Novel electrochemiluminescent assay for the aptamer-based detection of testosterone

Are aptamer-based biosensing approaches a good choice for female fertility monitoring? A comprehensive review

“…For example, aptamers are < USD 50/g compared with ~USD 300/g for recombinant monoclonal antibodies produced using mammalian cell lines (Hammerschmidt et al, 2014;Izzi-Engbeaya et al, 2020;Walter et al, 2012). Besides, high temperatures, harsh pH conditions, or drastically high levels of salt would irreversibly denature antibody proteins, while an RNA or DNA aptamer would merely unfold temporarily (see Section 3) (Cánovas et al, 2022;Chen and Yang, 2015).…”

“…Aptamers are short ssDNA or RNA oligonucleotides first described in 1990, consisting typically of 40-100 nucleotides, and proposed as a promising alternative to commercially available antibodies (Cánovas et al, 2022). At optimized conditions, aptamers form a unique threedimensional structure due to intramolecular interactions such as hydrogen bonding, electrostatic interactions, hydrophobic interactions, and Van der Waals forces between the nucleotides.…”

Interpol Review of Drug Analysis 2019-2022

“…sulfonamides, gentamycin, and cephalexin), antibacterial compounds (Macrolide, beta-Lactam. Chloramphenicol, Sulfur drugs, and Gentamicin), organochlorine pesticides, dichlorodiphenyldichloroethylene, dichlorodiphenyltrichloroethane, alachlor, and organophosphate in meat products [ 743 ]; LC-UV and LC-MS/MS methods for the determination of selected steroid hormones [ 744 ]; LC-APPI-MS/MS method for simultaneous determination of endogenous steroids (11-deoxycortisol, 11-ketotestosterone, 17 alpha and 178-estradiol, 17 alpha-hydroxyprogesterone, 17,208-dihydroxyprogesterone, 17,208,21-trihydroxyprogesterone, androstenedione, cortisol, estriol, estrone, progesterone, and testosterone) [ 745 ]; MS method for characterization of anabolic-androgenic steroids in illegal seized samples [ 746 ]; GC coupled to isotope ratio MS (GC-C-IRMS) method for analysis of 19-norandrosterone and 19-noretiocholanolone [ 747 ]; sensing platform for detection of testosterone [ 748 ]; mu ATR-FTIR mapping for forensic analysis of the composition of anabolic steroid tablet [ 749 ]; colorimetric aptasensor for the detection of testosterone [ 750 ]; CE and UHPLC for determining steroids in water [ 751 ]; MALDI-MS method for detection of steroids [ 752 ]; 2022 electrochemical probe for trace determination of the Steroid 11-Desoxycorticosterone [ 753 ]; aptamer-based assays coupled with electrochemiluminescence sensing for detection of testosterone [ 754 ]; LC-HRMSMS for the analysis of ABS TRACT Dehydrochloromethyltestosterone (DHCMT) [ 755 ]; HPLC-UV detection of designer steroid 17 beta-hydroxy 5 alpha-androst-1-en-3-one cypionate in an injectable liquid and subsequently characterized using HRAM-MS, NMR spectrometry, and GC-MS [ 756 ]; origami paper-based analytical device based on ELISA for testosterone detection [ 757 ]; GC-MS/MS method for simultaneous detection of 93 anabolic steroids in dietary supplements [ 758 ].…”

Recent Progress on Highly Selective and Sensitive Electrochemical Aptamer-based Sensors

“…Lin et al [84] reported a sensor, which hybridizes nucleic acid by heating electrode itself rather than heating solution system. The aptamers are hybridized with the complementary chains on the electrode De Wael et al [163] used the change of the overall configuration of the aptamer after binding the target to control the hybridization between the end of the nucleic acid chain and the complementary chain modified on the electrode, and then detected testosterone by ECL method. Han et al [164] reported a new complex sensor by using nanoparticles and double ring structure on the electrode surface through the combination of Based on the hybridization ability of nucleic acids, amplification strategies can be designed.…”