Novel leukaemogenic retroviruses isolated from cell line derived from spontaneous AKR tumour

Pedersen, Finn Skou; Crowther, R.; Tenney, Donald Y.; Reimold, Andreas M.; Haseltine, William A.

doi:10.1038/292167a0

Cited by 97 publications

(103 citation statements)

References 21 publications

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“…Two viruses designated GPA-V1 and GPA-V2 have been isolated in mouse SC-1 cells and identified by the molecular weight in NaDodSO4/ polyacrylamide gel electrophoresis and peptide maps of their pr-env proteins. GPA-V1 appears indistinguishable from the XC+, ecotropic virus (G-MuLV) that was isolated in 1965 by J. W. Hartley from Gross passage A virus extracts and which has been analyzed extensively (19,20,30,31). GPA-V2 is an XC-, dualtropic virus that appears related to AKR dualtropic (MCF) MuLV by the characteristic molecular weight and peptide map of its pr-env protein.…”

Section: Resultsmentioning

confidence: 99%

Leukemogenesis by Gross passage A murine leukemia virus: expression of viruses with recombinant env genes in transformed cells.

Famulari¹,

Koehne²,

O’Donnell³

1982

Proc. Natl. Acad. Sci. U.S.A.

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Gross passage A murine leukemia virus (MuLV) derived from extracts of C3Hf/Bi mouse leukemias has been shown to be a virus complex consisting of ecotropic, xenotropic, and recombinant, dualtropic MuLV components. The three virus components were distinguished biochemically by differences in the molecular weights and peptide maps oftheir primary env gene products synthesized in infected cells in vivo and in vitro. Virus expression was studied in primary leukemias induced in C3Hf/ Bi mice by Gross passage A virus extracts and by the individual ecotropic and recombinant MuLV components that were isolated in vitro. Our findings suggest that expression of the recombinant MuLV component of the Gross passage A virus complex is necessary and sufficient for the induction of leukemias in C3Hf/Bi mice. In contrast, induction of leukemias by the ecotropic virus component appears to involve generation of a second virus with characteristics of recombinant, dualtropic MuLV.

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Section: Resultsmentioning

confidence: 99%

Leukemogenesis by Gross passage A murine leukemia virus: expression of viruses with recombinant env genes in transformed cells.

Famulari¹,

Koehne²,

O’Donnell³

1982

Proc. Natl. Acad. Sci. U.S.A.

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“…XC + virus was rarely detected, but in one experiment an average of 5 plaques/dish (0.1 ml inoculum) was found. In contrast, simultaneous assays of Akv c and the previously cloned SL3-derived, fibroblast-adapted, XC ÷ viruses (SL3-1c, SL3-3~) (Pedersen et al, 1981) consistently revealed a high virus titre, 105 p.f.u./ml as measured by the XC assay. (This corresponds to an endpoint titre of 107 infectious units/ml.)…”

Section: Characterization Of Replication-competent Viruses Produced Bmentioning

confidence: 99%

“…The first attempts to clone the virus biologically from infected fibroblasts produced three isolates of 0022-1317/84/0000-6174 $02.00 © 1984 SGM oncogenic virus, one XC-and two XC ÷. All of these viruses had an ecotropic host range (Pedersen et al, 1981). The present report examines in depth the viruses produced by this cell line and their lymphomagenic properties.…”

Section: Introductionmentioning

confidence: 99%

“…These latter viruses are believed to represent recombinants between infectious ecotropic virus genomes and endogenous non-ecotropic virus-related sequences (Thomas & Coffin, 1982). Many, but not all, of these recombinant viruses are lymphomagenic (Hartley et al, 1977;Nowinski & Hays, 1978;Cloyd et al, 1980;Pedersen et al, 1981Pedersen et al, , 1982Hays et al, 1982). Lymphomagenesis in these systems is tested by inoculating newborn mice with the virus and observing either acceleration of the onset of thymic lymphoma in AKR mice, or induction of thymic lymphoma in other mouse strains.…”

Section: Introductionmentioning

confidence: 99%

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Several Classes of Retroviruses Are Produced by an AKR Mouse T Lymphoma Cell Line

Hays¹,

Swanson²,

Silva

1984

Journal of General Virology

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SUMMARYCharacterization of the viruses produced by the spontaneous T lymphoma cell line SL3 is presented. Using supernatant fluids or direct co-cultivation of cells, the SL3 cell line was found to produce replication-defective viruses in excess of replicationcompetent viruses. The replication-competent viruses released were predominantly those negative in the XC plaque assay (XC-); XC + viruses represented a minor population. However, when the SL3-derived viruses were passed in mouse embryo fibroblasts, XC-viruses were rarely recovered, and XC + viruses were readily isolated. These viruses were all ecotropic and lymphomagenic. Viruses with dual host range and nononcogenic ecotropic viruses were not isolated from the lymphoma cells. Two replication-defective viruses from SL3 cells were studied. Both could be rescued by non-oncogenic retroviruses and were then lymphomagenic. One defective virus appeared related to XC + viruses. In these studies, the XC ÷ and XC-viruses appeared to represent two different interference classes using separate cell receptors. Taken together, these experiments show that the SL3 T lymphoma cells replicate a variety of viruses most of which are lymphomagenic. Virus replication and/or virus integration may be the means of maintaining the malignant phenotype of these T lymphoma cells.

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“…103 Akv appears to have a weak enhancer composed of two copies of a 99-bp repeat sequence (Figure 4) that is preferentially expressed in B cells. 104 In a rare T cell lymphoma, the Akv provirus located near c-Myc had a partial duplication of the 99-bp enhancer element.…”

Section: How Does the Retroviral Ltr Influence The Type Of Disease Inmentioning

confidence: 99%

What retroviruses teach us about the involvement of c-Myc in leukemias and lymphomas

et al. 2002

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Novel leukaemogenic retroviruses isolated from cell line derived from spontaneous AKR tumour

Cited by 97 publications

References 21 publications

Leukemogenesis by Gross passage A murine leukemia virus: expression of viruses with recombinant env genes in transformed cells.

Leukemogenesis by Gross passage A murine leukemia virus: expression of viruses with recombinant env genes in transformed cells.

Several Classes of Retroviruses Are Produced by an AKR Mouse T Lymphoma Cell Line

What retroviruses teach us about the involvement of c-Myc in leukemias and lymphomas

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