Novel LTCC-potentiometric microfluidic device for biparametric analysis of organic compounds carrying plastic antibodies as ionophores: Application to sulfamethoxazole and trimethoprim

Almeida, S.A.A.; Arasa, E.; Puyol, Mar; Martínez-Cisneros, Cynthia S.; Alonso-Chamarro, Julián; Montenegro, M.C.B.S.M.; Sales, M. Goreti F.

doi:10.1016/j.bios.2011.09.011

Cited by 33 publications

(10 citation statements)

References 19 publications

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“…Electrodes can be deposited onto LTCC using expansion matched metal pastes. In this manner Almeida et al 32 used an LTCC device with potentiometric detection to analyze sulfamethoxazole and trimethoprim in fish farm water. Electrophoretic separations have been demonstrated for phenolic compounds 33, 34 and inorganic ions.…”

Section: Materials For Microfluidicsmentioning

confidence: 99%

Advances in Microfluidic Materials, Functions, Integration, and Applications

2013

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Section: Materials For Microfluidicsmentioning

confidence: 99%

Advances in Microfluidic Materials, Functions, Integration, and Applications

2013

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“…At a first glance, this may seem a similar process to that presented by Almeida et al (2011). However, the chemical path follows a completely different scheme, accounting for the great chemical differences between the silica spheres employed there and the CN used here.…”

Section: Imprinting On Carbon Nanostructuresmentioning

confidence: 57%

“…All these are too expensive or unable to carry out on-site analysis. The potentiometric sensors described so far do not have the necessary LOD for field applications (Almeida et al, 2011(Almeida et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

Optimizing potentiometric ionophore and electrode design for environmental on-site control of antibiotic drugs: Application to sulfamethoxazole

Almeida

Truta

Queirós

et al. 2012

Biosensors and Bioelectronics

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Potentiometric sensors are typically unable to carry out on-site monitoring of environmental drug con-taminants because of their high limits of detection (LODs). Designing a novel ligand material for the target analyte and managing the composition of the internal reference solution have been the strategies employed here to produce for the first time a potentiometric-based direct reading method for an envi-ronmental drug contaminant. This concept has been applied to sulfamethoxazole (SMX), one of the many antibiotics used in aquaculture practices that may occur in environmental waters.The novel ligand has been produced by imprinting SMX on the surface of graphitic carbon nanos-tructures (CN) < 500 nm. The imprinted carbon nanostructures (ICN) were dispersed in plasticizer and entrapped in a PVC matrix that included (or not) a small amount of a lipophilic additive. The mem-brane composition was optimized on solid-contact electrodes, allowing near-Nernstian responses down to 5.2 µg/mL and detecting 1.6 µg/mL. The membranes offered good selectivity against most of the ionic compounds in environmental water.The best membrane cocktail was applied on the smaller end of a 1000 µL micropipette tip made of polypropylene. The tip was then filled with inner reference solution containing SMX and chlorate (as interfering compound). The corresponding concentrations were studied for 1 × 10 −5 to 1 × 10 −10 and 1 × 10 −3 to 1 × 10 −8 mol/L. The best condition allowed the detection of 5.92 ng/L (or 2.SMX for a sub-Nernstian slope of −40.3 mV/decade from 5.0 × 10 −8 to 2.4 × 10 −5 mol/L. The described sensors were found promising devices for field applications. The good selectivity of the sensory materials together with a carefully selected composition for the inner reference solution allowed LODs near the nanomolar range. Both solid-contact and "pipette tip"-based sensors were successfully applied to the analysis of aquaculture waters.

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“…Low-temperature co-fired ceramics (LTCC) were laminar in nature, thus helping in the construction of a three-dimensional device where a stacked or a layered structure could be build. In this manner, Almeida et al (2011) used an LTCC device with potentiometric detection to analyze sulfamethoxazole and trimethoprim in fish farm water. Fakunle and Fritsch (2010) demonstrated that LTCC devices provide low levels of non-specifically adsorbed molecules in immunoassays such as the enzyme-linked immunosorbent assay (ELISA).…”

Section: Traditional Packaging Techniquesmentioning

confidence: 99%