Novel Gene Regulation in Normal and Abnormal Spermatogenesis

Du, Lijun; Chen, Wei; Cheng, Zhiguang; He, Jian; Han, Lu; He, Zuping; Qin, Weibing

doi:10.3390/cells10030666

Cited by 28 publications

(10 citation statements)

References 146 publications

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“…Spermatogenesis is a complex process involving proliferation, differentiation and apoptosis. 54 Activation of the P53 signaling pathway has been shown to induce apoptosis in testicular spermatogenic cells in the presence of high levels of oxidative stress. 55,56 In the current study, we found individual exposure to noise and CO caused apoptosis of germ cells.…”

Section: Discussionmentioning

confidence: 99%

Effects and possible mechanisms of combined exposure to noise and carbon monoxide on male reproductive system in rats

Zhang

Wang

et al. 2023

Environmental Toxicology

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Environmental hazards are an increasing concern due to the rapid pace of industrialization. Among these hazards, noise and carbon monoxide (CO) are common risk factors and have been shown to cause serious health problems. However, existing studies focused on the individual effects of noise and CO exposure and the combined effects of these two factors remain poorly understood. Our study aimed to examine the combined effects of noise and CO exposure on testicular function by constructing individual and combined exposure models. Our findings indicated that combined exposure to noise and CO was associated with a higher risk of testicular damage and male reproductive damage when compared to exposure alone. This was evidenced by poorer semen quality and more severe pathological damage to the testis. This combined exposure led to higher levels of oxidative stress and apoptosis in the testes, with bioinformatics analyses suggesting the signaling pathways involved in these responses. Specifically, activation of the P53 signaling pathway was found to contribute to the testicular damage caused by the combined exposure. Encouragingly, pterostilbene (PTE), a novel phytochemical, alleviated combined exposure‐induced testicular damage by reducing oxidative stress and germ cell apoptosis. Overall, we identified joint reproductive toxicity resulting from the exposure to noise and CO, and found that PTE is a promising potential treatment for injuries caused by these factors.

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Section: Discussionmentioning

confidence: 99%

Effects and possible mechanisms of combined exposure to noise and carbon monoxide on male reproductive system in rats

Zhang

Wang

et al. 2023

Environmental Toxicology

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“…42,55 Spermatogenesis is a key process in male reproduction, involving many cellular (somatic cell populations, Leydig, Sertoli, and peritubular myoid cells) and molecular (gonadotropins, testosterone, and estrogen) players, which are essential factors in a process that culminates on differentiation of the germinative epithelium to mature sperm cells. 56,57 This complex process can be severely impaired by external conditions, including WD intake and its resulting comorbidities. [11][12][13][14] In general, our WD protocol…”

Section: Discussionmentioning

confidence: 99%

A mixture of glyphosate and 2,4‐D herbicides enhances the deleterious reproductive outcomes induced by Western diet in obese male mice

Valente,

de Matos Manoel,

Reis

et al. 2023

Environmental Toxicology

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The consumption of Western diet (WD) – enriched in fats and sugars – is associated with overweight, obesity and male reproductive disorders. In addition to WD intake, crops and dairy products display residues of herbicides, including glyphosate and 2,4‐D that are widely applied worldwide. The concomitant exposure to WD and herbicides – mimicking contemporary scenarios – is not fully investigated. Thus, we evaluated the effects of glyphosate and 2,4‐D, alone or in mixture, on WD‐induced alterations in the male genital system. Male C57BL6J mice were submitted to WD (chow containing 20% lard, 0.2% cholesterol, 20% sucrose, and high sugar solution with 23.1 and 18.9 g/L of D‐fructose and D‐glucose) for 6 months. Concomitantly to WD, the animals received glyphosate (0.05, 5, or 50 mg/kg/day), 2,4‐D (0.02, 2 or 20 mg/kg/day) or their mixture (0, 05 + 0.02, 5 + 2, or 50 + 20 mg/kg/day) by intragastrical administration (5×/week). Doses were based on Acceptable Daily Intake (ADIs) or No Observed Adverse Effect Level (NOAEL) values. Herbicide exposure did not alter the WD‐induced obesity, hypercholesterolemia and hyperglycemia. WD induced sperm cell abnormalities, reduced the number, volume and area of Leydig cells, enhanced the frequency of epididymal abnormalities, decreased the proliferation in both germinal and epididymal epithelia, and reduced the number of androgen receptor (AR) positive epididymal cells. Remarkably, the herbicide mixtures promoted such WD‐induced effects: increased the frequency of sperm cell and epididymal abnormalities (absence of sperm, cytoplasmic vacuoles, and clear cell hypertrophy) (5 + 2 and 50 + 20 doses); decreased Leydig cell nuclei volume and area (5 + 2 and 50 + 20 doses), reduced epididymal cell proliferation (all mixtures), and AR expression (50 + 20 dose). In addition, herbicide mixtures reduced serum testosterone levels (5 + 2 and 50 + 20 doses). Our findings indicate that the mixture of glyphosate and 2,4‐D herbicides, mimicking environmentally relevant scenarios, promotes WD‐induced changes in the male genital system.

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“…DNA and histone methylation contribute to regulate gene expression throughout all spermatogenesis (Carrell, 2019; Du et al, 2021; Rajender et al, 2011). Interestingly, different aspects of lifestyle such as diet, toxicant exposure, air pollution, smoking, or work conditions affect the pattern of DNA and histone methylation, in human and mouse models, which may impact in the etiology of sperm pathologies such as globozoospermia (Comhaire et al, 2017; Dong et al, 2017; Lu et al, 2019).…”

Section: The Round‐headed Spermatozoamentioning

confidence: 99%

Human globozoospermia‐related genes and their role in acrosome biogenesis

Moreno

2022

WIREs Mechanisms of Disease

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The mammalian acrosome is a secretory vesicle attached to the sperm nucleus whose fusion with the overlying plasma membrane is required to achieve fertilization. Acrosome biogenesis starts during meiosis, but it lasts through the entire process of haploid cell differentiation (spermiogenesis). Acrosome biogenesis is a stepwise process that involves membrane traffic from the Golgi apparatus, but it also seems that the lysosome/endosome system participates in this process. Defective sperm head morphology is accompanied by defective acrosome shape and function, and patients with these characteristics are infertile or subfertile. The most extreme case of acrosome biogenesis failure is globozoospermia syndrome, which is primarily characterized by the presence of round‐headed spermatozoa without acrosomes with cytoskeleton defects around the nucleus and infertility. Several genes participating in acrosome biogenesis have been uncovered using genetic deletions in mice, but only a few of them have been found to be deleted or modified in patients with globozoospermia. Understanding acrosome biogenesis is crucial to uncovering the molecular basis of male infertility and developing new diagnostic tools and assisted reproductive technologies that may help infertile patients through more effective treatment techniques. This article is categorized under: Reproductive System Diseases > Environmental Factors Infectious Diseases > Stem Cells and Development Reproductive System Diseases > Molecular and Cellular Physiology

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Novel Gene Regulation in Normal and Abnormal Spermatogenesis

Cited by 28 publications

References 146 publications

Effects and possible mechanisms of combined exposure to noise and carbon monoxide on male reproductive system in rats

Effects and possible mechanisms of combined exposure to noise and carbon monoxide on male reproductive system in rats

A mixture of glyphosate and 2,4‐D herbicides enhances the deleterious reproductive outcomes induced by Western diet in obese male mice

Human globozoospermia‐related genes and their role in acrosome biogenesis

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