Novel method for studying plasmid transfer in undisturbed river epilithon

Bale, M. J.; Day, Martin J.; Fry, John C.

doi:10.1128/aem.54.11.2756-2758.1988

Cited by 77 publications

(47 citation statements)

References 10 publications

(13 reference statements)

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“…The capture of plasmids directly into recipients is termed exogenous plasmid isolation and enables plasmid isolation independently of the cultivability of their original hosts. Plasmids can be captured either in biparental or triparental matings (Bale et al, 1988;Hill et al, 1992;see Fig. 2a, b).…”

Section: Strategies To Detect and To Capture Conjugative Plasmids Fromentioning

confidence: 99%

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Plasmids foster diversification and adaptation of bacterial populations in soil

2012

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It is increasingly being recognized that the transfer of conjugative plasmids across species boundaries plays a vital role in the adaptability of bacterial populations in soil. There are specific driving forces and constraints of plasmid transfer within bacterial communities in soils. Plasmid-mediated genetic variation allows bacteria to respond rapidly with adaptive responses to challenges such as irregular antibiotic or metal concentrations, or opportunities such as the utilization of xenobiotic compounds. Cultivation-independent detection and capture of plasmids from soil bacteria, and complete sequencing have provided new insights into the role and ecology of plasmids. Broad host range plasmids such as those belonging to IncP-1 transfer a wealth of accessory functions which are carried by similar plasmid backbones. Plasmids with a narrower host range can be more specifically adapted to particular species and often transfer genes which complement chromosomally encoded functions. Plasmids seem to be an ancient and successful strategy to ensure survival of a soil population in spatial and temporal heterogeneous conditions with various environmental stresses or opportunities that occur irregularly or as a novel challenge in soil.

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Section: Strategies To Detect and To Capture Conjugative Plasmids Fromentioning

confidence: 99%

“…Exogenous isolation of plasmids directly from soil bacteria into labeled recipients in (a) biparental and (b) triparental matings, modified fromBale et al (1988) andHill et al (1992). Adapted by L. Bandounas.…”

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Plasmids foster diversification and adaptation of bacterial populations in soil

2012

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“…Exogenous plasmid isolations were essentially performed as described by Bale et al [22] and Hill et al [23]. Recipient strains were grown in 5 ml Luria broth (LB) supplemented with rifampicin (50 mg l 31 ) at 28 ‡C for approximately 20 h. Cells were harvested by centrifugation for 5 min, 4000Ug at room temperature.…”

Section: Exogenous Isolation Of Mobile Genetic Elements Carrying Gm Rmentioning

confidence: 99%

Gentamicin resistance genes in environmental bacteria: prevalence and transfer

Heuer¹

2002

FEMS Microbiology Ecology

111

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A comprehensive multiphasic survey of the prevalence and transfer of gentamicin resistance (Gm r ) genes in different non-clinical environments has been performed. We were interested to find out whether Gm r genes described from clinical isolates can be detected in different environmental habitats and whether hot spots can be identified. Furthermore, this study aimed to evaluate the impact of selective pressure on the abundance and mobility of resistance genes. The study included samples from soils, rhizospheres, piggery manure, faeces from cattle, laying and broiler chickens, municipal and hospital sewage water, and coastal water. Six clusters of genes coding for Gmmodifying enzymes (aac(3)-I, aac(3)-II/VI, aac(3)-III/IV, aac(6P)-II/Ib, ant(2Q)-I, aph(2Q)-I) were identified based on a database comparison and primer systems for each gene cluster were developed. Gm-resistant bacteria isolated from the different environments had a different taxonomic composition. In only 34 of 207 isolates, mainly originating from sewage, faeces and coastal water polluted with wastewater, were known Gm r genes corresponding to five of the six clusters detected. The strains belonged to genera in which the genes had previously been detected (Enterobacteriaceae, Pseudomonas, Acinetobacter) but also to phylogenetically distant bacteria, such as members of the CFB group, K-and L-Proteobacteria. Gm r genes located on mobile genetic elements (MGE) could be captured in exogenous isolations into recipients belonging to K-, L-and Q-Proteobacteria from all environments except for soil. A high proportion of the MGE, conferring Gm resistance isolated from sewage, were identified as IncPL plasmids. Molecular detection of Gm r genes, and broad host range plasmidspecific sequences (IncP-1, IncN, IncW and IncQ) in environmental DNA indicated a habitat-specific dissemination. A high abundance and diversity of Gm r genes could be shown for samples from faeces (broilers, layers, cattle), from sewage, from seawater, collected close to a wastewater outflow, and from piggery manure. In the latter samples all six clusters of Gm r genes could be detected. The different kinds of selective pressure studied here seemed to enhance the abundance of MGE, while an effect on Gm r genes was not obvious.

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“…One of the most e¡ective methods of obtaining plasmids with transfer pro¢ciency is (bi-or triparental) exogenous isolation. These methods capture transfer-pro¢cient plasmids directly from environmental samples into recipient strains that can be grown in the laboratory [45], and have been successfully applied to soil and phytosphere habitats [32,46]. One plasmid, denoted pIPO2, was shown to self-transfer and mobilize IncQ plasmids to a range of diverse Gram-negative bacteria in the wheat rhizosphere in the ¢eld [46].…”

Section: Plasmids Conferring Gene Mobilizing Capacity Tomentioning

confidence: 99%

The ecology of transfer of mobile genetic elements

VANELSAS¹,

Bailey²

2002

FEMS Microbiology Ecology

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The ecological aspects of the transfer and spread of mobile genetic elements (MGE) are reviewed in the context of the emerging evidence for the dominant role that horizontal gene transfer (HGT) has played in the evolutionary shaping of bacterial communities. Novel tools are described that allow a refined analysis of HGT in natural settings. The occurrence of HGT processes in soil and water, as affected by environmental factors, is then discussed. Examples are provided that illustrate how MGE can influence the behavior of microorganisms in their natural habitats. The occurrence of microorganisms as groups of cells in structured communities, such as those found in biofilms, is used as a framework in order to review the data and pose further questions on the evolutionary role and significance of contemporary gene transfer processes in nature. Selection by the environment is likely to be the dominant force in shaping the genetic make-up of bacterial communities. In fact, selective force can act as an apparent accelerator of gene transfer processes, mainly as a result of the enhancement of survival and persistence of favorably selected products of gene transfer processes (genes, metabolic pathways, microbial cells and communities). However, the current understanding of the triggering and impact of HGT in nature remains limited by our lack of understanding of the very nature and variety of the selective forces that act on microorganisms in situ. Hence, the relevant questions with respect to these triggers acting in natural habitats need to be answered using advanced approaches for studying HGT processes in nature, such as those discussed in this review.

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Novel method for studying plasmid transfer in undisturbed river epilithon

Cited by 77 publications

References 10 publications

Plasmids foster diversification and adaptation of bacterial populations in soil

Plasmids foster diversification and adaptation of bacterial populations in soil

Gentamicin resistance genes in environmental bacteria: prevalence and transfer

The ecology of transfer of mobile genetic elements

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