Novel determination of a new antiviral combination; sofosbuvir and velpatasvir by high performance thin layer chromatographic method; application to real human samples

Rezk, Mamdouh R.; Monir, Hany H.; Marzouk, Hoda M.

doi:10.1016/j.microc.2019.02.012

Cited by 36 publications

(19 citation statements)

References 6 publications

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“…In this study, the green mobile phase was obtained by the simple mixture of ethanol and water (green solvents) in comparison to the normal HPTLC method. The application of RP-HPTLC methods offer several advantages over normal phase HPTLC methods [ 37 , 40 ]. In addition, green RP-HPTLC methods for the simultaneous determination of 6-SHO and 6-GIN also reduce the toxicity of the proposed method to the environment [ 35 , 36 ].…”

Section: Resultsmentioning

confidence: 99%

“…Some HPTLC methods have also been reported for the determination of bioactive compounds, such as vanillin and flavonoids in plant extracts, but they used toxic mobile phase in addition to different analyzed compounds [ 34 , 35 ]. Recently, the analytical methods associated with green analytical chemistry or environmentally benign analytical chemistry have increased significantly in literature [ 31 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

“…Based on reported literature, it was found that green HPTLC methods have not been reported for the analysis of 6-SHO or 6-GIN alone or in their combination. Moreover, green reversed phase HPTLC (RP-HPTLC) methods offer many advantages over conventional HPTLC methods, such as the avoidance of nonpolar fractions, avoidance of interference of the impurities, formation of compact spots, detection clarity, and non-toxicity to the environment [ 36 , 37 , 38 ]. Hence, the aim of this study was to develop and validate a green RP-HPTLC method for the simultaneous determination of 6-SHO and 6-GIN in traditional and ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas for the first time.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Determination of 6-Shogaol and 6-Gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-Densitometry Method

Foudah

Shakeel

Yusufoğlu

et al. 2020

Foods

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Various analytical methodologies have been reported for the determination of 6-shogaol (6-SHO) and 6-gingerol (6-GIN) in ginger extracts and commercial formulations. However, green analytical methods for the determination of 6-SHO and 6-GIN, either alone or in combination, have not yet been reported in literature. Hence, the present study was aimed to develop a rapid, simple, and cheaper green reversed phase high-performance thin-layer chromatography (RP-HPTLC) densitometry method for the simultaneous determination of 6-SHO and 6-GIN in the traditional and ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas. The simultaneous analysis of 6-SHO and 6-GIN was carried out via RP-18 silica gel 60 F254S HPTLC plates. The mixture of green solvents, i.e., ethanol:water (6.5:3.5 v/v) was utilized as a mobile phase for the simultaneous analysis of 6-SHO and 6-GIN. The analysis of 6-SHO and 6-GIN was performed at λmax = 200 nm for 6-SHO and 6-GIN. The densitograms of 6-SHO and 6-GIN from traditional and ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas were verified by obtaining their single band at Rf = 0.36 ± 0.01 for 6-SHO and Rf = 0.53 ± 0.01 for 6-GIN, compared to standard 6-SHO and 6-GIN. The green RP-HPTLC method was found to be linear, in the range of 100–700 ng/band with R2 = 0.9988 for 6-SHO and 50–600 ng/band with R2 = 0.9995 for 6-GIN. In addition, the method was recorded as “accurate, precise, robust and sensitive” for the simultaneous quantification of 6-SHO and 6-GIN in traditional and ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas. The amount of 6-SHO in traditional extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas was obtained as 12.1, 17.9, 10.5, and 9.6 mg/g of extract, respectively. However, the amount of 6-SHO in ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas were obtained as 14.6, 19.7, 11.6, and 10.7 mg/g of extract, respectively. The amount of 6-GIN in traditional extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas were found as 10.2, 15.1, 7.3, and 6.9 mg/g of extract, respectively. However, the amount of 6-GIN in ultrasonication-assisted extracts of ginger rhizome, commercial ginger powder, commercial capsules, and commercial ginger teas were obtained as 12.7, 17.8, 8.8, and 7.9 mg/g of extract, respectively. Overall, the results of this study indicated that the proposed analytical technique could be effectively used for the simultaneous quantification of 6-SHO and 6-GIN in a wide range of plant extracts and commercial formulations.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Determination of 6-Shogaol and 6-Gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-Densitometry Method

Foudah

Shakeel

Yusufoğlu

et al. 2020

Foods

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“…37 Eco-friendly RP-HPTLC method for the analysis of rivaroxaban will reduce the environmental toxicity caused by routine toxic solvents used in routine pharmaceutical analysis. 33,34 Our research work gave a very good analytical technique for the estimation of the rivaroxaban in NPs formulation.…”

Section: Methods Developmentmentioning

confidence: 97%

“…[29][30][31] Green HPTLC technique offers several advantages over routine HPTLC in the analysis of drugs or pharmaceutical compounds. [32][33][34] Interestingly, no eco-friendly analytical methodology had been applied yet for the analysis of rivaroxaban in pharmaceutical formulations or biological uids. Moreover, no reports on the determination of rivaroxaban biomarker in nanoparticles (NPs) formulation utilizing the RP-HPLC technique are available in the literature so far.…”

Section: Introductionmentioning

confidence: 99%

Ecofriendly densitometric RP-HPTLC method for determination of rivaroxaban in nanoparticle formulations using green solvents

et al. 2020

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Thin‐layer chromatography/fluorescence detection approach for sensitive and selective determination of hepatitis C virus antiviral (velpatasvir): application to human plasma

et al. 2020

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A validated thin-layer chromatography (TLC) method combined with fluorescence detection mode was developed for the selective determination of a recently approved anti-hepatitis C virus (HCV) drug (velpatasvir). The separation was performed on silica gel 60 F 254 plates using ethylacetate:methanol:triethylamine (48:1.5:1.0, v/v/v) as a mobile phase. Plates were scanned in the fluorescence mode after excitation at 335 nm. This method provided an excellent separation of velpatasvir from sofosbuvir with R F values of 0.22 and 0.46 for velpatasvir and sofosbuvir, respectively, after scanning the developed plates in the ultraviolet detection mode at 335 nm. The calibration curve was linear over the range 4-40 ng/band with a correlation coefficient of 0.9994. The developed procedure was validated according to ICH guidelines with a detection limit of 1.30 ng/band and quantitation limit of 3.95 ng/band. The suggested method could selectively determine velpatasvir with high sensitivity in a synthetic tablet powder containing a co-formulated anti-HCV drug (sofosbuvir) without any interference from excipients or sofosbuvir. In addition, the method was successfully applied for determination of velpatasvir in spiked human plasma with adequate % recovery.

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Novel determination of a new antiviral combination; sofosbuvir and velpatasvir by high performance thin layer chromatographic method; application to real human samples

Cited by 36 publications

References 6 publications

Simultaneous Determination of 6-Shogaol and 6-Gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-Densitometry Method

Simultaneous Determination of 6-Shogaol and 6-Gingerol in Various Ginger (Zingiber officinale Roscoe) Extracts and Commercial Formulations Using a Green RP-HPTLC-Densitometry Method

Ecofriendly densitometric RP-HPTLC method for determination of rivaroxaban in nanoparticle formulations using green solvents

Thin‐layer chromatography/fluorescence detection approach for sensitive and selective determination of hepatitis C virus antiviral (velpatasvir): application to human plasma

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