Novel Media for Lipid Production of Chlorococcum oleofaciens : A RSM Approach

Pauline, J. Mercy Nisha; Achary, Anant

doi:10.4467/16890027ap.19.003.10834

Cited by 4 publications

(6 citation statements)

References 21 publications

(23 reference statements)

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“…The RSM is an interesting approach for the optimization of biomass and specific metabolites. Over the years, this method has been proven to be an interesting statistical tool for better culture media in strains such as Ankistrodesmus falcatus KJ671624, 48 Auxenochlorella protothecoides , 49 Botryococcus braunii , 24 B. braunii LB572, 50 Chlorella sp., 51 Chlorellaprotothecoides , 52 Chlorella sorokiniana Mb-1, 53 Chlorella vulgaris , 54 C. vulgaris 1803, 55 Chlorococcum oleofaciens , 17 Desmodesmus armtus , 56 Dunaliella salina , 57 C. pyrenoidosa , 58 Scenedesmus sp. ASK22, 59 Scenedesmus obliquus , 60 , 61 and Spirulina sp.…”

Section: Discussionmentioning

confidence: 99%

“…braunii LB572, Chlorella sp., Chlorellaprotothecoides, Chlorella sorokiniana Mb-1, Chlorella vulgaris, C. vulgaris 1803, Chlorococcum oleofaciens, Desmodesmus armtus, Dunaliella salina, C. pyrenoidosa, Scenedesmus sp.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…The production, accumulation, and purity of phycobiliproteins in cyanobacteria are strongly regulated by various factors such as the strain to be used, light intensity and its wavelength, temperature, pH, and media composition. − Generally, algae and cyanobacteria have been isolated and produced on specific culture media composed of inorganic salts . Therefore, it is possible to modulate (or artificially adjust) the final concentration of PBPs.…”

Section: Introductionmentioning

confidence: 99%

“…14−16 Generally, algae and cyanobacteria have been isolated and produced on specific culture media composed of inorganic salts. 17 Therefore, it is possible to modulate (or artificially adjust) the final concentration of PBPs. Media composition plays an essential role in microalgal and cyanobacterial growth and byproduct (lipids, carotenes, etc.)…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

et al. 2021

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Phycobiliproteins (PBPs) are a group of brilliant pigment proteins found in cyanobacteria and red algae; their synthesis and accumulation depend on several factors such as the type of strain employed, nutrient concentration, light intensity, light regimes, and others. This study evaluates the effect of macronutrients (citrate buffer, NaNO 3 , K 2 HPO 4 , MgSO 4 , CaCl 2 , Na 2 CO 3 , and EDTA) and the concentration of trace metals in BG-11 media on the accumulation of PBPs in a thermotolerant strain of Oscillatoria sp. The strain was grown in BG-11 media at 28 °C with a light:dark cycle of 12:12 h at 100 μmol m –2 s –1 for 15 days, and the effect of nutrients was evaluated using a Plackett–Burman Design followed by optimization using a response surface methodology. Results from the concentration of trace metals show that it can be reduced up to half-strength in its initial concentration without affecting both biomass and PBPs. Results from the Plackett–Burman Design revealed that only NaNO 3 , Na 2 CO 3 , and K 2 HPO 4 show a significant increase in PBP production. Optimization employed a central Non-Factorial Response Surface Design with three levels and four factors (3 4 ) using NaNO 3 , Na 2 CO 3 , K 2 HPO 4 , and trace metals as variables, while the other components of BG-11 media (citrate buffer, MgSO 4 , CaCl 2 , and EDTA) were used in half of their initial concentration. Results from the optimization show that interaction between Na 2 CO 3 and K 2 HPO 4 highly increased PBPs’ concentration, with values of 15.21, 3.95, and 1.89 (% w/w), respectively. These results demonstrate that identifying and adjusting the concentration of critical nutrients can increase the concentration of PBPs up to two times for phycocyanin and allophycocyanin while four times for phycoerythrin. Finally, the reduction in non-key nutrients’ concentration will reduce the production costs of colorants at an industrial scale and increase the sustainability of the process.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

et al. 2021

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Evaluation of microalgal strains and microalgal consortium for higher lipid productivity and rich fatty acid profile towards sustainable biodiesel production

Arutselvan

Narchonai

Pugazhendhi

et al. 2021

Bioresource Technology

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Enhancement of Metabolite Production in High-Altitude Microalgal Strains by Optimized C/N/P Ratio

et al. 2022

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This study evaluated the role of C/N/P in the increase in the synthesis of carbohydrates, proteins, and lipids in two high-mountain strains of algae (Chlorella sp. UFPS019 and Desmodesmus sp. UFPS021). Three carbon sources (sodium acetate, sodium carbonate, and sodium bicarbonate), and the sources of nitrogen (NaNO3) and phosphate (KH2PO4 and K2HPO4) were analyzed using a surface response (3 factors, 2 levels). In Chlorella sp. UFPS019, the optimal conditions to enhance the synthesis of carbohydrates were high sodium carbonate content (3.53 g/L), high KH2PO4 and K2HPO4 content (0.06 and 0.14 g/L, respectively), and medium-high NaNO3 (0.1875 g/L). In the case of lipids, a high concentration of sodium acetate (1.19 g/L) coupled with high KH2PO4 and K2HPO4 content (0.056 and 0.131 g/L, respectively) and a low concentration of NaNO3 (0.075 g/L) drastically induced the synthesis of lipids. In the case of Desmodesmus sp. UFPS021, the protein content was increased using high sodium acetate (2 g/L), high KH2PO4 and K2HPO4 content (0.056 and 0.131 g/L, respectively), and high NaNO3 concentration (0.25 g/L). These results demonstrate that the correct adjustment of the C/N/P ratio can enhance the capacity of high-mountain strains of algae to produce high concentrations of carbohydrates, proteins, and lipids.

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Novel Media for Lipid Production of Chlorococcum oleofaciens : A RSM Approach

Cited by 4 publications

References 21 publications

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

Evaluation of microalgal strains and microalgal consortium for higher lipid productivity and rich fatty acid profile towards sustainable biodiesel production

Enhancement of Metabolite Production in High-Altitude Microalgal Strains by Optimized C/N/P Ratio

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