Novel Coatings to Minimize Bacterial Adhesion and Promote Osteoblast Activity for Titanium Implants

Camargo, Samira Esteves Afonso; Roy, Tanaya; Carey, Patrick H.; Fares, Chaker; Ren, F.; Clark, A. E.; Esquivel‐Upshaw, Josephine F.

doi:10.3390/jfb11020042

Cited by 22 publications

(16 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although complete prevention of bacterial adhesion on biomaterials is complicated, various efforts have been developed to effectively minimize and control the bacterial adhesion on biomaterial surfaces [ 15 , 20 , 33 , 34 ]. In this way, new coatings have been developed on the surface of titanium to decrease the biofilm [ 6 , 7 , 15 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium

et al. 2021

Self Cite

View full text Add to dashboard Cite

The objective of this study was to investigate the potential of titanium nanotubes to promote the proliferation of human osteoblasts and to reduce monomicrobial biofilm adhesion. A secondary objective was to determine the effect of silicon carbide (SiC) on these nanostructured surfaces. Anodized titanium sheets with 100–150 nm nanotubes were either coated or not coated with SiC. After 24 h of osteoblast cultivation on the samples, cells were observed on all titanium sheets by SEM. In addition, the cytotoxicity was evaluated by CellTiter-BlueCell assay after 1, 3, and 7 days. The samples were also cultivated in culture medium with microorganisms incubated anaerobically with respective predominant periodontal bacteria viz. Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia as monoinfection at 37 °C for 30 days. The biofilm adhesion and coverage were evaluated through surface observation using Scanning Electron Microscopy (SEM). The results demonstrate that Ti nanostructured surfaces induced more cell proliferation after seven days. All groups presented no cytotoxic effects on human osteoblasts. In addition, SEM images illustrate that Ti nanostructured surfaces exhibited lower biofilm coverage compared to the reference samples. These results indicate that Ti nanotubes promoted osteoblasts proliferation and induced cell proliferation on the surface, compared with the controls. Ti nanotubes also reduced biofilm adhesion on titanium implant surfaces.

show abstract

Section: Discussionmentioning

confidence: 99%

Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although complete prevention of bacterial adhesion on biomaterials is complicated, various efforts, such as fabricating thin anti-bacterial films or coatings, have been developed to effectively minimize and control the bacterial adhesion biomaterial surface [ 27 , 28 ]. Camargo et al [ 29 ] demonstrated that coatings based on SiC and TiN applied on Ti surfaces were effective against P. gingivalis in vitro.…”

Section: Discussionmentioning

confidence: 99%

Novel Coatings to Minimize Corrosion of Titanium in Oral Biofilm

et al. 2021

Self Cite

View full text Add to dashboard Cite

The aim of this work is to investigate the effects produced by polymicrobial biofilm (Porphyromonas gingivalis, Streptococcus mutans, Streptococcus sanguinis, and Streptococcus salivarius) on the corrosion behavior of titanium dental implants. Pure titanium disks were polished and coated with titanium nitride (TiN) and silicon carbide (SiC) along with their quarternized versions. Next, the disks were cultivated in culture medium (BHI) with P. gingivalis, S. mutans, S. sanguinis, and S. salivarius and incubated anaerobically at 37 °C for 30 days. Titanium corrosion was evaluated through surface observation using Scanning Electron Microscope (SEM) and Atomic Force Microscopy (AFM). Furthermore, the Ti release in the medium was evaluated by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). SEM images showed that coated Ti disks exhibited lower corrosion compared to non-coated disks, except for the quartenized TiN. This was confirmed by AFM, where the roughness was higher in non-coated Ti disks. ICP showed that Ti levels were low in all coating disks. These results indicate that these SiC and TiN-based coatings could be a useful tool to reduce surface corrosion on titanium implant surfaces.

show abstract

“…As the surface roughness reduces from the sub-micron scale to the nano-scale level, two phenomena occur -the surface state gradually changes from hydrophobic to hydrophilic and becomes increasingly unfavorable for the adhesion of most bacteria. Second, the anchoring points for bacterial adhesion gradually decrease, weakening the bacterial-surface bonding strength [77,78]. At the same time, the nanometric roughness ensures integrin activation leading to higher osteoblast attachment [79].…”

Section: Dimensional Analysis Of Ch/hamentioning

confidence: 99%

“…The physicochemical properties of an implant surface, including hydrophobicity, surface free energy and roughness are an absolute necessity for selective osteoblast adhesion [77]. As seen in Fig.…”

Section: Osteoblast Adhesion/ Proliferation Studies On Ch/hamentioning

confidence: 99%

Nano-hydroxyapatite coating synthesized on quasi-fibrillar superstructures of collagen hydrolysate leads to superior osteoblast proliferation when compared to nano-hydroxyapatite synthesized on collagen fibrils

Banerjee

Bajaj

Bhat

et al. 2021

Preprint

View full text Add to dashboard Cite

This study had a two-fold objective: To utilize collagen hydrolysate for synthesizing a nanoscale Hydroxyapatite (HA) coating that would act as a superior osteoblast adhesion/ proliferation agent compared to collagen-derived HA (C/HA) and to comprehend the significant role played by structural constraints on HA nucleation. Collagen was extracted from pacu skin with a high yield of 65.3% (w/w of tissue). It was digested by collagenase and the hydrolysate (CH) was purified with a high yield of 0.68g/g of collagen. The CH peptides had a mass of 6kDa, a predominant PP-II conformation and formed self-assembling hierarchical structures at physiological pH with an average dimension of 842nm. The HA synthesized on CH (CH/HA) displayed higher yield when compared to C/HA. Structural analysis of CH/HA revealed that the PP-II peptides coiled to form mimic-helical moieties with reduced intermolecular packing distance of 0.9nm. The mimic helices cross-linked to form a vast quasi-fibrillar network that was comparatively smaller than collagen fibrils but exhibited enhanced stability and greater dynamicity. CH/HA displayed intense calcium-carboxyl interactions, sharper diffraction planes, smaller size of 48.6nm and a Ca/P ratio closer to 1.69 when compared to C/HA along with displaying serrated edge blooming crystals. Because of the small size, the CH/HA nanocrystals displayed significantly better osteoblast adhesion than C/HA and reduced the doubling time of cells. Overall, the results indicated that CH based nanocomposites displayed suitable morphological characteristics and cellular response for potential application as implant and bone graft coating material.

show abstract

Novel Coatings to Minimize Bacterial Adhesion and Promote Osteoblast Activity for Titanium Implants

Cited by 22 publications

References 33 publications

Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium

Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium

Novel Coatings to Minimize Corrosion of Titanium in Oral Biofilm

Nano-hydroxyapatite coating synthesized on quasi-fibrillar superstructures of collagen hydrolysate leads to superior osteoblast proliferation when compared to nano-hydroxyapatite synthesized on collagen fibrils

Contact Info

Product

Resources

About