Coregistered autofluorescence-optical coherence tomography imaging of human lung sections

Pahlevaninezhad, Hamid; Lee, Anthony; Lam, Stephen; MacAulay, Calum; Lane, Pierre

doi:10.1117/1.jbo.19.3.036022

Cited by 17 publications

(10 citation statements)

References 48 publications

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“…The strong AF signal on the right of the AF map due to the cartilage band just under the luminal surface confirms that cartilage produces larger autofluorescence signal. This is consistent with the results on ex vivo human airway sections presented in [43].…”

Section: Resultssupporting

confidence: 82%

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A high-efficiency fiber-based imaging system for co-registered autofluorescence and optical coherence tomography

Pahlevaninezhad¹,

Lee²,

Shaipanich³

et al. 2014

Biomed. Opt. Express

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Abstract:We present a power-efficient fiber-based imaging system capable of co-registered autofluorescence imaging and optical coherence tomography (AF/OCT). The system employs a custom fiber optic rotary joint (FORJ) with an embedded dichroic mirror to efficiently combine the OCT and AF pathways. This three-port wavelength multiplexing FORJ setup has a throughput of more than 83% for collected AF emission, significantly more efficient compared to previously reported fiber-based methods. A custom 900 µm diameter catheter -consisting of a rotating lens assembly, double-clad fiber (DCF), and torque cable in a stationary plastic tube -was fabricated to allow AF/OCT imaging of small airways in vivo. We demonstrate the performance of this system ex vivo in resected porcine airway specimens and in vivo in human on fingers, in the oral cavity, and in peripheral airways. 36. S. Liang, A. Saidi, J. Jing, G. Liu, J. Li, J. Zhang, C. Sun, J. Narula, and Z. Chen, "Intravascular atherosclerotic imaging with combined fluorescence and optical coherence tomography probe based on a double-clad fiber combiner," J.

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Section: Resultssupporting

confidence: 82%

“…Higher AF signals correspond to the areas with higher fluorophore concentrations. Cartilage and dense connective tissue include high concentrations of highly fluorescing collagen and elastin fibers [43]. Therefore, we expect that the regions with strong AF signal include cartilage and dense connective tissue.…”

Section: Resultsmentioning

confidence: 99%

A high-efficiency fiber-based imaging system for co-registered autofluorescence and optical coherence tomography

Pahlevaninezhad¹,

Lee²,

Shaipanich³

et al. 2014

Biomed. Opt. Express

Self Cite

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show abstract

“…Since then, some authors have demonstrated the effectiveness of the OCT in combination with AFB to distinguish invasive cancer from CIS and dysplasia from metaplasia, hyperplasia, or normal tissue, using the quantitative measurement of the epithelial thickness (38,39). Furthermore Pahlevaninezhad et al recently reported the combination of Doppler OCT with AFI in 31 patients to guide and improve biopsy of pulmonary nodules, resulting a powerful tool to evaluate pulmonary parenchyma as well (40).…”

Section: Oct Is a Non-invasive Optical Imaging Technique Based Onmentioning

confidence: 99%

The role of bronchoscopy in the diagnosis of early lung cancer: a review

Andolfi¹,

Potenza²,

Capozzi³

et al. 2016

J. Thorac. Dis.

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Lung cancer is the leading cause of cancer-related deaths worldwide with an overall 5-year survival rate of 17% after diagnoses. Indeed many patients tend to have a very poor prognosis, due to being diagnosed at an advanced stage. Conversely patients who are diagnosed at an early stage have a 5-year survival >70%, indicating that early detection of lung cancer is crucial to improve survival. Although flexible bronchoscopy is a relatively non-invasive procedure for patients suspected of having lung cancer, only 29% of carcinoma in situ (CIS) and 69% of microinvasive tumors were detectable using white light bronchoscopy (WLB) alone. As a result, in the past two decades, new bronchoscopic techniques have been developed to increase the yield and diagnostic accuracy, such as autofluorescence bronchoscopy (AFB), narrow band imaging (NBI) and high magnification bronchovideoscopy (HMB). However, due to the low specificity and the limitation to detect only proximal bronchial tree, new probe-based technologies have been introduced: radial endobronchial ultrasound (R-EBUS), optical coherence tomography (OCT), confocal laser endomicroscopy (CLE) and laser Raman spectroscopy (LRS). To date, although tissue biopsy remains the gold standard for diagnosing malignant/premalignant airway disease and some techniques are still investigational, bronchoscopic technologies can be considered the safest and most accurate tools to evaluate both central and distal airway mucosa.

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“…Combinations of OCT and fluorescence imaging techniques have been developed and applied to various tissues such as the coronary artery [50,51], colon [52][53][54][55], bladder [56], lung [57] and oral cavity [11,58]. Other multimodal approaches for oral cavity detection include combinations of FLIM and reflectance confocal microscopy (RCM) [59], white light imaging and autofluorescence imaging [60], FLIM, UBM, and photoacoustic imaging (PAI) [61], and OCT and RCM [62].…”

Section: Introductionmentioning

confidence: 99%

In vivo wide-field reflectance/fluorescence imaging and polarization-sensitive optical coherence tomography of human oral cavity with a forward-viewing probe

Yoon

Jang

Xiao

et al. 2015

Biomed. Opt. Express

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Abstract:We report multimodal imaging of human oral cavity in vivo based on simultaneous wide-field reflectance/fluorescence imaging and polarization-sensitive optical coherence tomography (PS-OCT) with a forward-viewing imaging probe. Wide-field reflectance/fluorescence imaging and PS-OCT were to provide both morphological and fluorescence information on the surface, and structural and birefringent information below the surface respectively. The forward-viewing probe was designed to access the oral cavity through the mouth with dimensions of approximately 10 mm in diameter and 180 mm in length. The probe had field of view (FOV) of approximately 5.5 mm in diameter, and adjustable depth of field (DOF) from 2 mm to 10 mm by controlling numerical aperture (NA) in the detection path. This adjustable DOF was to accommodate both requirements for image-based guiding with high DOF and high-resolution, high-sensitivity imaging with low DOF. This multimodal imaging system was characterized by using a tissue phantom and a mouse model in vivo, and was applied to human oral cavity. Information of surface morphology and vasculature, and under-surface layered structure and birefringence of the oral cavity tissues was obtained. These results showed feasibility of this multimodal imaging system as a tool for studying oral cavity lesions in clinical applications. 281-284 (1996). 6. M. Olivo, R. Bhuvaneswari, and I. Keogh, "Advances in bio-optical imaging for the diagnosis of early oral cancer," Pharmaceutics 3(4), 354-378 (2011). 7 fold imaging with polarization sensitive optical coherence tomography and a MEMS scanning catheter," Opt. ©2015 Optical Society of America

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Coregistered autofluorescence-optical coherence tomography imaging of human lung sections

Cited by 17 publications

References 48 publications

A high-efficiency fiber-based imaging system for co-registered autofluorescence and optical coherence tomography

A high-efficiency fiber-based imaging system for co-registered autofluorescence and optical coherence tomography

The role of bronchoscopy in the diagnosis of early lung cancer: a review

In vivo wide-field reflectance/fluorescence imaging and polarization-sensitive optical coherence tomography of human oral cavity with a forward-viewing probe

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