Novel electrochemical sensor based on polydopamine molecularly imprinted polymer for sensitive and selective detection of Acinetobacter baumannii

Roushani, Mahmoud; Sarabaegi, Masoumeh; Rostamzad, Arman

doi:10.1007/s13738-020-01936-9

Cited by 27 publications

(11 citation statements)

References 24 publications

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“…The use of the zot gene as an internal amplification control both for the PCR amplifications and amperometric readings in this study, eliminates the possibility of false negatives that could arise due to the presence of inhibitors in the sample. Other previously reported studies [ 41 , 42 , 43 , 45 ] that developed different biosensors for A. baumannii detection did not include any internal controls.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, it would be efficient for use in tropical environments as well. Other studies that reported similar electrochemical biosensors for A. baumannii detection [ 41 , 42 , 43 , 45 ] did not perform any stability evaluations to determine the performance of the method under varied temperatures.…”

Section: Discussionmentioning

confidence: 99%

“…The electrochemical signals generated by the reduction of β-CD were recorded using differential pulse voltammetry (DPV) [ 42 ]. A more recent effort by Roushani and co-workers [ 43 ] showed the detection of A. baumannii from human serum samples using a molecularly imprinted polymer (MIP) on a glassy carbon electrode with electropolymerization of a dopamine monomer and A. baumannii template.…”

Section: Introductionmentioning

confidence: 99%

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Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

et al. 2022

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Acinetobacter baumannii (A. baumannii) are phenotypically indistinguishable from the Acinetobacter calcoaceticus–A. baumannii (ACB) complex members using routine laboratory methods. Early diagnosis plays an important role in controlling A. baumannii infections and this could be assisted by the development of a rapid, yet sensitive diagnostic test. In this study, we developed an enzyme-based electrochemical genosensor for asymmetric PCR (aPCR) amplicon detection of the blaOXA-51-like gene in A. baumannii. A. baumannii blaOXA-51-like gene PCR primers were designed, having the reverse primer modified at the 5′ end with FAM. A blaOXA-51-like gene sequence-specific biotin labelled capture probe was designed and immobilized using a synthetic oligomer (FAM-labelled) deposited on the working electrode of a streptavidin-modified, screen-printed carbon electrode (SPCE). The zot gene was used as an internal control with biotin and FAM labelled as forward and reverse primers, respectively. The blaOXA-51-like gene was amplified using asymmetric PCR (aPCR) to generate single-stranded amplicons that were detected using the designed SPCE. The amperometric current response was detected with a peroxidase-conjugated, anti-fluorescein antibody. The assay was tested using reference and clinical A. baumannii strains and other nosocomial bacteria. The analytical sensitivity of the assay at the genomic level and bacterial cell level was 0.5 pg/mL (1.443 µA) and 103 CFU/mL, respectively. The assay was 100% specific and sensitive for A. baumannii. Based on accelerated stability performance, the developed genosensor was stable for 1.6 years when stored at 4 °C and up to 28 days at >25 °C. The developed electrochemical genosensor is specific and sensitive and could be useful for rapid, accurate diagnosis of A. baumannii infections even in temperate regions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

et al. 2022

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“…Compared to the other receptors including antibodies, DNA and aptamers, MIP has several unique superiorities, such as cost-effective, high stability and simple preparation [16][17][18]. Over the past decades, MIP has been confirmed to show great application values in the detection of protein, virus, and many organic molecules [19][20][21]. In recent years, there are several MIP-like sensors developed for pathogen detection [22,23], this inspired us that it may be a perfect proposal to design a MIP-like electrochemical sensor for LM detection.…”

Section: Introductionmentioning

confidence: 99%

Pathogen-Imprinted Polymer Film Integrated probe/Ti3C2Tx MXenes Electrochemical Sensor for Highly Sensitive Determination of Listeria Monocytogenes

