A series of sulfobetaine N‐chloramines, covalently combining an N‐chloramine unit and a sulfobetaine unit with varied intercharge linkers, were chemically synthesized. Preliminary antibacterial tests showed that the antibacterial activity decreased as the methylene linker increases from ‐(CH2)2‐ to ‐(CH2)8‐ except the linker of ‐(CH2)6‐, which exerted remarkable bactericidal efficacy unusually. In addition, the analogue with the linker of ‐(CH2)10‐ displayed the highest bactericidal efficacy among all synthetic N‐chloramines, probably due to its neutral zwitterionic characteristic on structure. Our research provided highly efficacious antibacterial agents together with the vital references to design novel ionic N‐chloramine biocides.