Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium

Camargo, Samira Esteves Afonso; Xia, Xinyi; Fares, Chaker; Ren, F.; Hsu, Shu‐Min; Budei, Dragoş Vladimir; Aravindraja, Chairmandurai; Kesavalu, Lakshmyya; Esquivel‐Upshaw, Josephine F.

doi:10.3390/ma14164357

Cited by 13 publications

(10 citation statements)

References 37 publications

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“…Electrochemical anodization with the production of nanotubes with different diameters and thicknesses of the wall [84][85][86] and laser texturing with a femtosecond laser to create grooves with hundreds of nanometers resolution [87][88][89] are the most used technics of titanium surface nanostructuring.…”

Section: Titaniummentioning

confidence: 99%

Nanomaterials in Bone Regeneration

et al. 2022

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Nanomaterials are promising in the development of innovative therapeutic options that include tissue and organ replacement, as well as bone repair and regeneration. The expansion of new nanoscaled biomaterials is based on progress in the field of nanotechnologies, material sciences, and biomedicine. In recent decades, nanomaterial systems have bridged the line between the synthetic and natural worlds, leading to the emergence of a new science called nanomaterial design for biological applications. Nanomaterials replicating bone properties and providing unique functions help in bone tissue engineering. This review article is focused on nanomaterials utilized in or being explored for the purpose of bone repair and regeneration. After a brief overview of bone biology, including a description of bone cells, matrix, and development, nanostructured materials and different types of nanoparticles are discussed in detail.

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Section: Titaniummentioning

confidence: 99%

Nanomaterials in Bone Regeneration

et al. 2022

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“…The SARS-CoV-2 pandemic has highlighted the need to focus attention on the effect such surfaces can have on people’s health. Specifically, today, a lot of effort has been oriented to the production of materials and surfaces that can limit the growth and proliferation of micro-organisms . Surfaces, in fact, especially in public environments, represent one of the main means for the transmission of pathogens …”

Section: Introductionmentioning

confidence: 99%

Innovative Codeposition of a Ag–Al₂O₃ Layer: An Attractive Combination of High Durability and Lack of Cytotoxicity for Public Space Applications

et al. 2022

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Today, the use of silver in surfaces for public environments is very frequent, as it ensures high antimicrobial activities, avoiding the continuous disinfection of the surfaces themselves. Similarly, thanks to its interesting combination of technological properties, anodized aluminum is widely employed in the production of components for applications in public spaces. Therefore, this work describes a simple method of the codeposition of silver and anodized aluminum to combine the remarkable properties of Al 2 O 3 layers with the antibacterial performances of silver. The effect of silver in modifying the durability features of the anodized aluminum layer was evaluated by means of various accelerated degradation techniques, such as the exposure in a climatic chamber to UV-B radiation or an aggressive atmosphere simulated by the Kesternich test. These analyses showed the good compatibility between Ag and the alumina matrix, whose durability performances were not particularly influenced by silver. Furthermore, the composite layers did not express relevant cytotoxicity activity, as evidenced by Trypan blue flow cytometry analysis and microscopy observations, ensuring the possible use of this material in applications in close contact with humans. This same conclusion was reached by observing an almost negligible ionic release of Ag by the composite layers, even following severe degradation of the alumina matrix due to exposure to a particular acid solution. In conclusion, this work presents an innovative material that can be used in public spaces, thanks to its interesting combination of high durability and low cytotoxicity.

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“…Nanoscale surface modifications of dental implants have been beneficial in improving the degree of osseointegration by increasing the surface roughness and changing the surface chemistry [ 6 ]. Studies have demonstrated that human cells are suitable for interacting with nanostructured surfaces and coating these surfaces with biocompatible thin layers can improve this interaction [ 7 , 8 ]. In addition to surface topography, different elements have been introduced to titanium surfaces for improving their osteogenic activity [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Silicon Carbide Coating on Osteoblast Mineralization of Anodized Titanium Surfaces

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Rocha

Xia

et al. 2022

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The objective of this study was to evaluate the influence of the titanium nanotube diameter and the effect of silicon carbide (SiC) coating on the proliferation and mineralization of pre-osteoblasts on titanium nanostructured surfaces. Anodized titanium sheets with nanotube diameters of 50 and 100 nm were used. The following four groups were tested in the study: (1) non-coated 50 nm nanotubes; (2) SiC-coated 50 nm titanium nanotubes; (3) non-coated 100 nm nanotubes and (4) SiC-coated 100 nm nanotubes. The biocompatibility and cytotoxicity of pre-osteoblasts were evaluated using a CellTiter-BlueCell Viability assay after 1, 2, and 3 days. After 3 days, cells attached to the surface were observed by SEM. Pre-osteoblast mineralization was determined using Alizarin-Red staining solution after 21 days of cultivation. Data were analyzed by a Kruskal–Wallis test at a p-value of 0.05. The results evidenced biocompatibility and non-cytotoxicity of both 50 and 100 nm diameter coated and non-coated surfaces after 1, 2 and 3 days. The statistical analysis indicates a statistically significant higher cell growth at 3 days (p < 0.05). SEM images after 3 days demonstrated flattened-shaped cells without any noticeable difference in the phenotypes between different diameters or surface treatments. After 21 days of induced osteogenic differentiation, the statistical analysis indicates significantly higher osteoblast calcification on coated groups of both diameters when compared with non-coated groups (p < 0.05). Based on these results, we can conclude that the titanium nanotube diameter did not play any role on cell viability or mineralization of pre-osteoblasts on SiC-coated or non-coated titanium nanotube sheets. The SiC coating demonstrated biocompatibility and non-cytotoxicity and contributed to an increase in osteoblast mineralization on titanium nanostructured surfaces when compared to non-coated groups.

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Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium

Cited by 13 publications

References 37 publications

Nanomaterials in Bone Regeneration

Nanomaterials in Bone Regeneration

Innovative Codeposition of a Ag–Al₂O₃ Layer: An Attractive Combination of High Durability and Lack of Cytotoxicity for Public Space Applications

Effect of Silicon Carbide Coating on Osteoblast Mineralization of Anodized Titanium Surfaces

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Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium

Cited by 13 publications

References 37 publications

Nanomaterials in Bone Regeneration

Nanomaterials in Bone Regeneration

Innovative Codeposition of a Ag–Al2O3 Layer: An Attractive Combination of High Durability and Lack of Cytotoxicity for Public Space Applications

Effect of Silicon Carbide Coating on Osteoblast Mineralization of Anodized Titanium Surfaces

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Innovative Codeposition of a Ag–Al₂O₃ Layer: An Attractive Combination of High Durability and Lack of Cytotoxicity for Public Space Applications