Novel fluorescence labeling reagent 4-(carbazole-9-yl)-benzyl chloroformate and its application in the determination of nitrofuran metabolites compounds in foodstuffs by high performance liquid chromatography with fluorescence detection

Yu, Yanxin; Li, Ning; Jin, Qianqian; Ji, Zhongyin; Sun, Zhi‐Wei; Li, Guoliang; Zhang, Shijuan; You, Jing

doi:10.1016/j.microc.2018.10.021

Cited by 20 publications

(11 citation statements)

References 27 publications

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“…The requirement of temperature for derivatization in different literature reports was referenced for better comparison. 15 , 18 , 44 Excessive temperature may cause decomposition of the product, so 37, 50, and 60 °C were selected as the screening temperatures for ultrasound-assisted derivatization. To avoid erroneous conclusions about the interaction between the derivatization temperature and time, we tested different temperatures and simultaneously screened the derivatization time.…”

Section: Resultsmentioning

confidence: 99%

“… 13 , 14 At present, the minimum performance limit (MRPL) of these residual compounds in meat products stipulated by the EU is 1.0 μg/kg. 2 , 15 In this context, it is essential to develop a fast and sensitive detection method for NF metabolite compounds for solving food safety problems.…”

Section: Introductionmentioning

confidence: 99%

“… 18 In general, acid hydrolysis and derivatization reactions are carried out in a 37 °C water bath for 16 h, but it is impossible to get results quickly. 15 , 19 , 20 Compared with water bath derivatization, ultrasonic-assisted derivatization has a better relative response ratio, which can significantly shorten the reaction time and reduce the complexity of the target compound analysis. 21 , 22 Ultrasonic energy can accelerate the derivatization reaction because ultrasonic energy can increase the number of effective collisions between the reactants and make the derivatization reaction more efficient.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

et al. 2020

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Nitrofuran (NF) is a class of broad-spectrum antibiotics that are used illegally in animal feeding. NF and its metabolites have proven to pose potential risk to human health. To address the current analytical needs to quantify low levels of NF metabolites in animal foods, a sensitive method was developed for simultaneous detection of four NF metabolites in fish products by an ultraperformance liquid chromatography-diode array detector (UPLC-DAD). With 2-nitrobenzaldehyde (2-NBA) as the derivatizing reagent, the metabolites were hydrolyzed and derivatized under the assistance of thermostatic ultrasound. Compared with the current detection methods, the time of the derivatization reaction has been shortened from 16 to 2 h. The relative coefficient of four NF metabolite derivatives reached more than 0.998, with excellent linear relationship. The limits of detection (LODs) and limits of quantification (LOQs) of six repeated determinations reached 0.25–0.33 and 0.80–1.10 μg/kg, respectively. For all four NF metabolites, the limit of detection of the method was below the minimum required performance limit (MRPL) of 1.0 μg/kg, which makes it compatible with the EU requirements. The recoveries ranging from 89.8 to 101.9% with relative standard deviation below 6.5% were obtained for all of the NF metabolites. What’s more, this method was successfully applied for the determination of four NF metabolites in the fish products. As a promising approach, this method could also be extended for the quantitation of NF metabolites in aquaculture and poultry products.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

et al. 2020

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“…Presently available detection methods for antibiotics include mass spectrometry, high performance liquid chromatography, LC with MS detection, electrochemical analysis and so on . Most of these approaches are expensive apparatus‐dependant, operation‐complicated and time‐consuming.…”

Section: Introductionmentioning

confidence: 99%

A Highly Stable (4, 8)‐Connected Tb‐MOF Exhibiting Efficiently Luminescent Sensing towards Nitroimidazole Antibiotics

Yang

Wang

et al. 2019

Zeitschrift anorg allge chemie

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A robust 4,8-connected Tb-based metal-organic framework (Tb-MOF) with paddle wheel-shaped {Tb 2 (COO) 4 } subunits extended by C 3 -symmetric 3,3Ј,3ЈЈ-[1,3,5-benzenetriyltris(carbonylimino)]trisbenzoate connector was hydrothermally synthesized, showing highly environmental stability, good dispersion and intense green emission in water system. Resulting critically from the well suppressed absorption towards the excitation energy, the Tb-MOF exhibits rapid and efficient 23 fluorescent response towards nitroimidazole antibiotics with strong quenching constants and low detection limits of 1.59 ϫ 10 4 m -1 and 2.4 μM for metronidazole as well as 1.62 ϫ 10 4 m -1 and 2.9 μM for dimetridazole. Moreover, the sensitive and selective identification of the Tb-MOF has strong anti-interference and excellent regeneration ability, which endows the promising applications of the Tb-MOF as fluorescent sensing materials.

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“…The use of o‐phthalaldehyde in connection with thiol‐containing compounds (e.g. 3‐mercaptopropionic acid, 2‐mercaptoethanol) has been widely applied to the determination of low concentrations of GABA analogs via FLD analysis . However, thiol‐containing derivatives are unstable.…”

Section: Introductionmentioning

confidence: 99%

HPLC determination of γ‐aminobutyric acid and its analogs in human serum using precolumn fluorescence labeling with 4‐(carbazole‐9‐yl)‐benzyl chloroformate

You

Sun

et al. 2019

J of Separation Science

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In this study, a simple analytical method for the determination of γ‐aminobutyric acid, gabapentin, and baclofen by using high‐performance liquid chromatography with fluorescence detection was developed. An amidogen‐reactive fluorescence labeling reagent, 4‐(carbazole‐9‐yl)‐benzyl chloroformate was first used to sensitively label these analytes. The completed labeling of these analytes can be finished rapidly only within 5 min at the room temperature (25°C) to form 4‐(carbazole‐9‐yl)‐benzyl chloroformate labeled fluorescence derivatives. These labeled derivatives expressed strong fluorescence property with the maximum excitation and emission wavelengths of 280 and 380 nm, respectively. The labeled derivatives were analyzed using a reversed‐phase Eclipse SB‐C18 column within 10 min with satisfactory shapes. Excellent linearity (R2 > 0.995) for all analytes was achieved with the limits of detection and the limits of quantitation in the range of 0.25−0.35 and 0.70−1.10 μg/L, respectively. The proposed method was used for the simultaneous determination of γ‐aminobutyric acid and its analogs in human serum with satisfactory recoveries in the range of 94.5–97.5%.

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Novel fluorescence labeling reagent 4-(carbazole-9-yl)-benzyl chloroformate and its application in the determination of nitrofuran metabolites compounds in foodstuffs by high performance liquid chromatography with fluorescence detection

Cited by 20 publications

References 27 publications

Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

Determination of Nitrofuran Metabolites in Fish by Ultraperformance Liquid Chromatography-Photodiode Array Detection with Thermostatic Ultrasound-Assisted Derivatization

A Highly Stable (4, 8)‐Connected Tb‐MOF Exhibiting Efficiently Luminescent Sensing towards Nitroimidazole Antibiotics

HPLC determination of γ‐aminobutyric acid and its analogs in human serum using precolumn fluorescence labeling with 4‐(carbazole‐9‐yl)‐benzyl chloroformate

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