Novel focused OCT-LIF endoscope

Abstract

Combined optical coherence tomography (OCT) and laser-induced fluorescence (LIF) endoscopy has shown higher sensitivity and specificity for distinguishing normal tissue from adenoma when compared to either modality alone. Endoscope optical design is complicated by the large wavelength difference between the two systems. A new high-resolution endoscope 2 mm in diameter is presented that can create focused beams from the ultraviolet to near-infrared. A reflective design ball lens operates achromatically over a large wavelength range, and employs TIR at two faces and reflection at a third internal mirrored face. The 1:1 imaging system obtains theoretically diffraction-limited spots for both the OCT (1300 nm) and LIF (325 nm) channels.

Coregistered autofluorescence-optical coherence tomography imaging of human lung sections

“…[8][9][10] When used in combination AF-OCT imaging systems can provide biochemical information colocalized with structural information. [17][18][19][20][21][22][23] Double-clad fiber-based endoscopic dual-modality systems have been reported with common paths for the two modalities. One approach was to have the two separate imaging systems combined together either in free space using dichroic mirrors [11][12][13][14][15][16] or by using an endoscopic probe with separate adjacent fibers for the two modalities.…”

Animal Biotechnology III

“…Moreover, combined techniques (ultrasound, laser, infra-red camera, forceps; Hunter et al, 2000;Wall et al, 2011), documentation (videos, pictures;Nezhat, 1994), and handling (disinfection etc.) of the endoscopes for different purposes have made this technique indispensable not only in the medical field (Wall et al, 2011).…”

Gabor domain optical coherence microscopy combined with laser scanning confocal fluorescence microscopy

“…Fluorescence spectra of tumors and healthy tissues from uterine cervixes and vulvas were compared with OCT structural visualization [11], and this attempt first cast light upon the complementary relationship between the two imaging techniques: their combination has the potential to decrease the false-positivity of fluorescence detection and improve the specificity of OCT-based diagnosis. Simultaneous but parallel operation of OCT and fluorescence imaging was achieved by attaching a fluoroscope to an OCT endoscope [12][13][14][15]. In a study of early bladder cancer in rats, a multimodal endoscopic OCT system improved the sensitivity and the specificity of 5-ALA fluorescence detection by 21% and 28%, respectively [16].…”