Novel inhibition of cis/trans retinoic acid interconversion in biological fluids—an accurate method for determination of trans and 13-cis retinoic acid in biological fluids

Wang, Chao Jih; Pao, Li-Heng; Hsiong, Cheng Huei; Wu, Cheng-Shong; Whang‐Peng, Jacqueline; Hu, Oliver Yoa Pu

doi:10.1016/s1570-0232(03)00572-5

Cited by 17 publications

(10 citation statements)

References 18 publications

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“…Other metabolite functional groups may undergo E to Z isomerization (or vice versa) due to exposure to light or undesirable pH conditions to generate the parent drug. Such functional groups include O-methyloximes and carbon-carbon double bonds (Wang et al, 2003). Metabolites could also undergo epimerization to generate the drug (Testa et al, 1993;Won, 1994).…”

Section: Metabolite Interference Due To Conversion To the Parent Drugmentioning

confidence: 99%

Systematic LC‐MS/MS bioanalytical method development that incorporates plasma phospholipids risk avoidance, usage of incurred sample and well thought‐out chromatography

Jemal

Ouyang

Xia

2009

Biomedical Chromatography

181

124

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This treatise summarizes the underlying principle and the road map for systematic LC-MS/MS bioanalytical method development. The three themes that have recently emerged as central to building quality during method development-phospholipids, incurred sample and sound chromatographic considerations-are the main focus of this article. In order to reduce the bioanalytical risk associated with plasma phospholipids, a dual approach involving extraction and chromatography is recommended. The use of incurred sample during method development is essential to avoid interference arising from drug-related components. This is different from the current practice of incurred sample reanalysis, which tests reproducibility during method application. LC column/mobile phase optimization is needed to achieve appropriate peak shape, sensitivity and the separation of the analyte from interfering metabolites and phospholipids. Related to sound chromatographic considerations, we lay out facts and myths related to UPLC, vis-à-vis HPLC. Incorporation of quality during method development avoids the costly experience of finding out by chance about the invalidity of a method after it has been used in support of a number of pivotal clinical and non-clinical studies. To this end, we put forth an outline of a protocol for a systematic LC-MS/MS bioanalytical method development.

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Section: Metabolite Interference Due To Conversion To the Parent Drugmentioning

confidence: 99%

Systematic LC‐MS/MS bioanalytical method development that incorporates plasma phospholipids risk avoidance, usage of incurred sample and well thought‐out chromatography

Jemal

Ouyang

Xia

2009

Biomedical Chromatography

181

124

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“…Therefore, the quantitative determination of both forms is important and ex vivo conversion should be prevented. Several factors play a role in racemization and epimerization such as pH (oxazepam), light (SU5416), temperature (MK-0767), enzyme activity (retinoic acid), or the presence of thiol groups (retinoic acid) [44][45][46][47]. Stabilizing strategies are, therefore, similar to those mentioned previously.…”

Section: Prevention Of Intramolecular Conversionmentioning

confidence: 74%

Stabilization of Clinical Samples Collected for Quantitative Bioanalysis – A Reflection from The European Bioanalysis Forum

Hilhorst¹,

Amsterdam

Heinig

et al. 2015

Bioanalysis

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In bioanalysis of small molecules, the analyte concentration in the measured samples should reflect the concentration during sample collection. Precautions may be needed to prevent over- or under-estimation of the obtained result. This might require the addition of stabilizers to prevent degradation or nonspecific binding. For unstable drugs, it is essential to know how analytes can be stabilized before the start of the clinical study. Although the stabilization methods are well documented, the impact of the stabilization on the clinical workflow is not properly addressed. Already during method development, the clinical implications in terms of personnel safety, ease of use, training possibilities and staff capacity should be taken into account, and validation of the bioanalytical method should reflect collection procedures.

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“…Minimizing interconversion will depend on conditions during the bio-analytical procedure, pH being one of the most important factors, together with temperature. Other reasons for sample instability could be epimerization [120,121] or E to Z isomerisation reactions [122,123], influenced again by pH or light exposure.…”

Section: Stability Of Biological Samplesmentioning

confidence: 99%

A review of current trends and advances in modern bio-analytical methods: Chromatography and sample preparation

Nováková

Vlčková

2009

Analytica Chimica Acta

495

231

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Novel inhibition of cis/trans retinoic acid interconversion in biological fluids—an accurate method for determination of trans and 13-cis retinoic acid in biological fluids

Cited by 17 publications

References 18 publications

Systematic LC‐MS/MS bioanalytical method development that incorporates plasma phospholipids risk avoidance, usage of incurred sample and well thought‐out chromatography

Systematic LC‐MS/MS bioanalytical method development that incorporates plasma phospholipids risk avoidance, usage of incurred sample and well thought‐out chromatography

Stabilization of Clinical Samples Collected for Quantitative Bioanalysis – A Reflection from The European Bioanalysis Forum

A review of current trends and advances in modern bio-analytical methods: Chromatography and sample preparation

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