Novel Mammalian orthorubulavirus 5 Discovered as Accidental Cell Culture Contaminant

Feehan, Brandi; Penin, Aleksey A.; Mukhin, A.N.; Kumar, Deepak; Moskvina, Anna S.; Хаметова, К.М.; Yuzhakov, Anton G.; Musienko, Maria I.; Zaberezhny, Alexey D.; Алипер, Т.И.; Marthaler, Douglas; Alekseev, Konstantin P.

doi:10.3390/v11090777

Cited by 6 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VSV is frequently used as an experimental tool, for example, as a pseudotype virus ( 40 ). Previous studies have also reported possible contamination of PIV5 in cultured cells ( 41 , 42 ). Additionally, it has been reported that KDV RNA might be a contaminant in the nucleic acid extraction kit ( 43 ).…”

Section: Discussionmentioning

confidence: 90%

Hidden Viral Sequences in Public Sequencing Data and Warning for Future Emerging Diseases

Kawasaki

Kojima

Tomonaga

et al. 2021

mBio

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 90%

Hidden Viral Sequences in Public Sequencing Data and Warning for Future Emerging Diseases

Kawasaki

Kojima

Tomonaga

et al. 2021

mBio

View full text Add to dashboard Cite

show abstract

“…These changes are similar to those caused by other animal PIV5 strains (Liu et al., 2015, 2017). It is also clear that the true genetic diversity of PIV5 may be greater than that first realized (Feehan et al., 2019), meaning that low‐level PIV5 circulation in ticks remains a potential risk of infection transmission to humans.…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification and pathogenic characteristics of tick‐derived parainfluenza virus 5 in northeast China

Yang

Jiang

et al. 2022

Transbounding Emerging Dis

View full text Add to dashboard Cite

The number of parainfluenza virus 5 (PIV5) infection cases has increased worldwide over the past six decades; however, factors underlying this increase remain unclear. PIV5 has been emerging or re‐emerging in humans and animal species. To date, no information is yet available regarding PIV5 infection in arthropod ticks. Here, we successfully isolated tick‐derived PIV5 from the Ixodes persulcatus species designated as HLJ/Tick/2019 in Heilongjiang, China. Phylogenetic analysis revealed that the tick‐derived PIV5 is closely related to subclade 2.2.6, which has become the dominant subtype prevalent in dogs, pigs and wildlife across China. Further experiments to understand the importance of this virus as an infectious vector revealed that a ferret animal model experimentally infected with Tick/HLJ/2019 via the oronasal and ocular inoculation routes developed moderate respiratory distress with pneumonia and neurologic tissue damage from inflammation for the first time. Further surveillance of PIV5 in vectors of viral transmission is necessary to enhance our knowledge of its ecology in reservoirs and facilitate the control of re‐emerging diseases.

show abstract

“…VSV is frequently used as an experimental tools; for example, as a pseudotype virus (43). Additionally, previous studies have reported possible contamination of PIV5 in cultured cells (44,45). Therefore, we excluded these viral infections to avoid counting false positives.…”

Section: Discussionmentioning

confidence: 99%

Hidden viral sequences in public sequencing data and warning for future emerging diseases

Kawasaki

Kojima

Tomonaga

et al. 2021

Preprint

View full text Add to dashboard Cite

RNA viruses cause numerous emerging diseases, mostly due to transmission from mammalian and avian reservoirs. Large-scale surveillance of RNA viral infections in these animals is a fundamental step for controlling viral infectious diseases. Metagenomic analysis is a powerful method for virus identification with low bias and has substantially contributed to the discovery of novel viruses. Deep sequencing data have been accumulated in public databases in recent decades; however, only a small number of them have been examined for viral infections. Here, we screened for infections of 33 RNA viral families in publicly available mammalian and avian RNA-seq data and found over 900 hidden viral infections. We also discovered viral sequences in livestock, wild, and experimental animals: hepatovirus in a goat, hepeviruses in blind mole-rats and a galago, astrovirus in macaque monkeys, parechovirus in a cow, pegivirus in tree shrews, and seadornavirus in rats. Some of these viruses were phylogenetically close to human pathogenic viruses, suggesting the potential risk of causing disease in humans upon infection. Furthermore, the infections of five novel viruses were identified in several different individuals, indicating that their infections may have already spread in the natural host population. Our findings demonstrate the reusability of public sequencing data for surveying viral infections and identifying novel viral sequences, presenting a warning about a new threat of viral infectious disease to public health.

show abstract

Novel Mammalian orthorubulavirus 5 Discovered as Accidental Cell Culture Contaminant

Cited by 6 publications

References 11 publications

Hidden Viral Sequences in Public Sequencing Data and Warning for Future Emerging Diseases

Hidden Viral Sequences in Public Sequencing Data and Warning for Future Emerging Diseases

Isolation, identification and pathogenic characteristics of tick‐derived parainfluenza virus 5 in northeast China

Hidden viral sequences in public sequencing data and warning for future emerging diseases

Contact Info

Product

Resources

About