Novel cyanotoxin-producing Synechococcus in tropical lakes

Gin, Karina Yew-Hoong; Sim, Zhi Yang; Goh, Kwan Chien; Kok, Jerome Wai Kit; Te, Shu Harn; Trần, Ngọc Hân; Li, Wenxuan; He, Yiliang

doi:10.1016/j.watres.2021.116828

Cited by 22 publications

(22 citation statements)

References 62 publications

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“…Indeed, phylogenetic trees based on host cyanobacterial 16S sequences showed that Synechococcus strain SR-R4S1 and strain SR-C6 displayed some genetic proximity to marine Synechococcus spp. ( 47 ). However, there is insufficient evidence to show their marine origin, and further study is needed to evaluate this possibility.…”

Section: Resultsmentioning

confidence: 99%

“…Upon isolation, the isolates were then incubated and maintained in batch culture at 25°C under low irradiance (20 μmol photons m −2 s −1 ) with a 12-h/12-h light/dark cycle. Identification of the strains was determined through whole-genome sequencing ( 47 ). The phylogenetic tree based on 16S rRNA sequence revealed that SR-C6 and SR-R4S1 are present in a mixed-cluster where freshwater and marine Synechococcus strains coexist ( 47 ).…”

Section: Methodsmentioning

confidence: 99%

“…Identification of the strains was determined through whole-genome sequencing ( 47 ). The phylogenetic tree based on 16S rRNA sequence revealed that SR-C6 and SR-R4S1 are present in a mixed-cluster where freshwater and marine Synechococcus strains coexist ( 47 ).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Novel Freshwater Cyanophages Provide New Insights into Evolutionary Relationships between Freshwater and Marine Cyanophages

Zhang

Gin

2021

Microbiol Spectr

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Novel Freshwater Cyanophages Provide New Insights into Evolutionary Relationships between Freshwater and Marine Cyanophages

Zhang

Gin

2021

Microbiol Spectr

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“…strain SR-C1 was isolated as described previously (Zhang et al, 2020). The strains were isolated by micro-pipetting from a freshwater lake sample into sterile MLA medium (Bolch and Blackburn, 1996) at 25 • C. Identification of the strains was determined through whole genome sequencing (Gin et al, 2021). The cultures were then incubated and maintained in batch culture at 25 • C under low irradiance (20 µmol photons m −2 s −1 ) with a 12-h/12-h light/dark cycle.…”

Section: Host Cellsmentioning

confidence: 99%

“…Strain SR-C1. Both the host and phage were isolated from a tropical urban freshwater ( Gin et al, 2021 ). Unlike the previous discovery of S-SRP01 and S-CRM01, S-SRP02 does not cluster with any established phylogenetic groups of cyanoviruses infecting Synechococcus or Prochlorococcus .…”

Section: Introductionmentioning

confidence: 99%

Genomic Characterization of a Novel Freshwater Cyanophage Reveals a New Lineage of Cyanopodovirus

Zhang

Gin

2022

Front. Microbiol.

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Cyanobacteria are one of the dominant autotrophs in tropical freshwater communities, yet phages infecting them remain poorly characterized. Here we present the characterization of cyanophage S-SRP02, isolated from a tropical freshwater lake in Singapore, which infects Synechococcus sp. Strain SR-C1 isolated from the same lake. S-SRP02 represents a new evolutionary lineage of cyanophage. Out of 47 open reading frames (ORFs), only 20 ORFs share homology with genes encoding proteins of known function. There is lack of auxiliary metabolic genes which was commonly found as core genes in marine cyanopodoviruses. S-SRP02 also harbors unique structural genes highly divergent from other cultured phages. Phylogenetic analysis and viral proteomic tree further demonstrate the divergence of S-SRP02 from other sequenced phage isolates. Nonetheless, S-SRP02 shares synteny with phage genes of uncultured phages obtained from the Mediterranean Sea deep chlorophyll maximum fosmids, indicating the ecological importance of S-SRP02 and its related viruses. This is further supported by metagenomic mapping of environmental viral metagenomic reads onto the S-SRP02 genome.

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Microbial‐Enabled Photosynthetic Oxygenation for Disease Treatment

Yang,

Li,

Feng

et al. 2023

Adv Funct Materials

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Oxygen as one of the most critical substances in living organisms has attracted ever‐increasing attention in disease treatment, which is important in regulating metabolic activities. However, the hypoxia arising from disease is a prevalent problem, leading to observably reduced therapeutic effectiveness in photodynamic therapy, radiotherapy, sonodynamic therapy, etc. Therefore, the reversion of hypoxia becomes the basis for enhancing the disease treatment. Thanks to the development of nanotechnology, various nanomaterials with oxygen‐production capacity are explored to recover the function of oxygen in tissue, but there are still some limitations. The photosynthetic microorganisms (PSMs) are extensively applied for improving hypoxia in diseases due to their highly efficient photocatalytic oxygen production efficacy and desirable biocompatibility. In this review, the up‐to‐date research progress on therapeutic modalities of microbial‐based photosynthetic oxygenation (e.g., cancer treatment, wound healing, and tissue engineering) are summarized and highlighted. In addition, the key issue of biocompatibility/biosafety of microbial‐based treatment that is fundamental to apply in vivo is further emphasized and clarified. Finally, the present critical issues are discussed and the future evolution of microbial‐based treatment based on photosynthetic oxygenation is predicted, promoting further development and clinical applications.

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Novel cyanotoxin-producing Synechococcus in tropical lakes

Cited by 22 publications

References 62 publications

Novel Freshwater Cyanophages Provide New Insights into Evolutionary Relationships between Freshwater and Marine Cyanophages

Novel Freshwater Cyanophages Provide New Insights into Evolutionary Relationships between Freshwater and Marine Cyanophages

Genomic Characterization of a Novel Freshwater Cyanophage Reveals a New Lineage of Cyanopodovirus

Microbial‐Enabled Photosynthetic Oxygenation for Disease Treatment

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