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Highlights Isolation of microalgae from temple ponds for biobutanol production. Phosphorus and Sulphur stress induces starch accumulation in Chlorococcum humicola . 2,4-D induction effects C. humicola growth and increases biomass production. Clostridium acetobutylicum used for fermentation of starch in small scale batch fermenter. Microalgae as third generation substrate for biological production of biobutanol.

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