2020
DOI: 10.1186/s13568-020-01111-6
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Novel method to achieve crystallinity of calcite by Bacillus subtilis in coupled and non-coupled calcium-carbon sources

Abstract: Bacteria mineralization is a promising biotechnological approach to apply in biomaterials development. In this investigation, we demonstrate that Bacillus subtilis 168 induces and influences CaCO3 composites precipitation. Crystals were formed in calcium-carbon non-coupled (glycerol + CaCl2, GLY; or glucose + CaCl2, GLC) and coupled (calcium lactate, LAC; or calcium acetate, ACE) agar-sources, only maintaining the same Ca2+ concentration. The mineralized colonies showed variations in morphology, size, and crys… Show more

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Cited by 12 publications
(8 citation statements)
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“…Silica was present in both glassy amorphous and crystalline forms. The peaks at 23°, 29°, 36°, and 48° are due to the calcite phase as a major mineral in the ISA [18], while the small peaks at 33° and 35° are due to the maghemite and magnetite in the ISA. The existence of small, broader, and overlapping reflections in the diffraction pattern of ISA indicates a low-order structure (due to amorphous carbon in ISA).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Silica was present in both glassy amorphous and crystalline forms. The peaks at 23°, 29°, 36°, and 48° are due to the calcite phase as a major mineral in the ISA [18], while the small peaks at 33° and 35° are due to the maghemite and magnetite in the ISA. The existence of small, broader, and overlapping reflections in the diffraction pattern of ISA indicates a low-order structure (due to amorphous carbon in ISA).…”
Section: Resultsmentioning
confidence: 99%
“…A decrease in various peak intensities shows the addition of some material that is absorbing X-rays. Additionally, the sintering process has changed the material composition as a function of temperature [18, 19].…”
Section: Resultsmentioning
confidence: 99%
“…[193] For instance, Bacillus subtilis induces and influences CaCO 3 formation. [194] Moreover, high mechanical stress resistant vaterite is produced by bacteria. [195] However, extended in vitro and in vivo studies are yet to be implemented to address the possible safety issues of bacterial CaCO 3 and unravel their biocompatibility properties.…”
Section: Bioceramicsmentioning
confidence: 99%
“…[29,71,72] Nacre enhances tissue growth and bone tissue bonding. It also has excellent mechanical properties, such as Young modulus (30-40 GPa) and resistance to failure (185)(186)(187)(188)(189)(190)(191)(192)(193)(194)(195)(196)(197)(198)(199)(200), that are similar to those of human bones (20 GPa and 140 MPa, respectively). [72,197] For instance, abalone nacre is a highly ordered structure with extraordinary toughness.…”
Section: Bioceramicsmentioning
confidence: 99%
“…Since then, the importance of cyanobacteria in carbonation has been extensively investigated, with particular regards to the molecular mechanisms driving calcite precipitation in Synechocystis spp and Synecococchus spp (Lamérand et al, 2022; Görgen et al, 2021). In the course of the years Bacillus subtilis, Pseudomonas nitroreducens and Bacillus licheniformis, Bacillus megaterium, Sporosarcina pasteurii ) (Ferral-Pérez et al, 2020; Rajasekar et al, 2021, Jarwar et al 2022) became the most studied bacteria, and widely used in practical applications as soil consolidation and concreate healing, due to their ability to produce high amount of calcite within a short period of time (Bang et al 2001; Dhami et al, 2013; Wei et al, 2015; Rajasekar et al, 2021).…”
Section: Introductionmentioning
confidence: 99%