Novel Cr (III), Fe (III) and Ru (III) Vanillin Based Metallo‐Pharmaceuticals for Cancer and Inflammation Treatment: Experimental and Theoretical Studies

Abstract

In this study, three novel complexes comprising trivalent Cr (III), Fe (III) and Ru (III) with imine ligand derived from 2-amino-3-hydroxypyridine and ovanillin (H 2 L) have been synthesized and characterized via wide range of spectroscopic and analytical tools such as 1 H NMR and 13 C NMR, infrared (IR) and UV-Vis spectrophotometry, conductivity and magnetic measurements. The obtained results along with DFT data confirmed a 1:1 (metal: ligand) stoichiometry with non-planner geometries for the three complexes. The binding action and the docking study of the prepared metal-complexes to calf thymus DNA was also studied by absorption spectra and viscosity technique, which revealed that the three complexes interact strongly with DNA through intercalative binding mode. Significantly, these metal-imine complexes showed strong and efficient anti-inflammatory and antimicrobial activities against various gram-positive (Microccus luteus), gram-negative (Escherichia coli and Serratia marcescence) bacteria, and three strains of fungus. Moreover, all complexes exhibited more potent cytotoxicity effect on the outgrowth of different types of carcinoma cells, including human colon (HCT-116 cell line), breast (MCF-7 cell line), and hepatic cellular (HepG-2), than the clinically-proven Vinblastine standard.

Non-Linear Optical Property and Biological Assays of Therapeutic Potentials Under In Vitro Conditions of Pd(II), Ag(I) and Cu(II) Complexes of 5-Diethyl amino-2-({2-[(2-hydroxy-Benzylidene)-amino]-phenylimino}-methyl)-phenol

“…For the 1:1 complex, the stability constant (K f ) was calculated from spectrophotometric measurements according to the relation presented in our previous work [18,19].…”

“…From the stability constants, the change of free energy ∆(G ) was calculated at 25 • C using the relation presented in previous studies [19][20][21].…”

“…The antimicrobial activity of the new ligand and its metal complexes was screened against strains of Gram (positive) bacteria such as Staphylococcus aureus and Bacillus subtilis, and Gram (negative) bacteria, such as Escherichia coli as well as fungus, namely Candida albicans, Aspergillus flavus and Trichophyton Rubrumin, using the agar well dilution method [19,[35][36][37]. The synthesized ligand and its complexes, CuL, PdL and AgL, were dissolved with concentrations of 10 and 20 mg mL −1 in dimethyl sulfoxide (DMSO).…”

“…For comparison, the gentamycin drug (standard antibacterial) and fluconazole (standard antifungal) were tested under the same conditions. [19,38,39]. The binding constants (K b ) of the new complexes, have been determined utilizing the spectroscopic titration data applying the following Equation (1):…”

Synthesis, DFT calculation, DNA-binding, antimicrobial, cytotoxic and molecular docking studies on new complexes VO(II), Fe(III), Co(II), Ni(II) and Cu(II) of pyridine Schiff base ligand

“…Shimadzu TG-60H thermal analyzers were used to follow the thermal degradation of the compounds. Magnetic susceptibility measurements were conducted using a Gouy balance [16,17]. Mass spectra were recorded using DI analysis Shimadzu Qp-2010 plus.…”