Novel mesoporous silica nanocarriers containing gold; a rapid diagnostic tool for tuberculosis

Abstract

Tuberculosis (TB) is major health concern and reason of deaths from decades to current date. Even though with a lot of advancements, diagnostic techniques, and discovery of standard antibiotics TB remains crucial challenge and can create worst scenario for human health in near future. Nanoparticles play emerging role in diagnosis and treatment of TB. In this study, we developed mesoporous silica nanoparticles containing gold (MSNs@GNPs) for rapid diagnosis and treatment of TB. The physicochemical characterization revealed effective surface morphology and particles diameter, that is applicable for in vitro applications. The in vitro antimicrobial analysis revealed that the designed MSNs@GNPs has retained significantly lower minimal inhibitory concentration (MIC) values and can effectively demolish mycobacterium tuberculosis (Mtb). Furthermore, the diagnosis efficiency of the MSNs@GNPs was evaluated by calorimetric analysis. Which demonstrates that MSNs@GNPs can be used for rapid diagnosis of the tuberculosis when applied on in vitro culture of the Mtb. The current study needs further verification on human’s clinical samples from tuberculosis patients. However, MSNs@GNPs can be a versatile clinical approach for the rapid diagnosis and clinical treatment of the tuberculosis.

Advances in the fabrication of potential nanomaterials for diagnosis and effective treatment of tuberculosis

“…The fabricated composite showed a dual effect for the rapid diagnosis and treatment of TB, so it is highly recommended for use in clinical in vivo studies. 5 A similar study has recently confirmed the high tendency of silica mesoporous NPs towards high loading efficiency to be one of the best options for drug loading resistance purposes. Ag NPs have proven to have great bactericidal activity.…”

“…4 TB negatively affects the lungs (i.e., pulmonary TB) and the central nervous system (CNS), causing severe meningitis. 5 TB is primarily caused by Mycobacterium tuberculosis, which is a type of bacteria that can be identified as either Grampositive or Gram-negative on a gram stain. 6 Several clinical approaches have reported the diagnosis of TB, encompassing culture of the tracheal aspirate, cerebrospinal fluid (CSF), gastric washings, chest X-ray, and skin examination.…”

Advanced drug delivery and therapeutic strategies for tuberculosis treatment

“…The antibacterial properties were studied using MIC and minimum bactericidal concentration methods [218]. Mesoporous silica nanoparticles (MSNs) containing gold nanoparticles (MSNs@GNP) can inhibit M. tuberculosis growth and produce a synergistic effect against M. tuberculosis, making it safe for TB treatment [219]. Another important application of GNP is in TB diagnosis.…”