Novel histochemical approach for evaluation of tryptase expression in the mast cell population

Atiakshin, Dmitri; Герасимова, О. А.; Мешкова, В. Ю.; Самодурова, Н. Ю.; Самойленко, Т. В.; Шишкина, В. В.

doi:10.18499/2225-7357-2020-9-3-94-101

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…So, in May-Grünwald-Giemsa staining the cytoplasm of mast cells gains dark blue color, while the granules are red-stained [45]. The method reveals mucosal mast cells, and sometimes more effectively compared to toluidine blue, alcian blue, astra blue, or enzyme histochemistry or immunohistochemistry used (see below) [53,[65][66][67]. It should be noted that although May-Grünwald-Giemsa stain used to detect mast cells is highly selective, the procedure takes more time than toluidine blue staining [67].…”

Section: Reviewsmentioning

confidence: 99%

Modern Imaging Technologies of Mast Cells for Biology and Medicine (Review)

Grigor’ev¹,

Коржевский²

2021

Sovrem Tehnol Med

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Mast cells play an important role in the body defense against allergens, pathogens, and parasites by participating in inflammation development. However, there is evidence for their contributing to the pathogenesis of a number of atopic, autoimmune, as well as cardiovascular, oncologic, neurologic, and other diseases (allergy, asthma, eczema, rhinitis, anaphylaxis, mastocytosis, multiple sclerosis, rheumatoid arthritis, inflammatory gastrointestinal and pulmonary diseases, migraine, etc.). The diagnosis of many diseases and the study of mast cell functions in health and disease require their identification; so, the knowledge on adequate imaging techniques for mast cells in humans and different species of animals is of particular importance.The present review summarizes the data on major methods of mast cell imaging: enzyme histochemistry, immunohistochemistry, as well as histochemistry using histological stains. The main histological stains bind to heparin and other acidic mucopolysaccharides contained in mast cells and stain them metachromatically. Among these are toluidine blue, methylene blue (including that contained in May-Grünwald-Giemsa stain), thionin, pinacyanol, and others. Safranin and fluorescent dyes: berberine and avidin -also bind to heparin. Longer staining with histological dyes or alcian blue staining is needed to label mucosal and immature mast cells.Advanced techniques -enzyme histochemistry and especially immunohistochemistry -enable to detect mast cells high-selectively using a reaction to tryptases and chymases (specific proteases of these cells). In the immunohistochemical study of tryptases and chymases, species-specific differences in the distribution of the proteases in mast cells of humans and animals should be taken into account for their adequate detection. The immunohistochemical reaction to immunoglobulin E receptor (FcεRI) and c-kit receptor is not specific to mast cells, although the latter is important to demonstrate their proliferation in normal and malignant growth.Correct fixation of biological material is also discussed in the review as it is of great significance for histochemical and immunohistochemical mast cell detection.Fluorescent methods of immunohistochemistry and a multimarker analysis in combination with confocal microscopy are reported to be new technological approaches currently used to study various mast cell populations.

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Section: Reviewsmentioning

confidence: 99%

Modern Imaging Technologies of Mast Cells for Biology and Medicine (Review)

Grigor’ev¹,

Коржевский²

2021

Sovrem Tehnol Med

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Tryptase Profile of the Rat Skin Mast Cell Population During the Wound Healing

Шишкина

Klochkova

Alexeeva

et al. 2021

Žurnal anatomii i gistopatologii

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Mast cells cyclically synthesize and excrete a wide range of biogenesis products with different biological activities into the extracellular matrix and are regulators of local homeostasis both in normal conditions and in pathology – inflammation, oncogenesis, etc. The relative specificity of classical histochemical methods for detecting mast cells in relation to chromogenic to substrates causes certain difficulties in the selective study of the components of the secretome of mast cells, for example, heparin, histamine, chymase or tryptase. Therefore, immunomorphological techniques have become very popular, which identify specific substrates and allow differentiation of the components of the mast cell secretome. Mediators produced by mast cells promote neoangiogenesis, fibrillogenesis and re-epithelialization during the repair process.The aim of our work was to study the tryptase profile of the mast cell population of rat skin during the wound processusing an original combined method of immunohistochemical staining.Material and methods. The experiment involved 12 Wistar rats divided into two groups – intact (n=6) and with the existing wound process of the skin in the withers (n=6). The tryptase profile of mast cells was assessed on the 7th day of the wound process in comparison with the control group.Results. The results obtained showed a significant increase in the number of tryptase-positive mast cells on the 7th day of the wound process in the skin against the background of a general increase in the population of mast cells. Intragranular tryptase reserve was significantly increased. In contrast to the control, where mast cells with single tryptase-positive granules dominated, during the wound process, cells of this type were practically not detected in the skin (43.69±2.9% and 8.55±0.9%). The content of tryptase-positive mast cells with complete filling of the cytoplasm in the control group and the group of animals with a wound process was 14.24±1.2% and 38.03±2.9%, respectively.Conclusion. Thus, when modeling a wound, an increase in tryptase synthesis is detected both in individual MCs and within the entire MC population. This fact indicates that mast cell proteases can become a potential therapeutic target for improving wound regeneration by correcting immunogenesis, inflammation and fiber formation.

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Novel histochemical approach for evaluation of tryptase expression in the mast cell population

Cited by 2 publications

References 15 publications

Modern Imaging Technologies of Mast Cells for Biology and Medicine (Review)

Modern Imaging Technologies of Mast Cells for Biology and Medicine (Review)

Tryptase Profile of the Rat Skin Mast Cell Population During the Wound Healing

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