Novel Composite Matrices Modified with Nanolayers of Polymers as Perspective Materials for Separation of Biomolecules and Bioanalysis

Abstract

The effectiveness of the developed adsorbents used for DNA isolation and purification was confirmed in comparison with the standard methods. Thus, a facile (alternative in relation to irradiation postpolymerization or standard oxidative polymerization techniques) manufacturing method of the materials for nucleic acids sample preparation was developed.

New Composite Materials Modified with Nano-Layers of Functionalized Polymers for Bioanalysis and Medical Diagnostics

“…Last phenomenon was not studied in details until now. Indeed, Prostyakova, Kapustin and Zubov (2011) have demonstrated, that after ozone treatment of silica samples followed by removing of unabsorbed ozone and other gaseous products, iodometric titration confirms the formation of peroxide species. The amount of those species is negligible in "pure" silica materials but increases for the samples with considerable amount of admixtures (presumably iron oxides).…”

“…However, the techniques originally used for synthesis of PTFE-and PANI-modified composite adsorbents despite a certain similarity of their chromatographic properties were quite different, complicated and difficult for scaling up. In the case of PTFE-containing material it was γ-ray induced postpolymerization of tetrafluoroethylene (TFE) in adsorption layers at low temperatures (Kapustin et al, 2006). In the case of PANI-containing material it was oxidative precipitation polymerization of aniline in aqueous phase followed by deposition of PANI nano-particles onto the carrier surface.…”

“…H o w e v e r , t h e u s e o f P A N I -c o n t a i n i n g a d s o r b e n t s o f f e r s a n i m p r e s s i v e a d v a n t a g e i n separation of mixtures of proteins, since it does not require the addition of an organic component to the mobile phase. A typical example of the pH-gradient separation of a model protein mixture is given in (Kapustin et al, 2006). The proteins eluted from the column in the order of ascending pI values of the individual components.…”

“…The development of biocompatible composites modified with fluoropolymer-and polyaniline-based modifiers is in the scope of this work. Both the polytetrafluoroethylene (PTFE) and the polyaniline (PANI) were shown to be effective for one-step DNA isolation from the complex mixtures containing DNA, RNA and proteins (as well as low-molecular substances) (Kapustin et al, 2003(Kapustin et al, , 2006. A number of different methods were described, which relates to the synthesis, characterization of the properties and practical use of the adsorbents coated with PTFE and PANI.…”

“…However, original techniques of manufacture of such materials did not find a wide application due to technological difficulties connected with their synthesis or purification. For example, in a case of PTFE the difficulty is determined by need of irradiation source, in a case of PANI the main complication is concerned with formation of insoluble polymer particles, which are not retained with the adsorbent surface, and therefore this overlaborates the procedure of a final product washing out (Kapustin et al, 2006). Usually manufacturing of the chromatographic composite adsorbents is based on a number of consequtive physical-chemical transformations.…”