Novel Freshwater Cyanophages Provide New Insights into Evolutionary Relationships between Freshwater and Marine Cyanophages

Zhang, Dong; He, Yiliang; Gin, Karina Yew‐Hoong

doi:10.1128/spectrum.00593-21

Cited by 12 publications

(3 citation statements)

References 70 publications

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“…Furthermore, the Pelagibacter siphovirus Kolga [ 100 ] and more than 20 autographiviruses showed significant sequence identity over a DNA primase, ribonucleotide diphosphate reductase subunits, and a few small hypothetical proteins ( Figure S13 ). Moreover, two Synechococcus siphoviruses, KBS-S-2A [ 101 ] and S-CBS4 [ 102 ], were similar to Synechococcus autographiviruses S-SRP02 [ 103 ], S-B28 (NC_048171.1), and S-SRP01 [ 104 ] through a few hypothetical proteins ( Figure S14 ). It has not escaped our attention that the above-mentioned phages of the SG3 subgraph infect similar hosts, that is, marine bacteria of the Alphaproteobacteria class (often of the same taxonomic genus).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Three Novel Virulent Aeromonas Phages Provides Insights into the Diversity of the Autographiviridae Family

Bujak

Decewicz

Kitowicz

et al. 2022

Viruses

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In this study, we isolated and characterized three novel virulent Autographiviridae bacteriophages, vB_AspA_Bolek, vB_AspA_Lolek, and vB_AspA_Tola, which infect different Aeromonas strains. These three host–pathogen pairs were derived from the same sampling location—the arsenic-containing microbial mats of the Zloty Stok gold mine. Functional analysis showed they are psychrotolerant (4–25 °C), albeit with a much wider temperature range of propagation for the hosts (≤37 °C). Comparative genomic analyses revealed a high nucleotide and amino acid sequence similarity of vB_AspA_Bolek and vB_AspA_Lolek, with significant differences exclusively in the C-terminal region of their tail fibres, which might explain their host range discrimination. The protein-based phage network, together with a phylogenetic analysis of the marker proteins, allowed us to assign vB_AspA_Bolek and vB_AspA_Lolek to the Beijerinckvirinae and vB_AspA_Tola to the Colwellvirinae subfamilies, but as three novel species, due to their low nucleotide sequence coverage and identity with other known phage genomes. Global comparative analysis showed that the studied phages are also markedly different from most of the 24 Aeromonas autographiviruses known so far. Finally, this study provides in-depth insight into the diversity of the Autographiviridae phages and reveals genomic similarities between selected groups of this family as well as between autographiviruses and their relatives of other Caudoviricetes families.

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Section: Resultsmentioning

confidence: 99%

Characterization of Three Novel Virulent Aeromonas Phages Provides Insights into the Diversity of the Autographiviridae Family

Bujak

Decewicz

Kitowicz

et al. 2022

Viruses

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“…Cyanophage research has concentrated on saline and freshwater aquatic environments [131]. A notable study has indicated genetic similarities between cyanophages in marine and freshwater systems [135]. Cyanophages such as Ma-LMM01, Ma-LBP, and VLP are known to specifically attack cyanobacteria that produce the toxin microcystin [136].…”

Section: Viruses For Habs Controlmentioning

confidence: 99%

Advancements in Biological Strategies for Controlling Harmful Algal Blooms (HABs)

Anabtawi,

Lee,

Al-Anazi

et al. 2024

Water

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Harmful algal blooms (HABs) are a primary environmental concern, threatening freshwater ecosystems and public health and causing economic damages in the billions of dollars annually. These blooms, predominantly driven by phytoplankton species like cyanobacteria, thrive in nutrient-rich, warm, and low-wind environments. Because of the adverse impacts of HABs, this review examines various control methods, focusing on biological strategies as sustainable solutions. While effective in disrupting algal populations, traditional chemical and physical interventions carry ecological risks and can be resource-intensive. Biological control methods, including biomanipulation and using algicidal microorganisms such as Streptococcus thermophiles, Myxobacteria, and Lopharia spadicea, emerge as eco-friendly alternatives offering long-term benefits. Additionally, barley and rice straw application has demonstrated efficacy in curbing HAB growth. These biological approaches work by inhibiting algal proliferation, disrupting cellular structures, and fostering algal cell aggregation. Despite their advantages over conventional methods, biological controls face challenges, including intricate ecological interactions. This article delves into the latest biological techniques aimed at eradicating HABs, intending to diminish their frequency and reduce toxin levels in aquatic environments. While most research to date has been confined to laboratory settings, scaling these methods to field applications presents hurdles due to the variability and complexity of natural ecosystems. The review underscores the need for further research and development in this critical area of environmental science.

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“…The cyanophages, which specifically infect cyanobacteria, also possess very narrow host ranges. They are involved in regulating the abundance and population density of cyanobacteria, thus playing a key role in cyanobacterial community succession 8 . A-1(L) is a Myoviridae cyanophage isolated in 1970s 9 that specifically infects the model cyanobacterium Anabaena sp.…”

Section: Introductionmentioning

confidence: 99%

Structure of the intact tail machine of Anabaena myophage A-1(L)

Yu,

Yang,

Zhang

et al. 2024

Nat Commun

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The Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution cryo-EM structure of its intact tail machine including the neck, tail and attached fibers. Besides the dodecameric portal, the neck contains a canonical hexamer connected to a unique pentadecamer that anchors five extended bead-chain-like neck fibers. The 1045-Å-long contractile tail is composed of a helical bundle of tape measure proteins surrounded by a layer of tube proteins and a layer of sheath proteins, ended with a five-component baseplate. The six long and six short tail fibers are folded back pairwise, each with one end anchoring to the baseplate and the distal end pointing to the capsid. Structural analysis combined with biochemical assays further enable us to identify the dual hydrolytic activities of the baseplate hub, in addition to two host receptor binding domains in the tail fibers. Moreover, the structure of the intact A-1(L) also helps us to reannotate its genome. These findings will facilitate the application of A-1(L) as a chassis cyanophage in synthetic biology.

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Novel Freshwater Cyanophages Provide New Insights into Evolutionary Relationships between Freshwater and Marine Cyanophages

Abstract: This study expands the current knowledge on freshwater cyanophage isolates and cyanophage genetic diversity, indicating that freshwater and marine cyanophages infecting Synechococcus spp. may share close genetic similarity and evolutionary relationships.

Cited by 12 publications

References 70 publications

Characterization of Three Novel Virulent Aeromonas Phages Provides Insights into the Diversity of the Autographiviridae Family

Characterization of Three Novel Virulent Aeromonas Phages Provides Insights into the Diversity of the Autographiviridae Family

Advancements in Biological Strategies for Controlling Harmful Algal Blooms (HABs)

Structure of the intact tail machine of Anabaena myophage A-1(L)

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