Novel Crabtree negative yeast from rumen fluids can improve rumen fermentation and milk quality

Abstract

Upgrading the nutritive value of rice straw (RS) is necessary to increase its contribution to enhancing meat and milk production. Present work verified whether novel Crabtree negative yeast inoculant could promote RS utilization, rumen fermentation, and milk quality in tropical crossbred lactating Holstein cows. The new stain of Crabtree negative yeasts (Pichia kudriavzevii KKU20 and Candida tropicalis KKU20) was isolated from the rumen of dairy cattle. This study used 6 multiparous crossbreds between Holstein Frisian × Zebu dairy cows in their mid-lactation period. Dairy cows were randomly allocated to three ensiled RS with various yeast stains including Saccharomyces cerevisiae, P. kudriavzevii KKU20, and C. tropicalis KKU20 according to a 3 × 3 replicated Latin square design. Crabtree-negative yeast (P. kudriavzevii and C. tropicalis) increased the apparent digestibility of dry matter by about 6.9% when compared with Crabtree-positive yeast (S. cerevisiae). Bacterial populations were highest with ensiled RS by C. tropicalis KKU20. Ensiled RS with Crabtree-negative yeasts were significantly increased with total volatile fatty acids, but they did not affect volatile fatty acid profiles. Milk protein precentage was highest at 35.6 g/kg when C. tropicalis was fed, and lowest when applied with S. cerevisiae and P. kudriavzevii KKU20 in ensiled RS at 34.5 and 34.1 g/kg, respectively. Thus, feeding ensiled RS with novel Crabtree negative yeast could improve RS digestion, rumen fermentation, and milk protein content in dairy cows.

The Effect of Yeast and Roughage Concentrate Ratio on Ruminal pH and Protozoal Population in Thai Native Beef Cattle

“…Dietary composition has the greatest influence on volatile fatty acid content; manipulating the diet by increasing the level of concentrate diet is a strategy to improve ruminal fermentation by increasing propionogenesis [ 45 ]. Such a typical propionic acid fermentation reaction was found in the current investigation, and as a result, the C 2 :C 3 ratio decreased, which is consistent with prior findings [ 4 , 46 , 47 , 48 ]. This increase in C 3 concentration can be attributed to increased nonfiber carbohydrate consumption.…”

Effect of enzyme and probiotic supplementation on growth performance, nutrient digestibility, carcass traits, and meat quality of Simmental steers

“…In addition, yeast can competitively bind to the binding sites in the gastrointestinal tract with the toxins produced by pathogenic bacteria and help to eliminate the toxins, which is advantageous to enhance the growth performance of cattle (Ma et al, 2021). It has also been found that the addition of brewer's yeast to cattle diets can improve the microecological environment of the rumen, regulate the pH in the rumen, increase feed conversion, and thus improve the performance of cattle (Suntara et al, 2021). Meanwhile, cellulase was also more commonly used in beef cattle production, and had a good promotion effect on the performance of beef cattle (Dehghan-Banadaky et al, 2013).…”

Impact of Probiotics on Dairy Production Efficiency

“…In addition, researchers have also recorded the qualitative improvement in milk production using probiotic interventions. A study conducted on lactating Holstein cows by Suntara et al (2021b) presented an interesting fact that administration of animal feed prepared with Crabtree-negative yeast ( P. kudriavzevii KKU20 and C. tropicalis KKU20) increased the milk protein content. The reason was connected to the increased beneficial microbial population in the rumen, which enhanced the amount of microbial crude protein.…”