Jiang

Ding

et al. 2022

J. Electrochem. Sci. Technol

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As one of the most hazardous and deadliest pathogens, Listeria monocytogenes (LM) posed various serious diseases to the human being, thus designing effective strategy for its detection is of great significance. In this work, by preparing Ti 3 C 2 T x MXenes nanoribbon (Ti 3 C 2 T x R) as carrier and selecting thionine (Th) acted simultaneously as signal probe and functional monomer, a LM pathogen-imprinted polymers (PIP) integrated probe electrochemical sensor was design to monitor LM for the first time, that was carried out through the electropolymerization of Th on the Ti 3 C 2 T x R/GCE surface in the existence of LM. Upon eluting the templates from the LM imprinted cavities, the fabricated PIP/Ti 3 C 2 T x R/GCE sensor can rebound LM cells effectively. By recording the peak current of Th as the response signal, it can be weakened when LM cell was re-bound to the LM imprinted cavity on PIP/Ti 3 C 2 T x R/GCE, and the absolute values of peak current change increase with the increasement of LM concentrations. After optimizing three key parameters, a considerable low analytical limit (2 CFU mL -1 ) and wide linearity (10-10 8 CFU mL -1 ) for LM were achieved. In addition, the experiments demonstrated that the PIP/Ti 3 C 2 T x R sensor offers satisfactory selectivity, reproducibility and stability.

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“…Moreover, advances on transducers, for example carbon based nanostructured materials, enable achieving outstanding performances of MIPs [ 12 ]. From our perspective, with past and present active research on polymer thin film preparation, surface interactions and applications in sensor science, we wished to address the crucial point of three kinds of toxic pollutants: organics represented by pesticides (for example, the glyphosate case [ 13 ]), metal ions owing to their growing occurrence in water and soils [ 14 ], and finally bacteria due to the sanitary issues they raise [ 15 ] as well as the increasing demand for food security [ 16 ] on the one hand, and the ever-lasting problem of nosocomial diseases, on the other hand [ 17 ]. The three types of pollutants differ in composition and size, and require completely different imprinting techniques as will be discussed at length in Section 5 .…”

Section: Introductionmentioning

confidence: 99%

Towards Clean and Safe Water: A Review on the Emerging Role of Imprinted Polymer-Based Electrochemical Sensors

Zheng

Khaoulani

Ktari³

et al. 2021

Sensors

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This review critically summarizes the knowledge of imprinted polymer-based electrochemical sensors for the detection of pesticides, metal ions and waterborne pathogenic bacteria, focusing on the last five years. MIP-based electrochemical sensors exhibit low limits of detection (LOD), high selectivity, high sensitivity and low cost. We put the emphasis on the design of imprinted polymers and their composites and coatings by radical polymerization, oxidative polymerization of conjugated monomers or sol-gel chemistry. Whilst most imprinted polymers are used in conjunction with differential pulse or square wave voltammetry for sensing organics and metal ions, electrochemical impedance spectroscopy (EIS) appears as the chief technique for detecting bacteria or their corresponding proteins. Interestingly, bacteria could also be probed via their quorum sensing signaling molecules or flagella proteins. If much has been developed in the past decade with glassy carbon or gold electrodes, it is clear that carbon paste electrodes of imprinted polymers are more and more investigated due to their versatility. Shortlisted case studies were critically reviewed and discussed; clearly, a plethora of tricky strategies of designing selective electrochemical sensors are offered to “Imprinters”. We anticipate that this review will be of interest to experts and newcomers in the field who are paying time and effort combining electrochemical sensors with MIP technology.

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Novel electrochemical sensor based on polydopamine molecularly imprinted polymer for sensitive and selective detection of Acinetobacter baumannii

Cited by 27 publications

References 24 publications

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

Pathogen-Imprinted Polymer Film Integrated probe/Ti3C2Tx MXenes Electrochemical Sensor for Highly Sensitive Determination of Listeria Monocytogenes

Towards Clean and Safe Water: A Review on the Emerging Role of Imprinted Polymer-Based Electrochemical Sensors

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