Novel mechanisms controlling arginine metabolism in Neurospora

Davis, Rowland H.; Weiss, Richard L.

doi:10.1016/0968-0004(88)90050-3

Cited by 22 publications

(8 citation statements)

References 20 publications

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“…VOL. 15,1995 N. CRASSA TRANSLATIONAL CONTROL 5239 on May 13, 2018 by guest http://mcb.asm.org/ (6,41,43,52). In addition to these effects on transcript levels (Fig.…”

Section: Resultsmentioning

confidence: 94%

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Translational Regulation in Response to Changes in Amino Acid Availability in Neurospora crassa

Luo

Freitag

Sachs

1995

Molecular and Cellular Biology

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We examined the regulation of Neurospora crassa arg-2 and cpc-1 in response to amino acid availability. arg-2 encodes the small subunit of arginine-specific carbamoyl phosphate synthetase; it is subject to unique negative regulation by Arg and is positively regulated in response to limitation for many different amino acids through a mechanism known as cross-pathway control. cpc-1 specifies a transcriptional activator important for crosspathway control. Expression of these genes was compared with that of the cytochrome oxidase subunit V gene, cox-5. Analyses of mRNA levels, polypeptide pulse-labeling results, and the distribution of mRNA in polysomes indicated that Arg-specific negative regulation of arg-2 affected the levels of both arg-2 mRNA and arg-2 mRNA translation. Negative translational effects on arg-2 and positive translational effects on cpc-1 were apparent soon after cells were provided with exogenous Arg. In cells limited for His, increased expression of arg-2 and cpc-1, and decreased expression of cox-5, also had translational and transcriptional components. The arg-2 and cpc-1 transcripts contain upstream open reading frames (uORFs), as do their Saccharomyces cerevisiae homologs CPA1 and GCN4. We examined the regulation of arg-2-lacZ reporter genes containing or lacking the uORF start codon; the capacity for arg-2 uORF translation appeared critical for controlling gene expression.

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“…VOL. 15,1995 N. CRASSA TRANSLATIONAL CONTROL 5239 on May 13, 2018 by guest http://mcb.asm.org/ (6,41,43,52). In addition to these effects on transcript levels (Fig.…”

Section: Resultsmentioning

confidence: 94%

“…Control of arg-2 expression regulates the flux of metabolites through the arginine (Arg) biosynthetic pathway under most growth conditions (12,15). Three separate control mechanisms regulate arg-2 expression.…”

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confidence: 99%

Translational Regulation in Response to Changes in Amino Acid Availability in Neurospora crassa

Luo

Freitag

Sachs

1995

Molecular and Cellular Biology

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“…The reason for the decreased ADC activity in greenislands is not known. However, it is known that when most fungi are presented with an exogenous supply of arginine, they will take it up rapidly and store or use it as a nitrogen source (Davis & Weiss, 1988). The possibility that mildew might deplete the arginine concentration in the green-island region, thus depriving ADC of its substrate, is worthy of further investigation.…”

Section: Discussionmentioning

confidence: 99%

Polyamine metabolism in ‘green‐islands’ on powdery mildew‐infected barley leaves: possible interactions with senescence

Coghlan

Walters

1990

New Phytologist

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SUMMARYPolyamine metabolism, lipoxygenase and catalase activities and ethylene production were examined in greenislands induced to form on detached barley leaves infected with the powdery mildew fungus, Erysiphe graminis f. sp. hordei Marchal. Several-fold increases in free polyamines were detected in green-islands, which could not be accounted for by changes in polyamine biosynthetic and degradative enzymes: a small increase in ornithine decarboxylase (ODC) activity, greatly decreased arginine decarboxylase (ADC) activity, virtually unchanged activity of S-adenosylmethionine decarboxylase (AdoMetDC) and greatly increased polyamine oxidase (PAO) activity. Changes in conjugated forms of polyamines suggest that elevated free polyamine concentrations might result from reductions in this pool. Green-island tissues displayed a reduced activity of lipoxygenase, unchanged catalase activity and a greatly lowered rate of ethylene production. Chlorophyll concentration and rates of photosynthesis and dark respiration were slightly increased in these tissues.In senescing regions of infected leaves, there was a small increase in free putrescine and spermine and a decrease in spermidine concentration, while concentrations of conjugated polyamines were substantially reduced. This was accompanied by an overall decrease in ODC and ADC activities and an increased activity of both AdoMetDC and PAO. These regions exhibited lowered lipoxygenase activity, greatly enhanced catalase activity and reduced rates of ethylene production. In addition, senescing regions exhibited a substantial reduction in chlorophyll concentration and the rate of photosynthesis, although dark respiration was similar to control values.These results support the view that green-islands represent areas of the infected leaf where senescence is retarded, biosynthetic activity is retained and in which polyamines may play an important role. On the other hand, although polyamine concentrations were reduced in senescing areas, other changes occurring there suggest that some functional integrity is maintained in that tissue.

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“…Arginine is synthesized partially in mitochondria and partially in cytoplasm ( Fig. 1) (Davis, 1986;Davis and Weiss, 1988). Excess of ornithine and arginine is sequestered in the vacuole (Weiss, 1973).…”

Section: Introductionmentioning

confidence: 99%

Arginine catabolism in Aspergillus nidulans is regulated by the rrmA gene coding for the RNA-binding protein

Olszewska

Krol

Węgleński

et al. 2007

Fungal Genetics and Biology

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Novel mechanisms controlling arginine metabolism in Neurospora

Cited by 22 publications

References 20 publications

Translational Regulation in Response to Changes in Amino Acid Availability in Neurospora crassa

Translational Regulation in Response to Changes in Amino Acid Availability in Neurospora crassa

Polyamine metabolism in ‘green‐islands’ on powdery mildew‐infected barley leaves: possible interactions with senescence

Arginine catabolism in Aspergillus nidulans is regulated by the rrmA gene coding for the RNA-binding protein

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