force has been used to successfully manipulate IONP labeled cells in culture to create 3D tissue arrangements, including 3D small-diameter vascular grafts with three layers of cells containing IONP (endothelial, smooth muscle, and fibroblast cell layers) (Ito et al, 2005;Souza et al, 2010). A novel application of IONP by (Han et al, 2015) was to enhance the cardiac lineage differentiation of mesenchymal stem cells (MSCs) prior to delivery to the infarct region post-MI in an effort to enhance cardiomyocytes regeneration.…”

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

“…18,19 Array patterns of microparticles or cells have been utilized in the design of biosensors, as scaffolds for patterning proteins, and in cell cultivation. In recent years, various techniques for manipulating bioparticles on chips have been developed using hydrodynamics, 1,73 magnetic forces, [74][75][76][77][78][79][80] and DEP. 16,17,81-85 DEP was first described by Pohl as the motion of dielectric particles under the influence of a non-uniform electric filed.…”

De la cellule au tissu : le magnétisme auxiliaire de la biomédecine

“…Enfi n, il devient possible de fabriquer un tissu mêlant plusieurs types cellulaires, comme l'on fait Ito et al Cette équipe japonaise a également utilisé la technologie magnétique pour construire in vitro des feuillets cellulaires [5]. Par superposition de différents feuillets de cellules endothé-liales, de cellules musculaires lisses et de fi broblastes*, ils sont parvenus à reconstituer un vaisseau sanguin, possédant les caracté-ristiques physiologiques requises (résis-tance hydrodynamique, faible risque de thrombose...) [6]. C'est la première étape vers la construction in vitro d'une architecture multicellulaire complexe.…”